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Radix ranunculi ternati crude polysaccharide and polysaccharide component as well as preparation method of polysaccharide component as well as application of radix ranunculi ternati crude polysaccharide and polysaccharide component in liver protection and immune regulation

A technology of cat's claw and crude polysaccharides, applied in the fields of traditional Chinese medicine, hepatology and immunology, to reduce liver inflammation, inhibit expression, and reduce environmental pollution

Active Publication Date: 2018-09-28
SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, in terms of biological activity, it is limited to the in vitro evaluation of the inhibitory effect of cat's claw crude polysaccharides on tumor cell growth and the stimulating activity of immune cells

Method used

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  • Radix ranunculi ternati crude polysaccharide and polysaccharide component as well as preparation method of polysaccharide component as well as application of radix ranunculi ternati crude polysaccharide and polysaccharide component in liver protection and immune regulation
  • Radix ranunculi ternati crude polysaccharide and polysaccharide component as well as preparation method of polysaccharide component as well as application of radix ranunculi ternati crude polysaccharide and polysaccharide component in liver protection and immune regulation
  • Radix ranunculi ternati crude polysaccharide and polysaccharide component as well as preparation method of polysaccharide component as well as application of radix ranunculi ternati crude polysaccharide and polysaccharide component in liver protection and immune regulation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: the preparation of cat's claw crude polysaccharide (1)

[0057] Step 1: Water Extraction

[0058] The raw medicinal material of cat's claw grass root is crushed and passed through a 20-mesh sieve. Weigh 3 parts of cat's claw root powder, each 1kg. Add 15L of distilled water for extraction at room temperature of 20°C, 60°C and 100°C respectively. Among them, 24 hours of leaching at room temperature, 4 hours of leaching at 60°C, 1.5 hours of decocting at 100°C, and stirring during the leaching. The extract was filtered, and the filter residues were extracted again under the same conditions. The two extracted water extracts were combined and centrifuged (3000r / min×20min), and the supernatants obtained at different temperatures were respectively concentrated to 1.5L under reduced pressure at 50-55°C to obtain a water extract concentrate.

[0059] Step 2: alcohol precipitation

[0060] Add 3 times the volume of ethanol to the cat's claw concentrate extract...

Embodiment 2

[0063] Embodiment 2: the preparation of cat's claw crude polysaccharide (two)

[0064] Step 1: Water Extraction

[0065] The raw medicinal material of cat's claw grass root is crushed and passed through a 20-mesh sieve. Weigh 1kg of cat's claw root powder, add 15L of distilled water, stir and extract at 60°C for 5 hours, then filter. Add 15L of water to the filter residue, and repeat the leaching once under the same conditions. Combine the filtrates, centrifuge (3000r / min×20min), and concentrate the supernatant to 1.5L under reduced pressure at 50-55°C to obtain a 60°C water-extraction concentrate. Add 15L of water to the medicinal material residue, decoct at 100°C for 1.5 hours, filter, centrifuge the filtrate, and concentrate the supernatant to 1.5L under reduced pressure at 50-55°C to obtain a water-extracted concentrate at 100°C.

[0066] Step 2: alcohol precipitation

[0067] Add 3 times the volume of ethanol to the 60°C water-extraction concentrate and the 100°C wate...

Embodiment 3

[0070] Embodiment 3: the preparation of cat's claw crude polysaccharide (three)

[0071] Step 1: Water Extraction

[0072] The original medicinal material of cat's claw root is crushed, passed through a 30-mesh sieve, 1kg of cat's claw root powder is taken, added to 20L of distilled water, stirred and extracted at 10°C for 48 hours, and then filtered. Add 20L of water to the filter residue, and repeat the leaching once under the same conditions. Stirring is performed during the leaching. The water extracts extracted twice were combined, centrifuged (3000r / min×20min), and concentrated under reduced pressure at 55°C to 1.5L to obtain a water extract concentrate.

[0073] Step 2: alcohol precipitation

[0074]Add ethanol to the concentrated solution to make the final concentration of ethanol 80% (the mass concentration of ethanol). After standing for 55 hours, centrifuge (3000r / min×20min) to separate the precipitate. Add the precipitated part to 1.0L water and stir to dissolve...

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Abstract

The invention relates to radix ranunculi ternati crude polysaccharide and polysaccharide component as well as a preparation method of the polysaccharide component and application of the radix ranunculi ternati crude polysaccharide and the polysaccharide component in liver protection and immune regulation. The radix ranunculi ternati crude polysaccharide is extracted from radix ranunculi ternate root by using a water extraction mode. The radix ranunculi ternati crude polysaccharide is further separated to obtain the polysaccharide component. The experiment shows that the radix ranunculi ternaticrude polysaccharide and the polysaccharide component thereof disclosed by the invention have the advantages that the proliferation and cytokine secretion of mouse spleen cells can be promoted; the proliferation of human hepatocytes is promoted and the damage of a toxic agent to the cells is reduced; the expression of a hepatitis b surface antigen and a core antigen is inhibited; the proliferation of hepatoma cells is inhibited; in addition, the damage caused by ConA to liver and kidneys of mice is also inhibited, so that the radix ranunculi ternati crude polysaccharide and the polysaccharidecomponent can be used for preparing drugs or health food for resisting hepatitis B, liver injury and tumor, protecting liver, promoting hepatocyte regeneration or realizing immune regulation.

Description

technical field [0001] The invention belongs to the fields of traditional Chinese medicine, hepatology and immunology, and specifically relates to crude polysaccharides and polysaccharide components of cat's claw, a preparation method thereof, and uses for liver protection and immune regulation. Background technique [0002] Cat's claw is the root tuber of Ranunculus ternatus Thunb., aliased as Sansancao, cat's claw, cat's claw, duck foot and golden flower grass. Cat's claw is warm in nature, sweet and pungent in taste, and has the effects of reducing swelling and dissipating stagnation, clearing away heat and detoxifying. Clinical cat's claw is used to treat lymphatic tuberculosis, tuberculosis, tumors, etc. It can be used alone to treat furunculosis, acute and chronic pharyngitis, postpartum transient blindness, and falciparum malaria [Chen Bingluan, et al. China Wild Plant Resources, 2002, 21(4): 7-9]. Studies have shown that cat's claw contains lactones, polysaccharide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16A61P31/20A61P35/00A61P37/02A23L33/125
CPCA23V2002/00A61K31/715A23L33/125A61P1/16A61P31/20A61P35/00A61P37/02C08B37/0003C08B37/006A23V2200/30A23V2250/51A23V2250/21
Inventor 高月求刘晓燕单俊杰李曼何潇陈彦旭周振华高亚婷麻浩赵修南孙学华张鑫
Owner SHUGUANG HOSPITAL AFFILIATED WITH SHANGHAI UNIV OF T C M
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