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Liquid-nitrogen freezing die and soft biological tissue mechanical test device

A liquid nitrogen freezing and mold technology, which is applied in the direction of measuring devices, test sample preparation, scientific instruments, etc., can solve the problems of clamping local tissue deformation, tissue slippage, and affecting the accuracy of mechanical test results of biological samples, etc. achieve the effect of avoiding damage

Pending Publication Date: 2018-09-28
GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Practice has proved that when the clamping force is too small, the tissue is prone to slipping; when the clamping force is too large, the local tissue is damaged due to stress concentration, resulting in deformation, advanced damage or fracture of the clamped local tissue; when the tensile stress is applied to the tissue, The core part and peripheral part of the tissue in the clamping area form a complex shear force, and it is impossible to accurately measure the change in mechanical properties of the tested sample due to simple experimental processing factors
The above-mentioned clamping and fixing methods have seriously affected the accuracy of the mechanical test results of biological samples. How to effectively fix soft biological tissues to facilitate the accurate measurement of their mechanical properties is urgently needed for a reliable fixed test device.

Method used

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  • Liquid-nitrogen freezing die and soft biological tissue mechanical test device
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  • Liquid-nitrogen freezing die and soft biological tissue mechanical test device

Examples

Experimental program
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Effect test

no. 1 example

[0035] Please refer to figure 1 and figure 2 , the present embodiment provides a liquid nitrogen freezing mold 100 for clamping a biological sample 500 , which includes a first U-shaped block 110 , a second U-shaped block 120 and a fixed sealing assembly 130 .

[0036] The biological sample 500 is a sample that requires a mechanical test, and the biological sample 500 is prefabricated into a rectangular parallelepiped (8×10 -2 ×10 -2 ×10 -2 m), the cuboid biological sample 500 includes two end faces and four peripheral faces. The biological sample 500 in this embodiment includes, for example, muscles, blood vessels, nerves, tendons, etc., and salmon meat is used as the biological sample 500 in this embodiment.

[0037] The first U-shaped block 110 and the second U-shaped block 120 have the same structure, and are used to accommodate the two ends of the biological sample 500 respectively. The two ends are fixed but the middle is not fixed, which is beneficial for subseque...

no. 2 example

[0063] Please refer to Figure 6 , the present embodiment provides a liquid nitrogen freezing mold 200, which is substantially the same as the liquid nitrogen freezing mold 100 of the first embodiment, the difference between the two is that the fixed sealing assembly 230 of the liquid nitrogen freezing mold 200 of the present embodiment only includes a bottom Seal block 231 and top seal block 232 .

[0064] In this embodiment, the bottom sealing block 231 is provided with a U-shaped block mounting platform 2311, and the top sealing block 232 is provided with a U-shaped block receiving groove 2321. The depth of the U-shaped block receiving groove 2321 is greater than that of the first U-shaped block 110 and the second U-shaped block. The height of the block 120, the first U-shaped block 110 and the second U-shaped block 120 are respectively installed on the two ends of the U-shaped block mounting platform 2311, and the top sealing block 132 cooperates with the bottom sealing bl...

no. 3 example

[0067] Please refer to Figure 7 , the present embodiment provides a liquid nitrogen freezing mold 300, which is substantially the same as the liquid nitrogen freezing mold 100 of the first embodiment, the difference between the two is that the fixed sealing assembly 330 of the liquid nitrogen freezing mold 300 of this embodiment only includes a bottom Seal block 331 and top seal block 332 .

[0068] In this embodiment, the bottom sealing block 331 is provided with a U-shaped block installation groove 3311, and the top sealing block 332 is provided with a U-shaped block covering groove 3321. The depth of the U-shaped block installation groove 3311 is smaller than that of the first U-shaped block 110 and the second U-shaped block. The height of the U-shaped block 120, the first U-shaped block 110 and the second U-shaped block 120 are respectively installed at the two ends of the U-shaped block mounting groove 3311, and now the first U-shaped block 110 and the second U-shaped bl...

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Abstract

The invention relates to the field of biological sample mechanical test equipment, and provides a liquid-nitrogen freezing die and a soft biological tissue mechanical test device. The liquid-nitrogenfreezing die comprises a first U-shaped block, a second U-shaped block and a fixed sealing assembly, wherein the first U-shaped block and the second U-shaped block are respectively used for accommodating two ends of a biological sample; the first U-shaped block and the second U-shaped block are arranged in a spacing manner; the fixed sealing assembly comprises a bottom sealing block and a top sealing block; the bottom sealing block and the top sealing block are connected with the first U-shaped block and the second U-shaped block at the same time and surround the four circumferential surfacesof the biological sample. The liquid-nitrogen freezing die can effectively fix the two end parts of the biological sample so as to avoid injury to a biological tissue; meanwhile, the temperature of the middle part of the tissue is equal to that of the peripheral environment, so that the tested sample can be in a natural state to the maximum extent. The soft biological tissue mechanical test devicecomprising the liquid-nitrogen freezing die can effectively clamp and fix the biological tissue and measure the mechanical characteristics of the biological tissue.

Description

technical field [0001] The invention relates to the field of mechanical testing equipment for biological samples, in particular to a liquid nitrogen freezing mold and a soft biological tissue mechanical testing device. Background technique [0002] Mechanical testing studies on biological samples under stress can obtain qualitative information on how different tissues and structures deform or rupture, and these results will help to understand the mechanical properties of complex biological samples. The mechanical performance parameters of soft biological tissues such as muscles, blood vessels, nerves, tendons, and ligaments are often obtained through tensile tests. [0003] In traditional mechanical testing, when fixing soft biological samples, the mechanical clamp method is often used to directly fix biological samples. Practice has proved that when the clamping force is too small, the tissue is prone to slipping; when the clamping force is too large, the local tissue is d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/42G01N3/04
CPCG01N1/42G01N3/04
Inventor 宋越郑晶晶孙静莉张爽金勇
Owner GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
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