Characterization of mir-9 overexpressing tumors and their treatment and specific markers
A marker and characterization technology, applied in the field of pharmacy, can solve problems such as unclear role of exosomes
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0168] The present invention will be further illustrated by the following embodiments in conjunction with the accompanying drawings in order to provide a basis for better understanding of the present invention.
[0169] 1. Method
[0170] The serial numbers before the subheadings are for ease of reading and are for reference only and do not determine the order.
[0171] 1.1 Cell culture
[0172] HUVECs (Allcells, Shanghai, China) were cultured using HUVEC medium (HUVEC-004; Allcells). We use third to sixth generation HUVECs. The human HCC cell lines SMMC-7721 and Huh7 were purchased from the American Type Culture Collection (ATCC, Manassas, Virginia, USA) and cultured in Dulbecco's Modified Eagle's Nest with 10% fetal bovine serum (FBS; Hyclone). Growth was carried out in Hyclone medium high glucose medium (Hyclone; GE Healthcare, Logan, Utah, USA). Cells were maintained at 5% CO 2 , 37°C.
[0173] The MiR-9 mimic (5'-TCTTTGGTTATCTAGCTGTATGA-3') was inserted into the LV3...
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
refractive index | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com