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Microscopic optical imaging detection method and microscopic optical imaging detection apparatus

A technology of optical imaging and detection methods, which can be used in measuring devices, material analysis through optical means, scientific instruments, etc., and can solve problems such as small field of view

Inactive Publication Date: 2018-10-09
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the field of view of an optical microscope with a resolution of about 1 μm is relatively small.

Method used

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  • Microscopic optical imaging detection method and microscopic optical imaging detection apparatus
  • Microscopic optical imaging detection method and microscopic optical imaging detection apparatus
  • Microscopic optical imaging detection method and microscopic optical imaging detection apparatus

Examples

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Embodiment 1

[0067] An example of the micro-optical imaging detection method of the present invention is introduced below. A liquid microbial sample is placed above a submicron image sensor 2, and the biological sample, such as yeast dilution, is directly placed / smeared on a light-transmitting film on the surface of the image sensor 2, which is equal to direct contact with the image sensor 2 .

[0068] The liquid microbial sample can also be placed on a sample carrier 11 when it is not in direct contact with the image sensor. The distance between the sample carrier 11 and the surface of the image sensor 2 is less than 5 mm, so as to meet the near-field conditions required for imaging. It is prevented that the distance between the biological sample and the surface of the image sensor 2 is too far to cause diffraction when the light passes through the biological sample, thus blurring the image.

[0069] The light source is located above the biological sample, and the light source can be a c...

Embodiment 2

[0073] Another way is to place the microbial sample above the image sensor 2, the distance between the biological sample and the surface of the image sensor 2 is less than 5 mm, the light source 6 is located above the sample, and the light source 6 is three coherent light sources, and three incident directions are used for detection. The biological sample is irradiated with coherent light at a certain angle (such as 90°), and projection images of the sample in three directions are obtained on the image sensor 2, and a three-dimensional image of the biological sample can be reconstructed through the projection images in three directions.

[0074] An example of the micro-optical imaging device of the present invention for generating a two-dimensional image includes a light source 5 , a biological sample 3 , and an image sensor 2 from top to bottom. The biological sample can be directly placed on the surface of the image sensor 2, or placed on a transparent sample carrier 11 (such...

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Abstract

The present invention provides a microscopic optical imaging detection method and a microscopic optical imaging detection apparatus, wherein an image sensor comprising an array formed by a plurality of photosensitive detection micro units is used, and each photosensitive detection micro unit forms a pixel unit. The detection method specifically comprises: placing a to-be-detected substance sampleon the front surface, facing illumination light, of an image sensor, such that the to-be-detected substance sample and the image sensor form a near-field optical imaging environment; carrying out optical projecting and / or photographing on the to-be-detected substance sample by using the image sensor; and determining the presence or the number of a to-be-detected organism by using the characteristic difference between the morphology of the to-be-detected organism in the sample and the surrounding organisms. According to the present invention, the traditional detection method using the optical lens is avoided, the projecting imaging of the microorganism sample can be directly achieved by using the small pixel size image sensor, and the method and the apparatus can be used for the optical detection of micro substances, especially liquid-state microorganisms.

Description

technical field [0001] The present invention relates to a microscopic optical imaging method and device, in particular a lensless optical imaging method and device based on a small pixel size image sensor for optical imaging and detection of tiny substances, especially microorganisms. Background technique [0002] The exploration of the microscopic world has laid the foundation for many major scientific discoveries. Most of the scientific breakthroughs in recent years come from the exploration of the microcosm, and the development of life sciences also partly depends on the cognition of the microcosm. Among them, the direct observation of microscopic substances and microorganisms, especially living microorganisms, is an important means of understanding these substances. [0003] In the microscopic research of matter, such as clinical medicine and microbiological research, the intuitive appearance of microorganisms has a great effect on people's understanding of its various ...

Claims

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Application Information

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IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 闫锋杨程马浩文张丽敏卜晓峰毛成孔祥顺黄枝健李煜乾
Owner NANJING UNIV
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