Marine-organism compound antifreeze peptides and preparation method thereof

A technology of antifreeze polypeptides and marine organisms, applied in the field of food processing, can solve the problems of low antifreeze activity of antifreeze polypeptides and achieve strong antifreeze effects

Inactive Publication Date: 2018-10-16
HAIXIN FOODS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above technical problems, the present invention provides a concentrated solution of marine biological compound antifreeze polypeptide to solve the problem of low antifreeze activity of single biological antifreeze polypeptide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] A marine biological compound antifreeze polypeptide concentrate, which is prepared from the following components according to the following weight ratio: 80 parts by weight of laver fish source compound antifreeze powder, 100 parts by weight of deionized water, 0.001% by weight of citric acid, stable Agent 0.001% by weight,

[0044] Its preparation process is as follows:

[0045] Step 1: Add 100 parts by weight of seaweed antifreeze concentrate, 100 parts by weight of fish-derived antifreeze polypeptide concentrate, and 200 parts by weight of deionized water into the mixer, stir for 0.5 h, and centrifugally filter to obtain the supernatant;

[0046] Step 2: Place the supernatant in an environment at a temperature of -6°C, add 200 parts by weight of ice cubes, and let it stand

[0047] After 0.5h, collect ice cubes.

[0048] Step 3: After the ice cubes are melted, freeze-dry them to form laver and fish source compound antifreeze powder.

[0049] Step 4: Add seaweed fi...

Embodiment 2

[0053] A marine biological compound antifreeze polypeptide concentrate, which is prepared from the following components according to the following weight ratio: 120 parts by weight of compound antifreeze powder derived from laver fish, 200 parts by weight of deionized water, 0.003% by weight of citric acid, stable Agent 0.005% by weight.

[0054] Its preparation process is as follows:

[0055] Step 1: adding 200 parts by weight of seaweed antifreeze concentrate, 200 parts by weight of fish-derived antifreeze polypeptide concentrate, and 500 parts by weight of deionized water into the mixer in sequence, stirring for 1 hour, and centrifuging to obtain the supernatant;

[0056] Step 2: Place the supernatant in an environment at a temperature of 0° C., add 500 parts by weight of ice cubes, and

[0057] After 6 hours, collect ice cubes.

[0058] Step 3: After the ice cubes are melted, freeze-dry them to form laver and fish source compound antifreeze powder.

[0059] Step 4: Add ...

Embodiment 3

[0063] A marine biological compound antifreeze polypeptide concentrate, which is prepared from the following components according to the following weight ratio: 100 parts by weight of laver fish source compound antifreeze powder, 150 parts by weight of deionized water, 0.002% by weight of citric acid, stable Agent 0.003% by weight.

[0064] Its preparation process is as follows:

[0065] Step 1: Add 150 parts by weight of seaweed antifreeze concentrate, 150 parts by weight of fish-derived antifreeze polypeptide concentrate, and 80 parts by weight of deionized water into the mixer, stir for 0.7 hours, and centrifugally filter to obtain the supernatant;

[0066] Step 2: place the supernatant in an environment at a temperature of -3°C, add 300 parts by weight of ice cubes, and

[0067]After 3 hours, collect ice cubes.

[0068] Step 3: After the ice cubes are melted, freeze-dry them to form laver and fish source compound antifreeze powder.

[0069] Step 4: Add seaweed fish sour...

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PUM

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Abstract

The invention belongs to the technical field of food processing, and specifically relates to marine-organism compound antifreeze peptides and a preparation method thereof. The marine-organism compoundantifreeze peptides are prepared by blending the following ingredients in parts by weight: 80-120 parts of nori-and-fish-source compound antifreeze powder, 100-200 parts of deionized water, 0.001-0.003 part of citric acid, and 0.001-0.05 part of a stabilizer. The purpose of the marine-organism compound antifreeze peptides is to solve the problem of low anti-freezing activities of single biological antifreeze peptides.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a marine biological compound antifreeze polypeptide concentrate and a preparation method thereof. Background technique [0002] Antifreeze protein, also known as "ice structure protein", is a kind of active protein that attaches to the surface of ice crystals and inhibits the growth and recrystallization of ice crystals. Antifreeze protein can control the growth of ice crystals, reduce cell damage and maintain the original structure, texture and quality of products. [0003] At present, most of the research on antifreeze proteins is limited to deep-sea fish or other rare plants. Due to the high cost of raw materials, they can only stay in the laboratory research stage, and it is difficult to extract them for industrial application. [0004] According to relevant research, some people have transferred the antifreeze gene into bacteria to obtain antifreeze prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23L33/125
CPCA23L33/125A23L33/18
Inventor 蒋荣龙魏倩婷
Owner HAIXIN FOODS
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