A kind of natural sericin microsphere wrapped by metal-organic coordination polymer and its preparation method and application

A technology of coordination polymer and sericin, applied in the field of biomedical composite materials, can solve the problems of difficult to precisely control the particle size of microspheres, difficult to control the release rate of the loaded drug, difficult to control the shape and size, etc. Low, high yield, uniform particle size effect

Active Publication Date: 2021-06-25
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current methods for preparing sericin microspheres mainly include coagulation precipitation, spray drying and spray freeze-drying cross-linking methods, but these methods have the following disadvantages: 1) The preparation process is complicated and difficult to implement. For example, the spray freeze-drying method requires the sericin The mixed solution is sprayed into -80°C space for coagulation and collection, and the sericin microspheres can be obtained after freeze-drying; 2) The particle size of the prepared microspheres is difficult to control precisely, such as the sericin microspheres prepared by coagulation precipitation method , its shape and size are difficult to control; 3) The release rate of the loaded drug is not easy to control, such as the release rate of drug-loaded sericin microspheres prepared by coagulation precipitation or spray freeze-drying method is too fast

Method used

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  • A kind of natural sericin microsphere wrapped by metal-organic coordination polymer and its preparation method and application
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  • A kind of natural sericin microsphere wrapped by metal-organic coordination polymer and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Synthesis of natural sericin microspheres

[0055] (1) Wash and drain 20 g silkworm cocoons, soak them in 400 mL 0.02 mol / L Na 2 CO 3 In the solution, stir and heat reaction at 100°C and 600rpm for 1 hour, after cooling, centrifuge at 3500 rpm for 5 minutes to remove insoluble precipitates, take the upper clear solution and dialyze it in deionized water for 2-3 days with a 3500 Da dialysis bag, and the solution is dialyzed at 8000 rpm Centrifuge for 10 minutes to remove insoluble precipitates, take the upper clear solution, freeze it in liquid nitrogen, and place it in a vacuum freeze dryer to completely freeze-dry to obtain pure sericin solid;

[0056] (2) Prepare 1 mL of 10 wt% sericin solution, that is, dissolve 100 mg of sericin in 1 mL of deionized water, add it dropwise to corn oil containing 2% emulsifier Tween-20, The water-to-oil ratio is 1:10, that is, 10 mL of corn oil (containing emulsifier Tween-20 200 μL) is used, and emulsified at 800 rpm for...

Embodiment 2

[0062] Example 2: Effects of Different Concentrations of Sericin on the Synthesis of Sericin Microspheres

[0063] (1) Wash and drain 20 g silkworm cocoons, soak them in 400 mL 0.02 mol / L Na 2 CO 3 In the solution, stir and heat reaction at 100°C and 600rpm for 1 hour, after cooling, centrifuge at 3500 rpm for 5 minutes to remove insoluble precipitates, take the upper clear solution and dialyze it in deionized water for 2-3 days with a 3500 Da dialysis bag, and the solution is dialyzed at 8000 rpm Centrifuge for 10 minutes to remove insoluble precipitates, take the upper clear solution, freeze it in liquid nitrogen, and place it in a vacuum freeze dryer to completely freeze-dry to obtain pure sericin solid;

[0064] (2) Prepare 100 μL of 15 wt% sericin solution, that is, dissolve 15 mg of sericin in 100 μL of deionized water, add it dropwise to corn oil containing 2% emulsifier Tween-20, The water-to-oil ratio is 1:10, that is, the corn oil used is 1 mL (containing emulsifie...

Embodiment 3

[0070] Example 3: Effects of different dosages of emulsifiers on the synthesis of sericin microspheres

[0071] (1) Wash and drain 20 g silkworm cocoons, soak them in 400 mL 0.02 mol / L Na 2 CO 3 In the solution, stir and heat reaction at 100°C and 600rpm for 1 hour, after cooling, centrifuge at 3500 rpm for 5 minutes to remove insoluble precipitates, take the upper clear solution and dialyze it in deionized water with a 3500 Da dialysis bag for 2-3 days. Centrifuge for 10 minutes to remove insoluble precipitates, take the upper clear solution, freeze it in liquid nitrogen, and place it in a vacuum freeze dryer to completely freeze-dry to obtain pure sericin solid;

[0072] (2) Prepare 100 μL of 10 wt% sericin solution, that is, dissolve 10 mg of sericin in 100 μL of deionized water, add it dropwise to corn oil containing 6% emulsifier Tween-20, The water-to-oil ratio is 1:10, that is, the corn oil used is 1 mL (containing emulsifier Tween-20 60 μL), emulsified at 800 rpm for...

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Abstract

The invention relates to a sericin microsphere and its preparation method and application, comprising: (1) extracting sericin in silkworm cocoons by high-temperature alkali extraction, and configuring it into a pure sericin solution; (2) making the sericin solution Add corn oil, stir to disperse, add dropwise cross-linking agent to obtain sericin microspheres; (3) add acetone to the mixed emulsion to solidify sericin microspheres; (4) wash and dry to obtain sericin microspheres (5) co-incubating sericin microspheres with drugs to obtain drug-loaded sericin microspheres; (6) adding phenolic substances and metal ions under neutral or slightly alkaline conditions to obtain metal-organic complexes Polymer-coated drug-loaded sericin microspheres. The sericin microsphere prepared by the invention has stable structure, uniform particle size, good dispersion in water, and retains the fluorescence characteristic of the sericin itself. The drug-loaded sericin microspheres encapsulated by metal-organic coordination polymers have the characteristics of high drug-loading rate and pH sensitivity.

Description

technical field [0001] The invention relates to the field of biomedical composite materials, in particular to a natural sericin microsphere, a drug-loaded sericin microsphere wrapped by a metal-organic coordination polymer, and a preparation method and application thereof. Background technique [0002] Silk Sericin (Silk Sericin) is a natural polymer viscous protein wrapped on the surface of silk fibroin in silkworm cocoons, accounting for about 20-30% of the entire silkworm cocoon content, mainly composed of polypeptides with a molecular weight of 24-400 kDa, including 18 kinds of amino acids such as serine, aspartic acid and glycine. For a long time, due to the lack of people's understanding of sericin, it has been treated as waste in the silk weaving industry, wasting a lot of precious resources and causing environmental pollution. In recent years, it has been found that sericin has outstanding performance in moisturizing, antibacterial, anti-oxidation, anti-coagulation,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/42A61K9/51A61K9/50A61L27/22A61L27/54A61L27/50A61L27/18B01J13/14
CPCA61K9/5031A61K9/5169A61K47/42A61L27/18A61L27/227A61L27/50A61L27/54A61L2300/406A61L2300/416A61L2300/43A61L2300/602B01J13/14C08L89/00C08L87/00
Inventor 王琳王征刘佳邓炎李琪琳万超付达安袁野
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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