Microorganism passivation method for remedying heavy metal lead polluted soil

A lead-contaminated soil and microorganism technology, applied in the restoration of contaminated soil, etc., can solve the problems of exogenous microorganism colonization, affecting the soil passivation effect, exogenous microorganisms are easily swallowed by other microorganisms, etc., to improve the colonization ability , improve properties, enhance the effect of stress resistance

Inactive Publication Date: 2018-10-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, microorganisms are used for soil passivation. The biggest problem is the colonization of exogenous microorganisms in the soil. The introduced exogenous microorganisms are easily swallowed by other microorganisms, thus affecting the effect of soil passivation.

Method used

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  • Microorganism passivation method for remedying heavy metal lead polluted soil
  • Microorganism passivation method for remedying heavy metal lead polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 takes a certain polluted soil 1 in the northern suburb of Xuzhou City as an example to specifically illustrate the treatment steps and effects of the method of the present invention.

[0021] The specific steps of the method for repairing heavy metal lead-contaminated soil of the present embodiment are as follows:

[0022] (1) adding trehalose to the culture medium to carry out fermentation and cultivation of Bacillus subtilis, adding the bacterial solution obtained from fermentation to vinegar residue to prepare a microorganism / vinegar residue mixture;

[0023] a. Strain activation: Inoculate Bacillus subtilis on solid plate medium and culture at 30-37°C for 12-24 hours; solid plate medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar 15-20g / L; pH natural.

[0024] b. Seed solution: Bacillus subtilis was inoculated from the solid plate culture medium into LB shaker flasks, and cultured at 30-37° C. for 12-24 hours to prepare the seed so...

Embodiment 2

[0034] Embodiment 2 takes wheat field soil 2 near a leather factory in Xuzhou as an example, and specifically illustrates the processing steps and effects of the method of the present invention.

[0035] The specific steps of the method for repairing heavy metal lead-contaminated soil of the present embodiment are as follows:

[0036] (1) adding trehalose to the culture medium to carry out fermentation and cultivation of Bacillus subtilis, adding the bacterial solution obtained from fermentation to vinegar residue to prepare a microorganism / vinegar residue mixture;

[0037] a. Strain activation: Inoculate Bacillus subtilis on solid plate medium and culture at 30-37°C for 12-24 hours; solid plate medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar 15-20g / L; pH natural.

[0038] b. Seed solution: Bacillus subtilis was inoculated from the solid plate culture medium into LB shaker flasks, and cultured at 30-37° C. for 12-24 hours to prepare the seed solution. ...

Embodiment 3

[0047] Embodiment 3 takes the soil 3 near a glass factory in Xuzhou as an example to specifically illustrate the treatment steps and effects of the method of the present invention.

[0048] The specific steps of the method for repairing heavy metal lead-contaminated soil of the present embodiment are as follows:

[0049] (1) adding trehalose to the culture medium to carry out fermentation and cultivation of Bacillus subtilis, adding the bacterial solution obtained from fermentation to vinegar residue to prepare a microorganism / vinegar residue mixture;

[0050] a. Strain activation: Inoculate radiation-tolerant black yeast F134 on YPD plate medium, and culture at 25-30°C for 5-7 days; YPD plate medium: peptone 20g / L; yeast extract 10g / L; glucose 20g / L; agar powder 16g / L; pH natural.

[0051] b. Seed solution: Inoculate the black yeast F134 from the YPD plate medium into the MEA shake flask, and culture it at 25-30° C. for 48-72 hours to prepare the seed solution. MEA medium: ...

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Abstract

The invention discloses a microorganism passivation method for remedying heavy metal lead polluted soil. The microorganism passivation method comprises the steps that trehalose is added into a culturemedium, microorganism bacteria solution fermentation cultivation is conducted to obtain a microorganism bacteria solution, the microorganism bacteria solution is added into vinegar residues, and a microorganism bacteria solution/vinegar residue mixture is prepared; the microorganism bacteria solution/vinegar residue mixture is evenly spread in the soil, ploughing is conducted, and thus the pH ofthe soil is lowered to acidic; and finally phosphorus-containing materials and sodium silicate are evenly mixed, a mixture of the phosphorus-containing materials and the sodium silicate is evenly spread in the soil, and ploughing is conducted again. Through the microorganism passivation method, colonizing capacity of microorganisms can be improved, operation is simple, the microorganism passivation method is economical, efficient and suitable for acid, alkaline and neutral soil, the properties of the polluted soil are improved, and soil fertility is improved.

Description

technical field [0001] The invention relates to a microbial passivation method for treating soil polluted by heavy metal lead, which is used for bioremediation of soil polluted by heavy metal. Background technique [0002] At present, the problem of soil pollution in our country is becoming more and more serious, and the environmental problem of soil heavy metal pollution has become an obstacle in the development process. Soil heavy metal pollution has the characteristics of poor mobility of pollutants in the soil, long residence time, and inability to be degraded, so it is difficult to treat and recover; most of them are produced in the production process of machine manufacturing, smelting industry, chemical industry, electronics and other industries. Wastewater of heavy metal ions can be enriched into human or livestock through the biological chain, causing many diseases and seriously affecting human health, life safety and the development of livestock economy. [0003] H...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10B09C2101/00
Inventor 江凌金梦蒙黄和朱丽英
Owner NANJING UNIV OF TECH
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