Method for detecting 1,2-diaminobenzene drugs in animal tissues and dairy products
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of diaminobenzene and animal tissue, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of easy promotion and use, ensuring food safety, and simplifying the pretreatment process
Active Publication Date: 2018-10-23
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
View PDF3 Cites 1 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
It is also reported that metabolites of some 1,2-diaminobenzene anthelmintics, such as sulfoxide products and hydroxylated products, still have strong anti-insect activity, even higher than the original form of the drug, and these metabolites also have certain embryotoxicity
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0057] Example 1: Establishment method
[0058] 1 Materials and methods
[0059] 1.1 Materials and Reagents
[0060] 1,2-Diaminobenzene drug standard: phenyl sulfoxide imidazole, benthiodazole, febantel, phenyl sulfoxide imidazole sulfone, oxybendazole, thiabendazole, 5-hydroxythiabendazole , Tribendazole, Tribendazolone, Prothiodazole, Prothiodazole Sulfone, Prothiodazole Sulfoxide, Prothiodazole-2-Amino Sulfone, Flubendazole, 2-Amino 19 kinds of 1,2-diaminobenzenes such as flubendazole, mebendazole, amino mebendazole, hydroxy mebendazole, thiabendazole ester; acetonitrile, formic acid (chromatographically pure); N,N-dimethylformamide (DMF), n-hexane, sodium chloride (analytical grade); deionized water was obtained by ultrapure water machine of Millipore Company.
[0061] 0.1% formic acid solution: Pipet 1 mL of formic acid into a 1 L volumetric flask, make up to the mark with water, and shake well for use.
[0062] 1.2 Instruments and Equipment
[0063] UFLC LC-20A Ultr...
Embodiment 2
[0118] Example 2: Determination of actual samples
[0119] According to the method established in Example 1, 18 parts of mutton, 10 parts of chicken liver, 10 parts of beef kidney, 10 parts of fish meat, 15 parts of sterilized goat milk, 15 parts of raw milk and 15 parts of milk powder currently on the market were tested. Determination. Prothiodazole 155 μg / kg, prothiodazole sulfone 20 μg / kg, prothiodazole-2-aminosulfone 198 μg / kg from 1 chicken liver, see Figure 16-Figure 18 . Mebendazole 20μg / kg was detected in 1 fish meat, see Figure 19 . No other samples were detected. To sum up: this patent establishes a method for the determination of 1,2-diaminobenzenes in animal tissues and dairy products by direct dilution high performance liquid chromatography-tandem mass spectrometry. The method is simple to operate, low cost, high sensitivity, strong anti-interference ability, accurate and reliable measurement results, and is suitable for animal muscle, liver, kidney, aquati...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
concentration
aaaaa
aaaaa
Login to view more
Abstract
In view of the fact that 1,2-diaminobenzene anthelmintic drugs may cause teratogenesis and embryotoxicity in animals including humans, the present invention discloses a method for rapid and accurate determination of amounts of residues of 19 kinds of 1,2-diaminobenzene drugs in animal tissues and dairy products. The method of detecting the drug residues is used to ensure food safety, especially health of sensitive groups such as the elderly, pregnant women and infants. The method for determination of the 19 kinds of 1,2-diaminobenzene drugs in the animal tissues and dairy products is established by technologies such as high performance liquid chromatography-tandem quadrupole mass spectrometry combined with electrospray ionization monitoring. The method is simple in operation, high in sensitivity, strong in anti-interference ability, accurate in qualitation and quantitation, can provide a technical support for the monitoring of the 19 kinds of 1,2-diaminobenzene drugs in the animal tissues and dairy products, helps to strengthen detection technology in China, and provides a technical support for protecting consumers' health and ensuring food safety.
Description
technical field [0001] The invention belongs to the technical field of detection of veterinary drug residues in food, and in particular relates to a detection method of 1,2-diaminobenzene drugs in animal tissues and dairy products. Background technique [0002] 1,2-diaminobenzene drugs are a class of highly efficient and low-toxic anthelmintics. They have a common feature in their chemical structures, that is, they have the same central structure: 1,2-diaminobenzene, namely "benzimidazole" "nucleus", this type of drug is widely used in the breeding industry of food animals (pigs, cattle, sheep, horses, etc.). Benmidazole and thiabendazole etc. Most 1,2-diaminobenzene anthelmintics will be rapidly metabolized in animals, but mebendazole has a low metabolic rate and is mainly excreted as the original drug. The structure of 1,2-diaminobenzene is quite stable. During the biotransformation of 1,2-diaminobenzene anthelmintic drugs in animals, their metabolic reactions all occur ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 吴映璇林峰陈思敏邵琳智欧阳少伦蓝草
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Login to view more