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Method for detecting 1,2-diaminobenzene drugs in animal tissues and dairy products

A technology of diaminobenzene and animal tissue, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of easy promotion and use, ensuring food safety, and simplifying the pretreatment process

Active Publication Date: 2018-10-23
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also reported that metabolites of some 1,2-diaminobenzene anthelmintics, such as sulfoxide products and hydroxylated products, still have strong anti-insect activity, even higher than the original form of the drug, and these metabolites also have certain embryotoxicity

Method used

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  • Method for detecting 1,2-diaminobenzene drugs in animal tissues and dairy products
  • Method for detecting 1,2-diaminobenzene drugs in animal tissues and dairy products
  • Method for detecting 1,2-diaminobenzene drugs in animal tissues and dairy products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Establishment method

[0058] 1 Materials and methods

[0059] 1.1 Materials and Reagents

[0060] 1,2-Diaminobenzene drug standard: phenyl sulfoxide imidazole, benthiodazole, febantel, phenyl sulfoxide imidazole sulfone, oxybendazole, thiabendazole, 5-hydroxythiabendazole , Tribendazole, Tribendazolone, Prothiodazole, Prothiodazole Sulfone, Prothiodazole Sulfoxide, Prothiodazole-2-Amino Sulfone, Flubendazole, 2-Amino 19 kinds of 1,2-diaminobenzenes such as flubendazole, mebendazole, amino mebendazole, hydroxy mebendazole, thiabendazole ester; acetonitrile, formic acid (chromatographically pure); N,N-dimethylformamide (DMF), n-hexane, sodium chloride (analytical grade); deionized water was obtained by ultrapure water machine of Millipore Company.

[0061] 0.1% formic acid solution: Pipet 1 mL of formic acid into a 1 L volumetric flask, make up to the mark with water, and shake well for use.

[0062] 1.2 Instruments and Equipment

[0063] UFLC LC-20A Ultr...

Embodiment 2

[0118] Example 2: Determination of actual samples

[0119] According to the method established in Example 1, 18 parts of mutton, 10 parts of chicken liver, 10 parts of beef kidney, 10 parts of fish meat, 15 parts of sterilized goat milk, 15 parts of raw milk and 15 parts of milk powder currently on the market were tested. Determination. Prothiodazole 155 μg / kg, prothiodazole sulfone 20 μg / kg, prothiodazole-2-aminosulfone 198 μg / kg from 1 chicken liver, see Figure 16-Figure 18 . Mebendazole 20μg / kg was detected in 1 fish meat, see Figure 19 . No other samples were detected. To sum up: this patent establishes a method for the determination of 1,2-diaminobenzenes in animal tissues and dairy products by direct dilution high performance liquid chromatography-tandem mass spectrometry. The method is simple to operate, low cost, high sensitivity, strong anti-interference ability, accurate and reliable measurement results, and is suitable for animal muscle, liver, kidney, aquati...

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Abstract

In view of the fact that 1,2-diaminobenzene anthelmintic drugs may cause teratogenesis and embryotoxicity in animals including humans, the present invention discloses a method for rapid and accurate determination of amounts of residues of 19 kinds of 1,2-diaminobenzene drugs in animal tissues and dairy products. The method of detecting the drug residues is used to ensure food safety, especially health of sensitive groups such as the elderly, pregnant women and infants. The method for determination of the 19 kinds of 1,2-diaminobenzene drugs in the animal tissues and dairy products is established by technologies such as high performance liquid chromatography-tandem quadrupole mass spectrometry combined with electrospray ionization monitoring. The method is simple in operation, high in sensitivity, strong in anti-interference ability, accurate in qualitation and quantitation, can provide a technical support for the monitoring of the 19 kinds of 1,2-diaminobenzene drugs in the animal tissues and dairy products, helps to strengthen detection technology in China, and provides a technical support for protecting consumers' health and ensuring food safety.

Description

technical field [0001] The invention belongs to the technical field of detection of veterinary drug residues in food, and in particular relates to a detection method of 1,2-diaminobenzene drugs in animal tissues and dairy products. Background technique [0002] 1,2-diaminobenzene drugs are a class of highly efficient and low-toxic anthelmintics. They have a common feature in their chemical structures, that is, they have the same central structure: 1,2-diaminobenzene, namely "benzimidazole" "nucleus", this type of drug is widely used in the breeding industry of food animals (pigs, cattle, sheep, horses, etc.). Benmidazole and thiabendazole etc. Most 1,2-diaminobenzene anthelmintics will be rapidly metabolized in animals, but mebendazole has a low metabolic rate and is mainly excreted as the original drug. The structure of 1,2-diaminobenzene is quite stable. During the biotransformation of 1,2-diaminobenzene anthelmintic drugs in animals, their metabolic reactions all occur ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 吴映璇林峰陈思敏邵琳智欧阳少伦蓝草
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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