Method for quickly screening antibacterial medicine residue in milk-like liquid sample

Abstract

The invention discloses a method for quickly screening antibacterial drug residues in milk liquid samples, which comprises: preparing a spore liquid for detection, preparing an agar medium detection tube containing working bacteria and an indicator, and preparing a liquid medium for detection; Using the principle that antibacterial drugs can inhibit the growth or reproduction of bacteria sensitive to it, use a quantitative pipette to draw the liquid sample to be tested and drop it into the agar medium detection tube and add the liquid medium for detection. State culture for a certain period of time, according to the nature and degree of the color change of the solid agar medium in the detection tube, it is judged whether the sample contains antibacterial drug residues. Compared with the prior art, the present invention has the characteristics of fast and simple detection method, low interference, wide detection spectrum, high sensitivity, high accuracy, good stability, etc., and is suitable for high-throughput and rapid detection of antimicrobial drug residues in milk liquid sample agricultural products. Screening test.

Description

technical field [0001] The invention relates to the technical field of detection and analysis of antimicrobial drug residues, in particular to a method for rapidly screening antimicrobial drug residues in milk liquid samples by utilizing the inhibition of bacterial growth by the residual antimicrobial drugs in a sample. Background technique [0002] Antimicrobial drug residues have always been a serious problem that plagues food safety. Dairy farms widely use antimicrobial drugs to treat various infectious diseases, so it is easy to cause drug residues in milk. At present, the methods used to detect antibacterial drugs in milk mainly include high performance liquid chromatography, liquid phase-mass spectrometry, gas chromatography-mass spectrometry, thin-layer chromatography and other chromatographic methods. The main disadvantages of these chromatographic methods are: professional equipment is required; sample pretreatment procedures are cumbersome; the operation process is...

AI Technical Summary

Problems solved by technology

The main disadvantages of these chromatographic methods are: professional equipment is required; sample pretreatment procedures are cumbersome; the operation process is complicated; time-consuming; professionally trained technicians are required to operate; there are many interference factors; analytical instruments are expensive; it is difficult to popularize at the grassroots level. Generally only suitable for laboratory testing; testing costs are high

However, in the specific operation process of this method, adding the number of Streptococcus thermophilus in the milk will have a great impact on the detection results, resulting in false negative or false positive results. At the same time, the detection time is also long, which is laborious