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A method for detecting 1,2-diaminobenzenes in animal tissues and dairy products

A technology of diaminobenzene and animal tissue, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2021-04-27
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is also reported that metabolites of some 1,2-diaminobenzene anthelmintics, such as sulfoxide products and hydroxylated products, still have strong anti-insect activity, even higher than the original form of the drug, and these metabolites also have certain embryotoxicity

Method used

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  • A method for detecting 1,2-diaminobenzenes in animal tissues and dairy products
  • A method for detecting 1,2-diaminobenzenes in animal tissues and dairy products
  • A method for detecting 1,2-diaminobenzenes in animal tissues and dairy products

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Establishment method

[0058] 1 Materials and methods

[0059] 1.1 Materials and reagents

[0060] 1,2-Diaminobenzene drug standard products: phenyl sulfoxide imidazole, fensulfidazole, febantyr, phenyl sulfoxide imidazole sulfone, oxybendazole, thiabendazole, 5-hydroxythiabendazole , Triclobendazole, Triclosendazolone, Prothiodazole, Prothiodazole Sulfone, Prothiodazole Sulfoxide, Prothiodazole-2-Aminosulfone, Flubendazole, 2-Amino 19 kinds of 1,2-diaminobenzene drugs such as flubendazole, mebendazole, aminomethbendazole, hydroxymesbendazole, thiabendazole; acetonitrile, formic acid (chromatographically pure); N,N-dimethylformamide (DMF), n-hexane, sodium chloride (analytical pure); deionized water was made by Millipore company ultrapure water machine.

[0061] 0.1% formic acid solution: draw 1mL of formic acid into a 1L volumetric flask, dilute to the mark with water, shake well and set aside.

[0062] 1.2 Instruments and equipment

[0063] UFLC LC-20A...

Embodiment 2

[0119] Embodiment 2: the mensuration of actual sample

[0120] According to the method established in Example 1, 18 parts of mutton, 10 parts of chicken liver, 10 parts of beef kidney, 10 parts of fish meat, 15 parts of sterilized goat's milk, 15 parts of fresh cow's milk and 15 parts of milk powder samples sold in the market were carried out Determination. From one piece of chicken liver, 155 μg / kg of prothiodazole, 20 μg / kg of prothiodazole sulfone, and 198 μg / kg of prothiodazole-2-aminosulfone were detected, see Figure 16-Figure 18 . Mebendazole 20 μg / kg was detected from 1 portion of fish meat, see Figure 19 . No other samples were detected. In summary: this patent establishes a method for the determination of 1,2-diaminobenzenes in animal tissues and dairy products by direct dilution high performance liquid chromatography-tandem mass spectrometry. The method is easy to operate, low cost, high sensitivity, strong anti-interference ability, accurate and reliable meas...

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Abstract

In view of the fact that 1,2-diaminobenzene anthelmintic drugs may cause teratogenicity and embryotoxicity in animals including humans, the invention discloses a method for the rapid and accurate determination of 19 kinds of 1,2-diaminobenzene in animal tissues and dairy products The detection method of drug residues is used to ensure food safety, especially the health of sensitive groups such as the elderly, pregnant women and infants. This patent uses high performance liquid chromatography-tandem quadrupole mass spectrometry combined with electrospray positive ion monitoring and other technologies to establish a detection method for 19 kinds of 1,2-diaminobenzene drugs in animal tissues and dairy products. The method is easy to operate, has high sensitivity, strong anti-interference ability, and accurate qualitative and quantitative analysis. It can provide technical support for the monitoring of 19 kinds of 1,2-diaminobenzene drugs in animal tissues and dairy products, and will help to strengthen my country's Testing technology provides technical support for protecting the health of consumers and ensuring food safety.

Description

technical field [0001] The invention belongs to the technical field of detection of veterinary drug residues in food, and in particular relates to a detection method of 1,2-diaminobenzene drugs in animal tissues and dairy products. Background technique [0002] 1,2-diaminobenzene drugs are a class of highly efficient and low-toxic anthelmintics. They have a common feature in their chemical structures, that is, they have the same central structure: 1,2-diaminobenzene, namely "benzimidazole" "nucleus", this type of drug is widely used in the breeding industry of food animals (pigs, cattle, sheep, horses, etc.). Benmidazole and thiabendazole etc. Most 1,2-diaminobenzene anthelmintics will be rapidly metabolized in animals, but mebendazole has a low metabolic rate and is mainly excreted as the original drug. The structure of 1,2-diaminobenzene is quite stable. During the biotransformation of 1,2-diaminobenzene anthelmintic drugs in animals, their metabolic reactions all occur ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 吴映璇林峰陈思敏邵琳智欧阳少伦蓝草
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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