Counter-based high-precision bacteria solution preparation method

Abstract

The invention discloses a counter-based high-precision bacteria solution preparation method, and belongs to the technical field of medication experiments. The method is characterized by including thesteps: a, preparing standard micro-sphere turbid liquid; b, preparing bacteria solution; c, acquiring mixed solution of the standard micro-sphere turbid liquid and the bacteria solution; d, acquiringthe number of standard micro-spheres and bacteria in the mixed solution; e, acquiring the value of the bacterial concentration of the bacteria solution in the step b; f, preparing the bacteria solution with preset bacterial concentration according to the bacterial concentration of the bacteria solution in the step b. According to the counter-based high-precision bacteria solution preparation method, the bacterial concentration of the bacteria solution is accurately calculated by setting the standard micro-sphere turbid liquid, mixing the standard micro-sphere turbid liquid and the bacteria solution and then comparing the number of the standard micro-spheres and the bacteria, drug efficacy can be determined according to known accurate bacterial concentration, and precise medication can be realized.

Description

technical field [0001] The invention relates to a method for preparing high-precision bacterial liquid based on counting, which belongs to the technical field of medication experiments. Background technique [0002] At present, in hospital laboratories, in order to determine the sterilization effect of a certain drug, the conventional method is to prepare a certain concentration of bacterial liquid, and then combine a certain concentration of the drug with the bacterial liquid for testing. Since the drug is generally prepared in strict accordance with the relevant requirements when it leaves the factory, the concentration unit of the drug is very accurate when it leaves the factory, so the concentration of the bacterial solution that is mixed with it is one of the decisive factors that affect the experimental results. [0003] In the prior art, there are two recognized methods for determining the concentration of bacterial liquid: turbidimetric method and absorbance method, ...

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Problems solved by technology

[0004] However, in the actual test, due to the large difference in the shape and volume of various bacteria (such as spherical bacteria, rod-shaped bacteria, and spiral bacteria), in the same volume of bacterial liquid, the turbidimetric method or absorbance method is used to measure The actual concentration (the number of bacteria in the bacterial liquid per unit volume) will be quite different due to the different bacterial forms in the same concentration of bacterial liquid. Therefore, the bacteria in the bacterial liquid measured by the method of the prior art Concentration data will have a huge error

[0005] As can be seen from the above, since the data of the bacterial concentration in the bacterial liquid cannot be accurately measured, it will greatly affect the determination of the efficacy of the drug, and it is impossible to obtain sufficient understanding of the efficacy of the drug, thus further affecting the clinical dosage and medication. The selection of types cannot achieve precise drug use

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