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Method for preventing and treating viral diseases of petunia

A technology for petunia and virus diseases, applied in the field of plant virus prevention and control, can solve the problems of cumbersome use method, low efficiency, unfavorable industrialization and the like

Inactive Publication Date: 2018-11-13
常鑫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the methods of inoculation mostly adopt root soaking, spray gun and friction inoculation. Before inoculation of weak virus, seed disinfection and seedbed control of aphids and whitefly must be carried out to prevent seedlings from being infected by strong virus before inoculation of weak virus, which is time-consuming and expensive. The method is cumbersome, low efficiency and other shortcomings, so it is not conducive to industrialization and cannot meet the needs of modern agriculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The method for the prevention and treatment of petunia virus disease by this weakened virus is carried out as follows:

[0035] 1), the preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter are:

[0036] (1) Basic medium: the basic medium adopts MS medium;

[0037] (2) induction medium;

[0038] (3) proliferation medium;

[0039] (4) strong seedling culture medium;

[0040] (5) Rooting medium.

[0041] 2), virus-free cultivation of petunia shoot tip:

[0042] (1) Selection and sterilization of explants: take the seeds of petunia, inoculate them on the sterile sowing medium after sterilizing, cultivate them for 30 to 45 days under the culture conditions, and cultivate them from the seeds to about 5 ~7cm seedlings can be directly used as explants for shoot tip virus-free culture without sterilization;

[0043] (2) Virus-free culture of stem tips: Under a...

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PUM

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Abstract

The invention relates to a method for preventing and treating viral diseases of petunia. The method comprises the following steps: 1) preparing a culture medium; 2) carrying out virus-free culture onpetunia stem tips; 3) carrying out virus ELISA detection; 4) carrying out weak virus inoculation; and 5) tissue culture propagation of weak virus plants. The method has the beneficial effects that tissue culture propagation of the plants inoculated with weak viruses is carried out by a plant tissue culture technology, the bred tissue culture seedlings completely carry weak viruses, and can be continuously bred as production seeds, the step of carrying out immunization before production in the past years is omitted, and the working efficiency is improved exponentially. The incidence rate of other leaf viruses and similar diseases of the petunia protected by the weak viruses is 3% or below, plants are robust, flowers are large and colorful, and the quality is improved obviously.

Description

technical field [0001] The invention relates to the technical field of plant virus prevention and control, in particular to a method for attenuated virus prevention and treatment of petunia virus disease. Background technique [0002] Petunias are native to South America and belong to the Solanaceae Pittona genus. They are herbaceous plants widely used in potted plants and flower beds. Because of its long flowering period and many varieties of flowers and colors, it is deeply loved by people, and it has high ornamental value in beautifying the environment. However, due to the serious harm of the virus, the affected plants have mottled leaves, forming mosaic leaves, with dark and light colors, often accompanied by curled leaves, weakened growth potential, deformed flowers, and smaller flowers, which greatly reduce their ornamental value. According to investigations, the incidence rate of seed propagation is 20-30%, and the incidence rate of asexual reproduction can be as hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 常鑫
Owner 常鑫