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Treatment method for improving anti-calcification performance of biological material, and biological material

A technology of biomaterials and processing methods, applied in tissue regeneration, medical science, prosthesis, etc., to achieve the effects of improving mechanical strength, improving anti-calcification performance, and improving stability

Inactive Publication Date: 2018-11-13
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, glutaraldehyde-crosslinked biological heart valves are prone to degradation and calcification, resulting in only about 10 years of effective use of biological heart valves

Method used

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  • Treatment method for improving anti-calcification performance of biological material, and biological material
  • Treatment method for improving anti-calcification performance of biological material, and biological material
  • Treatment method for improving anti-calcification performance of biological material, and biological material

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Embodiment 1

[0039] A kind of processing method that present embodiment provides to improve anti-calcification performance of biological material, in all embodiments, fresh pig pericardium comes from local slaughterhouse. Arginine, glutaraldehyde, carbodiimide, elastase, N-hydroxysuccinimide, elastase, calcium chloride, and sodium dihydrogen phosphate were from Chengdu Best Reagent Company. Collagenase was from Sigma-Aldrich.

[0040] Such as figure 1 As shown, the processing method provided in this embodiment includes the following steps:

[0041] S1. Obtain biological materials;

[0042] S2, soak and clean with distilled water;

[0043] S3, then immerse the biological material cleaned in step S2 in an aqueous solution containing multiple amino group small molecular substances and a mass solubility of 0.1-10%;

[0044] S4, then the biological material treated in step S3 is catalyzed by the carboxyl group and multiple amino groups on the pericardium under the catalysis of the mixed sol...

Embodiment 2

[0076] A kind of processing method that present embodiment provides to improve anti-calcification performance of biological material, in all embodiments, fresh pig pericardium comes from local slaughterhouse. Arginine, glutaraldehyde, carbodiimide, elastase, N-hydroxysuccinimide, elastase, calcium chloride, and sodium dihydrogen phosphate were from Chengdu Best Reagent Company. Collagenase was from Sigma-Aldrich.

[0077] The processing method provided by the present embodiment includes the following steps:

[0078] S1. Obtain biological materials;

[0079] S2, soak and clean with distilled water;

[0080] S3, then immerse the biological material cleaned in step S2 in an aqueous solution containing multiple amino group small molecule substances and a mass solubility of 3%;

[0081] S4, then the biological material treated in step S3 is catalyzed by the carboxyl group and multiple amino groups on the pericardium under the catalysis of the mixed solution of carbodiimide / N-hyd...

Embodiment 3

[0086] A kind of processing method that present embodiment provides to improve anti-calcification performance of biological material, in all embodiments, fresh pig pericardium comes from local slaughterhouse. Arginine, glutaraldehyde, carbodiimide, elastase, N-hydroxysuccinimide, elastase, calcium chloride, and sodium dihydrogen phosphate were from Chengdu Best Reagent Company. Collagenase was from Sigma-Aldrich.

[0087] The processing method provided by the present embodiment includes the following steps:

[0088] S1. Obtain biological materials;

[0089] S2, soak and clean with distilled water;

[0090] S3, then immerse the biological material cleaned in step S2 in an aqueous solution containing multiple amino group small molecule substances and a mass solubility of 8%;

[0091] S4, then the biological material treated in step S3 is catalyzed by the mixed solution of carbodiimide / N-hydroxysuccinimide with a molar solubility of 1M through the carboxyl group and multiple a...

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Abstract

The invention discloses a treatment method for improving the anti-calcification performance of biological materials, and the biological materials. The method comprises the steps of soaking the biological materials in an aqueous solution containing a plurality of amino group small molecule substances, wherein small amino molecules permeate into a pericardium material; then, enabling the biologicalmaterials and the plurality of amino group small molecule substances to be subjected to a chemical crosslinking reaction by means of carboxyl on pericardium under the catalytic action of a mixed solution of carbodiimide (EDC) / N-hydroxysuccinimide (NHS). The plurality of amino group small molecule substances are used as an exogenous amino donor, so that more cross-linking points are provided for the biological materials, and the structural stability of the biological materials is further improved. The method provided by the invention can improve the structural stability, mechanical strength andanti-calcification performance of the biological materials, so that the service lives of the biological materials are potentially prolonged.

Description

technical field [0001] The invention relates to a treatment method for improving the anti-calcification performance of biological materials and biological materials thereof, belonging to the technical field of biomedical materials and medical devices. Background technique [0002] Heart valve disease is a common disease of valve degeneration. Anatomically, it manifests as narrowing of blood pathways or valvular insufficiency. [0003] Treatment of heart valve disease includes open heart valve replacement surgery and percutaneous heart valve replacement surgery. Thoracotomy is traumatic, high-risk, slow recovery, and requires extracorporeal circulation support, which is unacceptable to many patients. Percutaneous heart valve replacement surgery has become the main trend of valve surgery in the future because of its small trauma and low risk to patients. [0004] Biological heart valves refer to a class of biomedical materials used to replace human diseased heart valves. B...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/24A61L27/22A61L27/20A61L27/60A61L27/50
CPCA61L27/3604A61L27/20A61L27/222A61L27/227A61L27/24A61L27/362A61L27/3625A61L27/3629A61L27/3641A61L27/3687A61L27/3691A61L27/507A61L27/60A61L2430/20A61L2430/40C08L89/00C08L5/08
Inventor 王云兵雷洋
Owner SICHUAN UNIV
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