Application of PGLYRP1 protein as marker in diagnosis of active tuberculosis

Abstract

The invention discloses application of PGLYRP1 protein as a marker in the diagnosis of active tuberculosis. Compared with healthy people and latent tuberculosis infected people, PGLYRP1 gene expression in PBMCs from active tuberculosis patients is significantly increased. The expression level of PGLYRP1 gene can be used to distinguish between active tuberculosis patients and tuberculosis latent infected people. The expression level of PGLYRP1 gene can be used to distinguish between active tuberculosis patients and healthy people. Therefore, the PGLYRP1 protein and/or PGLYRP1 gene can be used as markers to diagnose active tuberculosis. The invention has great application value.

Description

technical field [0001] The invention belongs to the technical field of medical immunology diagnosis, and in particular relates to the application of PGLYRP1 protein as a marker in the diagnosis of active tuberculosis. Background technique [0002] Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis (MTB), which is mainly transmitted through the respiratory tract. After MTB infects the human body, three different results can occur. One is that the body’s immunity is better, and MTB is directly cleared; the other is that MTB is suppressed by the body’s immune system, but cannot be completely cleared, and develops into latent tuberculosis infection (LTBI). ; The third is that MTB proliferates rapidly in the body and develops into active tuberculosis. Tuberculosis is a major infectious disease that my country needs to focus on prevention and control. [0003] At present, the diagnosis of tuberculosis mainly includes imaging diagnosis, tuberculosis diagno...

AI Technical Summary

Problems solved by technology

However, PPD is an antigen mixture roughly extracted from Mycobacterium tuberculosis, which contains more than 200 proteins, many of which are common antigenic components of non-tuberculous mycobacteria and BCG. Therefore, the specificity of TST detection is poor. False-positive results are prone to occur in vaccinated populations and non-tuberculous mycobacterial-infected populations

TST can only be used as an auxiliary diagnosis according to the strength of the skin reaction, and its sensitivity is only 70-80%

In addition, TST has the disadvantages of time-consuming detection, the need for subject return visit (72h), and the subjective dependence of skin test operation and result interpretation.

IGRA is to quantitatively detect Mycobacterium tuberculosis specific antigens (ESAT6, CFP10 and TB7. 7) The release reaction of IFN-γ detection is used for the diagnosis of tuberculosis infection, but it is difficult for IGRA to distinguish active tuberculosis and latent tuberculosis infection

Failure to diagnose active tuberculosis early will lead to delayed treatment, increased treatment costs and mortality on the one hand; on the other hand, the source of infection cannot be effectively controlled, resulting in the spread of tuberculosis

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