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A time-resolved fluorescent immunochromatographic test strip for detecting safrole and its preparation method and application

An immunochromatographic test strip and time-resolved fluorescence technology, which is applied in analytical materials, fluorescence/phosphorescence, material excitation analysis, etc.

Active Publication Date: 2020-10-30
ZHENGZHOU TOBACCO RES INST OF CNTC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the above methods require advanced detection instruments, the detection costs are expensive, the steps are cumbersome, time-consuming, and require high professionalism of operators, they are not suitable for high-throughput rapid screening detection in grassroots enterprises and institutions.

Method used

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  • A time-resolved fluorescent immunochromatographic test strip for detecting safrole and its preparation method and application
  • A time-resolved fluorescent immunochromatographic test strip for detecting safrole and its preparation method and application
  • A time-resolved fluorescent immunochromatographic test strip for detecting safrole and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 The composition of the time-resolved fluorescent immunochromatographic test strip for detecting safrole

[0039] 1. Test strips

[0040] The test strip is composed of a bottom plate, a sample absorbent pad, a conjugate release pad, a nitrocellulose membrane and an absorbent pad;

[0041] The sample absorbent pad 1, the conjugate release pad 2, the nitrocellulose membrane 3 and the water absorbent pad 4 are overlapped and pasted on the bottom plate 7 in sequence, and 1 / 3 of the conjugate release pad is covered by the sample absorbent pad from the starting end , the end of the conjugate release pad is connected to the beginning of the nitrocellulose membrane, the end of the nitrocellulose membrane is connected to the beginning of the absorbent pad, the beginning of the sample absorbent pad is aligned with the beginning of the PVC bottom plate, and the end of the absorbent pad is connected to the end of the PVC bottom plate alignment;

[0042] The nitrocellulose ...

Embodiment 2

[0044] Example 2 Preparation of time-resolved fluorescent immunochromatographic test strips for detecting safrole

[0045] The preparation method of the time-resolved fluorescent immunochromatography test strip for detecting safrole mainly comprises the following steps:

[0046] 1) Preparation of the conjugate release pad: use commercially available fluorescent microspheres to label safrole monoclonal antibody, dilute it with a specific buffer system, soak the conjugate release pad in the dilution buffer, and prepare it after vacuum freeze-drying ;

[0047] 2) Preparation of nitrocellulose membrane: Spray the safrole hapten-carrier protein conjugate on the detection zone range on the nitrocellulose membrane to make the detection zone; spray goat anti-mouse anti-antibody onto the nitrocellulose membrane The scope of the quality control area is made into the quality control area;

[0048] 3) Assembly and cutting: Paste the sample absorption pad, the conjugate release pad embed...

Embodiment 3

[0100] Example 3 Application of Time-Resolved Fluorescence Immunochromatography Test Strips for Detecting Safrole

[0101] 1. Tobacco sample pretreatment

[0102] Weigh 1.0±0.05 g of crushed tobacco sample into a polystyrene centrifuge tube; add 10 mL of 50% methanol aqueous solution, and use a homogenizer to fully break it up to obtain a sample liquid; pipette 75 μL of sample liquid and 425 μL of The ionized water was mixed and waited for inspection.

[0103] 2. Test with test strips

[0104] Draw 100 μL of the sample solution to be tested and drop it vertically into the sample hole of the test paper card. When the liquid flows, start timing and react for 10 minutes; insert the test paper card into the carrier of the KFT-100A fluorescence detector, and select the sample to be tested through the touch screen. item, press the "Start Test" button, the fluorescence detector will automatically scan the test paper card, and read or print the test results on the display screen of ...

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Abstract

The invention discloses a time resolution fluorescent immunochromatography test strip for detecting safrole and a preparation method and the application of the test strip. The test strip comprises a substrate, a sample absorption pad, a conjugate release pad, a nitrocellulose membrane and a water absorption pad, wherein the sample absorption pad, the conjugate release pad, the nitrocellulose membrane and the water absorption pad are arranged on the substrate in sequence in a lap joint adhesion manner; the conjugate release pad is coated with a safrole monoclonal antibody marked by fluorescentmicrospheres; a detection area and a quality control area are fixed on the nitrocellulose membrane; the detection area is sprayed with safrole hapten-carrier protein conjugate; the quality control area is sprayed with a sheep-anti-mouse anti-antibody; the safrole hapten is prepared from 5-(2-bromine-2-propylene-1-yl)-1,3-benzo dioxolame and aminopropionic acid through reactions. The invention further provides a preparation method of the test strip and a method for detecting safrole in a sample by using the test strip. The test strip and the detection method disclosed by the invention have theadvantages of being simple in operate, high in sensitivity, rapid in detection speed and low in cost, and rapid detection and on-site monitoring on safrole in large-scale samples can be achieved.

Description

technical field [0001] The invention belongs to the field of detection of illegal additives in food safety, and in particular relates to a time-resolved fluorescent immunochromatographic test strip for detecting safrole in tobacco flavors and fragrances, tobacco and tobacco products, and a preparation method and application thereof. Background technique [0002] Safrole, also known as safrole, safrole, is a colorless or slightly yellow liquid, insoluble in water, easily soluble in chloroform, ether and other non-polar organic solvents, with camphor wood smell, naturally occurring in cinnamon, nutmeg, black Plants such as pepper and perilla are the main constituents of many edible natural essential oils such as sassafras, star anise and camphor. Because of its pleasant smell, sassafras oil was once used as a flavoring and fragrance in food, detergents, and cosmetics. Safrole compounds mainly include safrole, isosafrole, dihydrosafrole and so on. Safrole is also an important...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6408G01N2021/641
Inventor 陈黎刘惠民崔娜曹东山樊美娟崔华鹏赵乐贾云祯李行尚平平
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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