Unlock instant, AI-driven research and patent intelligence for your innovation.

Dendrobium candidum polysaccharide extraction process

A Dendrobium officinale polysaccharide and extraction process technology, applied in the field of plant active ingredient extraction, can solve problems such as damage to polysaccharide components, influence on biological activity, lower extraction rate, etc., so as to improve extraction rate, improve extraction rate and purity, and enhance biological active effect

Inactive Publication Date: 2018-11-23
广西驰胜农业科技有限公司
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the polysaccharide extraction of Dendrobium candidum contains many impurities such as protein and pigment, and the purity is low. Too many impurities will affect its biological activity and reduce its quality.
And at this stage, activated c

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The extraction process of Dendrobium officinale polysaccharide in this embodiment includes the following steps:

[0019] (1) Wash fresh Dendrobium officinale, freeze-dried at -30°C, pulverize, pass through a 40-mesh sieve, add 3 times by weight petroleum ether, extract at 60°C until colorless, filter, evaporate the solvent to obtain the medicine Slag, spare.

[0020] (2) Add 3 times the weight of ethanol with a volume concentration of 60% to the above medicine residue, leave it to stand for 5 hours, filter, and evaporate the solvent on the filter residue, add distilled water to reflux for extraction twice, filter, and concentrate the filtrate under reduced pressure To 1 / 3 of the original volume, polysaccharide stock solution, set aside.

[0021] (3) Add 0.05% papain to the above-mentioned polysaccharide stock solution, hydrolyze it in a water bath at 35°C for 1 hour to obtain an enzymatic hydrolysate. Mix the enzymatic hydrolysate, chloroform and n-butanol at 25:4:1 and shake...

Embodiment 2

[0023] The extraction process of Dendrobium officinale polysaccharide in this embodiment includes the following steps:

[0024] (1) Wash fresh Dendrobium officinale, freeze-dried at -20°C, pulverize, pass through a 60-mesh sieve, add 6 weight times petroleum ether, extract at 90°C until colorless, filter, evaporate the solvent to obtain the medicine Slag, spare.

[0025] (2) Add 5 weight times of ethanol with a volume concentration of 70% to the above-mentioned medicine residue, leave it to stand for 7 hours, filter, evaporate the solvent on the residue, add distilled water and reflux for extraction 3 times, filter, and concentrate the filtrate under reduced pressure To 1 / 3 of the original volume, polysaccharide stock solution, set aside.

[0026] (3) Add 0.1% pronase to the above-mentioned polysaccharide stock solution, and hydrolyze it in a water bath at 40°C for 2 hours to obtain an enzymatic hydrolysate. Mix the enzymatic hydrolysate, chloroform and n-butanol at 25:4:1, and shak...

Embodiment 3

[0028] The extraction process of Dendrobium officinale polysaccharide in this embodiment includes the following steps:

[0029] (1) Wash fresh Dendrobium officinale, freeze-dried at -25°C, pulverize, pass through a 50-mesh sieve, add 5 weight times petroleum ether, extract at 70°C until colorless, filter, evaporate the solvent to obtain the medicine Slag, spare.

[0030] (2) Add 4 weight times of ethanol with a volume concentration of 65% to the above-mentioned medicine residue, leave it to stand for 6 hours, filter, evaporate the solvent on the residue, add distilled water and reflux for extraction 3 times, filter, and concentrate the filtrate under reduced pressure To 1 / 3 of the original volume, polysaccharide stock solution, set aside.

[0031] (3) Add 0.06% papain to the above-mentioned polysaccharide stock solution, and hydrolyze it in a water bath at 38°C for 1.5 hours to obtain an enzymatic hydrolysate. Mix the enzymatic hydrolysate, chloroform and n-butanol at 25:4:1, and sh...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a dendrobium candidum polysaccharide extraction process, and relates to the technical field of plant active ingredient extraction. The extraction process comprises the following steps: washing fresh Dendrobium candidum, freeze-drying, pulverizing, adding petroleum ether for extracting till colorless, filtering, evaporating the solvent to obtain dregs, adding ethanol to thedregs, standing for extraction, filtering, evaporating the solvent on the filtered dregs, adding distilled water for reflux extraction for 2 to 3 times, filtering, concentrating the filtrate under reduced pressure to 1/3 of the original volume to obtain a polysaccharide stock solution, adding 0.05% to 0.1% of proteolytic enzyme to the polysaccharide stock solution, hydrolyzing by water bath for 1to 2 hours to obtain enzymatic hydrolysate, uniformly mixing the enzymatic hydrolysate, chloroform and n-butanol at a ratio of 25:4:1, shaking, centrifuging, removing jelly, adding gelatin, filtering,and concentrating and drying the filtrate to obtain dendrobium candidum polysaccharide. The extraction process effectively improves the extraction rate and purity of dendrobium candidum polysaccharide and enhances the biological activity by optimizing the extraction method.

Description

【Technical Field】 [0001] The invention relates to the technical field of extraction of plant active ingredients, in particular to an extraction process of Dendrobium officinale polysaccharides. 【Background technique】 [0002] Dendrobium officinale is a traditional and precious Chinese herbal medicine in my country. It has a medicinal history of more than 2,000 years. Its main chemical components include alkaloids, polysaccharides, polyphenols, etc. Among them, polysaccharides are one of the most effective ingredients in Dendrobium officinale. Pharmacological research On the surface, Dendrobium officinale polysaccharide has strong pharmacological activities such as anti-aging, anti-oxidation, anti-tumor and enhancing body immunity. With the enhancement of people's awareness of health care in recent years, the demand for Dendrobium officinale polysaccharides is constantly increasing, and the market demand is strong, and the supply exceeds demand. It is necessary to further improve t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 谢宗宜
Owner 广西驰胜农业科技有限公司