The invention discloses a preparation method of an anti-oxidation and anti-pollution multi-nutrient block for dairy cows, belonging to the technical field of a block for the dairy cows. The method takes molasses, urea, vitamins and mineral elements as main raw materials; the anti-oxidation and anti-pollution multi-nutrient block is prepared by scientifically compounding natural antioxidant substances, Chinese herb extracts and seasoning extracts; the prepared multi-nutrient block for the dairy cows is moderate in hardness, dampproof, breakage-proof and high in weather fastness; the lapping surface of the block is low in bacterial content which is 1.5 per ten thousand of a comparison group; after the multi-nutrient block is used, the milk yield of the lactating dairy cows can be remarkably increased by 19.02%, the first estrus time is shortened by 13 days after parturition of the dairy cows, the conception rate of the dairy cows at the first estrus period is increased by 16.5% after parturition of the dairy cows, the oestrus proportion of the postpartum dairy cow group is increased by 18.4%, the feed intake and the immunity of the organism of the dairy cows can be effectively improved, the occurrence rates of nutritional diseases such as abnormal addiction disease, foot rot, white muscle disease, postpartum paralysis, rickets, mastitis, coarse fur and the like of the dairy cows can be remarkably reduced, and the occurrence rates are averagely reduced by 3.3-16.7%.