Stauntonia obovatifoliola propagation method

A technology of vine and vine seedlings, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of wild vine resources being on the verge of depletion, and achieve accelerated photosynthetic rhythm, increased pigment content, and survival high rate effect

Active Publication Date: 2015-09-16
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the continuous increase of market demand, the collection of wild resources has gradually increased, and the resources of wild vine resources are on the verge of exhaustion. Therefore, introduction, cultivation and artificial propagation will become new ways to effectively utilize resources of vine resources. See research reports on the cultivation and reproduction of Wuzhina

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A kind of breeding method of vine, comprising the following steps:

[0029] S1: Making sterile explants of Teng chinensis;

[0030] S2: Cultivate the sterile explants using an induction medium, which contains MS, 1mg / L of 6-benzylaminoadenine and 0.5mg / L of indolebutyric acid, to obtain axillary buds;

[0031] S3: Cultivate axillary buds using a proliferation medium containing MS, 0.3mg / L of 1-naphthylacetic acid, 1.5mg / L of TDZ plant growth regulator and 50mg / L of cysteine ​​to obtain clustered buds acid;

[0032] S4: Utilize the strong seedling medium to cultivate the clustered buds to obtain the five-dingled vine seedlings. The strong seedling medium contains MS, 0.1mg / L of 1-naphthylacetic acid, 0.1mg / L of indolebutyric acid and 0.1g / L L of activated carbon;

[0033] S5: Utilize the rooting medium to cultivate the vine seedlings to obtain rooted vine seedlings, wherein the rooting medium contains MS, 0.3 mg / L indole butyric acid and 0.1 g / L of activated carbon; ...

Embodiment 2

[0044] A kind of breeding method of vine, comprising the following steps:

[0045] S1: Making sterile explants of Teng chinensis;

[0046] S2: Cultivate the sterile explants using an induction medium, which contains MS, 2 mg / L of 6-benzylaminoadenine and 1.5 mg / L of indole butyric acid, to obtain axillary buds;

[0047] S3: Cultivate axillary buds using a proliferation medium containing MS, 0.6mg / L of 1-naphthylacetic acid, 2.5mg / L of TDZ plant growth regulator and 100mg / L of cysteine ​​to obtain clustered buds acid;

[0048] S4: Utilize the strong seedling medium to cultivate clustered buds to obtain Wuzhina vine seedlings. The strong seedling medium contains MS, 0.5mg / L of 1-naphthaleneacetic acid, 0.5mg / L of indolebutyric acid and 0.5g / L L of activated carbon;

[0049] S5: Utilize the rooting medium to cultivate the vine seedlings to obtain rooted vine seedlings, wherein the rooting medium contains MS, 1.3mg / L indolebutyric acid and 0.5g / L of activated carbon; The growi...

Embodiment 3

[0060] A kind of breeding method of vine, comprising the following steps:

[0061] S1: Making sterile explants of Teng chinensis;

[0062] S2: Axillary buds were obtained by culturing sterile explants with an induction medium containing MS, 1.5 mg / L of 6-benzylaminoadenine and 1 mg / L of indolebutyric acid.

[0063] S3: Cultivate axillary buds using a proliferation medium containing MS, 0.4mg / L of 1-naphthylacetic acid, 2mg / L of TDZ plant growth regulator and 70mg / L of cysteine ​​to obtain clustered buds .

[0064] S4: Utilize the strong seedling medium to cultivate clustered buds to obtain the vine seedlings. The strong seedling medium contains MS, 0.3mg / L of 1-naphthaleneacetic acid, 0.3mg / L of indolebutyric acid and 0.3g / L L activated carbon.

[0065] S5: Cultivate the sarcocarpus seedlings by using the rooting medium to obtain the rooted sarcocarpa seedlings. The rooting medium contains MS, 0.8 mg / L indole butyric acid and 0.3 g / L activated carbon. In the strong seedlin...

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PUM

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Abstract

The present invention discloses a Stauntonia obovatifoliola propagation method, which comprises: S1, making sterile explants of Stauntonia obovatifoliola; S2, culturing the sterile explants with an induced culture medium to obtain axillary buds; S3, culturing the axillary buds with a proliferation culture medium to obtain clustered shoots; S4, culturing the clustered shoots with a strong seedling culture medium to obtain Stauntonia obovatifoliola seedlings; S5, culturing the Stauntonia obovatifoliola seedlings with a rooting culture medium to obtain the rooted Stauntonia obovatifoliola seedlings, wherein the rooting culture medium contains MS, 0.3-1.3 mg / L of indole butyric acid and 0.1-0.5 g / L of active carbon; and S6, transplanting the rooted Stauntonia obovatifoliola seedlings into a matrix to culture. According to the present invention, with the tissue culture and the matrix cultivation, the wild plant Stauntonia obovatifoliola is successfully cultivated; and the reaction conditions such as the temperature, the time and the light intensity can be regulated, the obtained Stauntonia obovatifoliola has the high survival rate, the scale production of the Stauntonia obovatifoliola is solved, and the cultivation time of the Stauntonia obovatifoliola is shortened.

Description

technical field [0001] The present invention relates to a method for propagating Wuzhinateng, more specifically, the present invention relates to a method for rapidly propagating Wuzhinateng by using tissue culture. Background technique [0002] Wuzhi Nateng, commonly known as Udoba Nateng, Niu Teng, Akebia Aescine, etc., is the dry cane of the plant Akebia genus Udoba. Wuzhi Nateng has the effects of dispelling wind and relieving pain, relaxing tendons and activating collaterals, reducing swelling and dispelling toxins, dissipating heat and diuresis. For rheumatic bone pain, trigeminal neuralgia, sciatica, stomach pain, postoperative pain, bruises, edema, acute nephritis, hematuria and breast hyperplasia. Wuzhina Fujiwara plants are mainly distributed in forest margins or shrubs at the foot of mountains in Guangxi, Guangdong, Fujian, Jiangxi, Zhejiang and other provinces. In recent years, with the continuous increase of market demand, the collection of wild resources has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈乾平潘丽梅白隆华谷筱玉莫长明
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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