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Complementary deoxyribonucleic acid (cDNA) of insulin-like peptide (ILP)-2 gene in harmonia axyridis, and application of cDNA of ILP-2 gene in harmonia axyridis

A technology for insulin-like and lady beetles is applied in the fields of insect physiology and molecular biology, which can solve the problem of not involving the application of insulin-like peptides, and achieve the effects of prolonging the spawning period and increasing the amount of spawned eggs.

Inactive Publication Date: 2018-11-23
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research work on insect insulin-like peptides is still in its infancy, and the relevant reports only discuss the physiological functions of insulin-like peptides, and do not involve the application of insulin-like peptides in insect feeding

Method used

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  • Complementary deoxyribonucleic acid (cDNA) of insulin-like peptide (ILP)-2 gene in harmonia axyridis, and application of cDNA of ILP-2 gene in harmonia axyridis
  • Complementary deoxyribonucleic acid (cDNA) of insulin-like peptide (ILP)-2 gene in harmonia axyridis, and application of cDNA of ILP-2 gene in harmonia axyridis

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Embodiment Construction

[0014] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0015] The present invention clones the insulin-like peptide-2 gene cDNA from the heterochromia, which has the sequence shown in SEQ ID NO.1:

[0016] (1) Information of SEQ ID NO.1

[0017] (a) Sequential features

[0018] Sequence length: 366 base pairs

[0019] Type: nucleic acid

[0020] Chain type: double chain

[0021] Topology: Linear

[0022] (b) Molecular type: cDNA

[0023] (c) Assumption: No

[0024] (d) Antisense: no

[0025] (e) Original source: Harmonia axyridis

[0026] (f) Sequence description: SEQ ID NO.1

[0027] (2) Information of SEQ ID NO.2

[0028] (a) Sequential features

[0029] Sequence length: 121 amino acids

[0030] Type: amino acid

[0031] Chain type: single chain

[0032] Topology: Linear

[0033] (b) Molecule type: protein

[0034...

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Abstract

Insulin-like peptides (ILPs) in insects are widely involved in the regulation of various physiological processes such as growth, development and reproduction of the insects. Harmonia axyridis is an important predatory natural enemy insect. The invention discloses complementary deoxyribonucleic acid (cDNA) of an insulin-like peptide (ILP)-2 gene in the harmonia axyridis. After the eukaryotic expression of ILP-2 of the harmonia axyridis is realized, and the female adult of the harmonia axyridis is fed with the ILP-2, the egg production is increased by 20.2%. The ILP-2 of the harmonia axyridis can be used for the regulation of physiological processes such as spawning and diapause of the harmonia axyridis or other insects, and has a very broad application prospect in the large-scale productionof the insects.

Description

technical field [0001] The invention belongs to the field of insect physiology and molecular biology, and specifically relates to a heterochromia insulin-like peptide-2 gene cDNA and application thereof. Background technique [0002] The heterochromia is a predatory natural enemy of aphids, scale insects, psyllids, eggs and larvae of moths, etc., and belongs to the order Coleoptera. Due to the long life of adults and large amount of food, they can immediately control the target pests after release, and as the adults lay eggs and the larvae hatch, their control effect becomes stronger and stronger, so it is a kind of insecticide with broad development and utilization prospects. Natural enemy insects. [0003] Vertebrate insulin (Insulin) plays an important role in the process of glucose metabolism and has been widely used in the treatment of diabetes. Insects can also synthesize protein hormones similar to vertebrate insulin, called insulin-like peptides (Insulin-like Pepti...

Claims

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Application Information

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IPC IPC(8): C12N15/17C07K14/62A01K67/033
CPCA01K67/033C07K14/62
Inventor 毛建军冯彦姣
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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