Acute liver injury inducer and application thereof

A technology of acute liver injury and inducer, which is applied in the modeling field of severe acute liver injury model, can solve the problem that the same model of liver injury and liver function damage cannot objectively reflect liver injury, etc. The effect of short mold cycle

Inactive Publication Date: 2018-11-30
AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the complexity of liver injury and the diversity of liver function damage make the same model unable to reflect the liver injury comprehensively and objectively in essence. The above liver

Method used

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  • Acute liver injury inducer and application thereof
  • Acute liver injury inducer and application thereof
  • Acute liver injury inducer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Extract carbon tetrachloride and olive oil according to the volume ratio of 1:4 to prepare a mixed solution with a total volume of 5ml, filter bacteria with 0.22 micron bacteria filter respectively, store at room temperature for later use;

[0025] 2) Select clean Kunming mice of the same sex with a body weight of 25±2g, and randomly assign a group of 8 as a model group;

[0026] 3) The model group was intraperitoneally injected with carbon tetrachloride olive oil mixed solution, the injection dosage was 10mL / kg, fasting for 12 hours before administration, and the administration frequency was 1 time;

[0027] 4) 12 to 48 hours after the injection of the drug, according to the activity response of the mice; after removing the eyeballs and draining the blood, the above mice were killed by cervical dislocation, and about 100 mg of fresh liver tissue pieces were fixed in formalin, and further made into pathological sections. Hematoxylin-eosin staining (H&E staining) was ...

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Abstract

The invention provides an acute liver injury inducer and application of the acute liver injury inducer to establishment of a severe model mouse, and belongs to the field of model making of animal models. The acute liver injury inducer is a mixed solution comprising carbon tetrachloride and olive oil. The application is that the acute liver injury inducer is injected into a clean mouse abdominal cavity, and the acute liver injury severe model mouse is obtained after 12-48 h. According to a model making method, the model making success rate is high, condition requirements are low, the method issimple and convenient, the model making period is short, repeatability is good, the liver injury is obviously changed, demands for a liver experiment are sufficiently met, a target tool is provided for a mechanism researching the liver injury, the acute liver injury severe model mouse is particularly suitable for researching the pathogenesis of the differential liver injury, meanwhile a good toolis provided for screening of liver-injury-resistant drugs, and the acute liver injury severe model mouse can be used for efficacy observation.

Description

technical field [0001] The invention belongs to the field of modeling of animal models, and in particular relates to a modeling method of a severe acute liver injury model. Background technique [0002] Severe liver injury is a clinical syndrome of severe liver function injury characterized by a large number of liver cell degeneration, necrosis, and inflammatory cell infiltration caused by various etiologies in a short period of time, with high mortality. Common causes in my country include HBV (hepatitis B virus) infection, drug poisoning, etc., and its pathogenesis is still unclear. [0003] The acute liver injury model in experimental animals is an indispensable tool in the study of the pathogenesis of various liver diseases and the screening of therapeutic drugs. Although the corresponding progress has been made in liver injury models at home and abroad in recent years, there are many ways to create experimental liver injury animal models, mainly including two types: ch...

Claims

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Application Information

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IPC IPC(8): A61K36/32A61K31/02
CPCA61K9/0019A61K31/02A61K36/32A61K2300/00
Inventor 何毅怀王君黄文鸽沈访汪杰田仁冬
Owner AFFILIATED HOSPITAL OF ZUNYI MEDICAL COLLEGE
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