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Method for assisting in identifying wheat canopy temperature characteristics and special primer group thereof

A technology of wheat and specific primers, applied in biochemical equipment and methods, recombinant DNA technology, determination/inspection of microorganisms, etc., can solve the problems of canopy temperature vegetation coverage index, high technical requirements and deviation in the determination process.

Active Publication Date: 2018-12-11
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physiological traits of drought resistance are easily affected by the environment, and the determination process requires high technology
For example, the temperature of the canopy is greatly affected by the planting density and vegetation coverage index, and the measurement may cause a deviation of more than 1°C due to different infrared projection angles.

Method used

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  • Method for assisting in identifying wheat canopy temperature characteristics and special primer group thereof
  • Method for assisting in identifying wheat canopy temperature characteristics and special primer group thereof
  • Method for assisting in identifying wheat canopy temperature characteristics and special primer group thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Example 1. Acquisition of the SNP marker AX94392210 of genes related to wheat canopy temperature

[0075] 1. Acquisition of DH groups

[0076] Zhongmai 895 is a semi-winter multi-ear mid-late maturing variety bred by the Crop Science Institute of the Chinese Academy of Agricultural Sciences and the Cotton Research Institute of the Chinese Academy of Agricultural Sciences with Zhoumai 16 as the female parent and Liken 4 as the male parent. Long functional period, strong tillering ability, fast filling speed and other characteristics. In September 2009, it passed the examination and approval of the southern area of ​​Huanghuai wheat area. During the three-year variety comparison test and field demonstration from 2013 to 2015, Zhongmai 895 showed the characteristics of high yield, wide adaptability, disease and lodging resistance, and high temperature resistance in the late stage of grain filling.

[0077] Yangmai 16 is the variety with the largest planting area in the m...

Embodiment 2

[0103] Embodiment 2, establishment of method

[0104] 1. Extract the genomic DNA of the leaves of the wheat to be tested, and dilute to obtain a template solution. The DNA concentration in the template solution was about 20 ng / μL.

[0105] 2. Carry out KASP.

[0106] Primer working solution: take primer A, primer B and primer C, make up to 100 μL with sterile ultrapure water, and mix well. In the primer working solution, the concentrations of primer A, primer B and primer C are all 100 μM.

[0107] KASP 2×Master Mix is ​​a product of LGC (article number KBS-1016-002). KASP 2×Master Mix contains fluorescent probe A, fluorescent probe B, quenching probe A, quenching probe B, high-fidelity Taq enzyme, dNTP, Mg 2+ Wait. The sequence of fluorescent probe A is 5'-GAAGGTGACCAAGTTCATGCT-3', and the 5' end is connected with a FAM fluorescent group. The sequence of fluorescent probe B is 5'-GAAGGTCGGAGTCAACGGATT-3', and the 5' end is connected with HEX fluorescent group. The sequ...

Embodiment 3

[0120] Embodiment 3, application KASP primer set detects the family in the DH population

[0121] Wheat to be tested: 14 families in the DH population obtained in Example 1.

[0122] 1. Detect the genotype of the wheat to be tested based on the specific SNP, the method is the same as in Example 2.

[0123] 2. Detect the canopy temperature of the wheat to be measured.

[0124] The results are shown in Table 1.

[0125] Table 1 Segregation of AX94392210 marker allelic variation in Yangmai 16 / Zhongmai 895DH population

[0126]

[0127] The canopy temperature of CC genotype wheat was lower than that of AC / AA genotype wheat.

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Abstract

The invention discloses a method for assisting in identifying wheat canopy temperature characteristics and a special primer group thereof. The invention provides the specific primer group, wherein theprimer group is composed of a primer A as shown in a sequence 1, a primer B as shown in a sequence 2 and a primer C as shown in a sequence 3. The invention further discloses the method for identifying the canopy temperature characteristics of to-be-detected wheat; the method comprises the following steps that genotypes, based on specific SNP, of the to-be-detected wheat are detected; a canopy temperature of the wheat with a CC genotype is lower than that of the wheat with an AA genotype. The invention further discloses the method for identifying the canopy temperature characteristics of to-be-detected wheat; the method comprises the following steps that genome DNA of the to-be-detected wheat is used as a template, the primer group is adopted for KASP, fluorescence scanning is conducted todetermine genotypes, based on the specific SNP, of the to-be-detected wheat, and the canopy temperature of the wheat with the CC genotype is lower than that of the wheat with the AA genotype. The method for assisting in identifying the wheat canopy temperature characteristics and the special primer group thereof can be used for screening wheat with good canopy temperature characteristics and playan important role in culturing drought-resistance wheat varieties.

Description

technical field [0001] The invention relates to a method for assisting identification of wheat canopy temperature traits and a special primer set thereof. Background technique [0002] Canopy temperature, vegetation cover index and chlorophyll content are important physiological traits related to drought resistance in wheat. The canopy temperature refers to the average surface temperature of stems, leaves and other organs at different heights of the crop canopy, which is jointly regulated by various traits such as stomatal conductance, vascular bundle strength, and root depth, reflecting the overall water status of the plant. Under drought stress, the crop canopy absorbs solar radiation, part of which is used for photochemical reactions in photosynthesis, part of which is used for radiative fluorescence, and the rest is converted into heat energy, which increases the temperature of the plant canopy. The leaves of persistent green wheat can use more solar radiation energy fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/6895
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 肖永贵杨梦娇武玉莹何中虎夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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