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Rapid field detection method for amoxicillin antibiotics in water

A technology of amoxicillin and antibiotics, which is applied in the field of rapid detection of amoxicillin antibiotics in water bodies, can solve the problem of inability to realize rapid and sensitive detection of antibiotics at the same time, and achieve the effects of repeated use, rapid and efficient carrying, and improved sensitivity.

Active Publication Date: 2018-12-11
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reusable SERS substrates reported so far are based on random metallic particles or disordered arrangement of particles
It is impossible to achieve rapid and sensitive detection of antibiotics, improve the stability of the substrate and reuse the substrate at the same time
Therefore, the development of substrates that greatly enhance the SERS signal of the analyte and have high stability and repeatability needs to be further improved.

Method used

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  • Rapid field detection method for amoxicillin antibiotics in water
  • Rapid field detection method for amoxicillin antibiotics in water
  • Rapid field detection method for amoxicillin antibiotics in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Ag / Cu-BTC composite material detects amoxicillin

[0028] (1) Electrochemical synthesis of Cu-BTC metal-organic framework:

[0029] Use an electrochemical workstation, use a three-electrode system, the electrode is a screen-printed electrode, and use the i-t method to take 2mL50mmoL / L Cu(NO 3 ) 2 ·3H 2 O, 3.5mL 20mmol / L H 3 BTC, 3.5mL 20mmol / L Et 3 NHCl was added into the electrolytic cell, and the electrode was a screen-printed electrode, and a voltage of -1.3V was applied, and the electrochemical reaction was performed for 20 minutes. The electrode was taken out and dried at 50°C for 30 min to obtain the Cu-BTC metal-organic framework ( figure 1 ).

[0030] (2) Synthesis of silver nanoparticles by in situ growth method:

[0031] Add 10 μL of 20mmoL / L 3-aminopropyltrihydroxysilane dropwise on the Cu-BTC metal-organic framework synthesized in the above step (1), let it stand at room temperature (25°C-28°C) for 5min, and then place the Cu-BTC The BT...

Embodiment 2

[0035] Embodiment 2: detection of amoxicillin in lake water

[0036] figure 1 The flow chart of amoxicillin in the water body of the embodiment of the present invention is schematically provided, and the detection method comprises the following steps:

[0037] (1) Cu-BTC metal-organic framework is synthesized by electrochemical method, and the steps are the same as in Example 1

[0038](2) Synthesis of silver nanoparticles by in-situ growth method, the steps are the same as those in Example 1

[0039] (3) SERS technology to detect amoxicillin in water

[0040] The water used in the laboratory was taken from the lake water of the school, and 10 μL of lake water was added dropwise to the screen-printed electrode of the Ag / Cu-BTC composite material prepared above, and the Raman signal was detected by a portable Raman spectrometer. The excitation wavelength was 785nm, the integration time is 10s, the SERS spectrum of the lake water is obtained and compared with the standard cur...

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Abstract

The invention discloses a rapid field detection method for amoxicillin antibiotics in water. The method comprises the following steps: (1) synthesizing a copper metal organic framework Cu-MOFs on thesurface of a screen-printed electrode by an electrochemical method; (2) synthesizing an Ag / Cu-BTC composite nanometer material on the surface of the Cu-MOFs by virtue of an in-situ growth method; (3)enriching amoxicillin antibiotics in to-be-detected water on the Ag / Cu-BTC; and (4) detecting the enriched amoxicillin by utilizing a portable Raman spectrometer, and performing qualitative and quantitative analytical detection on the amoxicillin in the to-be-detected water. The method disclosed by the invention has the characteristics of being rapid in analysis, high in sensitivity, small in sample usage amount, wide in application range, simple and convenient to operate, convenient to carry and the like; and the SERS (Surface Enhanced Raman Scattering) substrate prepared by the method disclosed by the invention has controllable morphology and many SERS active hot spots, can realize analytical detection of the amoxicillin antibiotics in the water, and has a detection limit of 1.8*10<-10>M.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman spectroscopic analysis, and in particular relates to a method for on-site rapid detection of amoxicillin antibiotics in water bodies. Background technique [0002] Antibiotics are frequently used in the treatment of human and animal diseases, however, antibiotic residues in aquatic environments such as wastewater, groundwater, surface water, and even drinking water cause adverse health effects. Therefore, there is an increasing need for the development of efficient analytical methods for the rapid and sensitive identification and quantification of antibiotics in water. Conventional antibiotic detection strategies are chromatography and enzyme-linked immunosorbent assay (ELISA). In recent years, the Chinese patent (publication number CN 107589212A) adopts high performance liquid chromatography to analyze and detect amoxicillin. Although the detection result has high accuracy, the ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 李丹段化珍邓维曹秀开张钦梅
Owner SHANGHAI INST OF TECH
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