A glass nanopore with a diameter less than 10nm, its preparation method and its application for detecting dna

A nanopore and glass technology, applied in the field of preparation and detection of DNA, in the field of glass nanopores, can solve the problems of irreversibility and high experimental cost, preventing practical use, inapplicability, etc., to improve the DNA transport signal and signal-to-noise ratio. , the effect of reducing the concentration, the method is simple
CN108996461BActive Publication Date: 2020-06-02ANHUI NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI NORMAL UNIV
Publication Date
2020-06-02

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Abstract

The invention provides a glass nanopore with a diameter less than 10nm, a preparation method and an application thereof for detecting DNA, directly optimizing a drawing parameter and a drawing processof a laser drawing device, and reducing the diameter of the conical glass capillary nanopore. By this method, glass micropore nanopores smaller than 10 nm in diameter can be easily obtained. The method is simple, direct and environmentally friendly, and does not rely on expensive vacuum equipment. The shrinkage of the glass capillary nanopore prepared by the invention not only reduces the concentration of detectable DNA, but also remarkably improves the DNA transporting signal and the signal-to-noise ratio, so that the method and the glass nanopore are expected to be used for DNA transportingresearch.
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Description

technical field

[0001] The invention belongs to the field of nanomaterial preparation, and in particular relates to a glass nanopore with a diameter less than 10 nm, a preparation method and an application for detecting DNA. Background technique

[0002] With the rapid development of life sciences, the research on life phenomena has gone deep to the level of single molecule and single cell. For this reason, analytical means having detection sensitivity at the single-molecule level are required to adapt to this development. Among many single-molecule detection technologies, the nanopore single-molecule detection technology based on the Coulter counter principle is simple and accurate, and shows great application potential in fast and cheap DNA detection, and has attracted increasing attention.

[0003] Although biological nanopores were first studied and applied to detect DNA, people also found its shortcomings during the research process. For example, due to the poor stabi...

Claims

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