Tandem mass spectrum detection kit capable of simultaneously measuring 16 types of bile acid, and application thereof

A detection kit and tandem mass spectrometry technology, which is applied in the field of tandem mass spectrometry detection kits, can solve the problems of decreased reactivity, unsatisfactory reagent stability, and short reagent stability time, achieving high sensitivity, good specificity, and wide application value effect

Inactive Publication Date: 2018-12-18
易达精准(杭州)科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagent provided in this invention has a short stable time when the lid is opened and used at 2-8°C; in the process of airtight and light-proof storage at 2-8°C, the stability of the reagent is not ideal. Prolonged, the responsiveness will decrease

Method used

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  • Tandem mass spectrum detection kit capable of simultaneously measuring 16 types of bile acid, and application thereof
  • Tandem mass spectrum detection kit capable of simultaneously measuring 16 types of bile acid, and application thereof
  • Tandem mass spectrum detection kit capable of simultaneously measuring 16 types of bile acid, and application thereof

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Experimental program
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Effect test

preparation example Construction

[0065] 4. Preparation of dry matrix standard and quality control

[0066] Dry matrix standard: After preparing the standard working solution according to the above method, take 50uL of each standard working solution and drop it on Whatman 903 filter paper, and let it dry naturally at room temperature for 2 hours.

[0067] Dry matrix quality control sample: After preparing the quality control sample solution according to the above method, take 50uL of each quality control sample solution and drop it on whatman 903 filter paper, and let it dry naturally at room temperature for 2 hours.

[0068] 5. The 16 kinds of bile acids involved in the present invention have large individual differences, and the linear range of the working curve can be adjusted according to the actual situation. According to the content of 16 kinds of bile acids in the human body, the 16 kinds of bile acids can be divided into three groups with different concentration levels to prepare standard working solut...

Embodiment 1

[0106] The test kit includes:

[0107]

[0108] The precipitating agent is methanol, the diluent is 80% methanol aqueous solution, the mobile phase A is 0.1% formic acid aqueous solution, and the mobile phase B is acetonitrile.

Embodiment 2

[0110] Precipitating agent is acetonitrile, diluent is 50% volume fraction of acetonitrile aqueous solution, mobile phase A is 5mmol / L ammonium acetate-0.1% acetic acid aqueous solution, mobile phase B is acetonitrile.

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Abstract

The invention discloses a tandem mass spectrum detection kit capable of simultaneously measuring 16 types of bile acid. The kit comprises a standard substance, a quality control substance, an interiorlabel substance, precipitant, diluent and a flowing phase, wherein the precipitant is at least one of methyl alcohol, ethyl alcohol and acetonitrile; the diluent consists of water, methyl alcohol, ethyl alcohol and acetonitrile, wherein the volume ratio of water, methyl alcohol, ethyl alcohol to acetonitrile is (1-50):(0-90):(0-50):(0-50), and methyl alcohol, ethyl alcohol and acetonitrile are not zero at the same time; and the flowing phase comprises a flowing phase A and a flowing phase B, wherein the flowing phase A is the aqueous solution of ammonium formate or ammonium acetate-methanoicacid or acetic acid, and the flowing phase B is methyl alcohol and/ or acetonitrile. The invention also discloses the application of the tandem mass spectrum detection kit. The tandem mass spectrum detection kit disclosed by the invention has the characteristics of high sensitivity, good specificity and the like, can accurately, simply and quickly simultaneously detect 16 types of bile acid in a matrix, and has a high application value.

Description

technical field [0001] The invention relates to the field of clinical detection, in particular to a tandem mass spectrometry detection kit for simultaneously measuring 16 bile acids and an application thereof. Background technique [0002] Bile acids are the products of cholesterol degradation and metabolism in the liver. Through the enterohepatic circulation of bile acids, the body can effectively use the limited bile acids synthesized by the liver. Bile acids can be divided into two categories according to their structure, one is free bile acids, including cholic acid (CA), deoxycholic acid (DCA), chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA) ) and lithocholic acid (LCA), the other is the combined product of free bile acids and glycine or taurine, which are called glycine-bound bile acids and taurine-bound bile acids, respectively. [0003] With the progress of research, people have a deeper understanding of the metabolic regulation of bile acids and their phy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 李士敏袁京群吴筱丹葛志伟朱亚尔李家睿
Owner 易达精准(杭州)科技有限公司
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