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Method for calculating ratio of measurement object substance to comparison object substance, program, storage medium, and device

A technology for measuring objects and ratios, which can be used in measurement devices, biological testing, biological material analysis, etc., and can solve problems such as inability to correctly reflect blood sugar status.

Inactive Publication Date: 2018-12-21
EIKEN KAGAKU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since Hb is glycosylated in a manner that depends on the level of blood sugar concentration, unless a value showing the relationship between the HbA1c concentration and the total Hb concentration is used, the total Hb concentration will not be correct even if expressed as the HbA1c concentration because the total Hb concentration is unknown reflect blood sugar status

Method used

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  • Method for calculating ratio of measurement object substance to comparison object substance, program, storage medium, and device
  • Method for calculating ratio of measurement object substance to comparison object substance, program, storage medium, and device
  • Method for calculating ratio of measurement object substance to comparison object substance, program, storage medium, and device

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no. 1 approach

[0095] Figure 1A Shown is an exemplary procedure from immunoreaction to absorbance measurement. When adding a sample as an antigen to a Figure 1A (a) When the antibody in the state shown in (a) is bound to the carrier, it will occur Figure 1A (b) Antigen-antibody responses shown. after that, in Figure 1A In the state of the immunoagglutination reaction shown in (c), carry out Figure 1A (d) Absorbance measurements indicated. The concentration of HbA1c can also be obtained by such common antigen-antibody reaction and absorbance measurement. In addition, although an example in which two antibodies supported on a carrier react with one antigen is shown in the figure, the antigen and the antibody can also be interchanged.

[0096] Hereinafter, a method for obtaining the ratio of HbA1c to the total Hb will be described.

[0097] Figure 1B It is a schematic diagram of the HbA1c concentration measurement technology according to the first embodiment of the present invention, ...

no. 2 approach

[0134] Hereinafter, a second embodiment of the present invention will be described in detail.

[0135] In the first embodiment, if Figure 5 As shown, a separate calibration curve needs to be set up for each of all total Hb concentrations, which makes the process for determining the HbA1c concentration very cumbersome.

[0136] Hereinafter, a processing method for solving the above-mentioned problem by substituting for step S5 in the first embodiment will be described in detail.

[0137] In this embodiment, based on the absorbance change (variation Δ), such as 525nm wavelength (see figure 2 ) under the method of absorbance change with time is described.

[0138] As described above in the first embodiment, Figure 5 It is a graph plotted against the HbA1c concentration and the change in absorbance at a wavelength of 525 nm (change amount Δ) 5 minutes after the start of the reaction. from Figure 5 As can be seen in , the change in absorbance correlates with the ratio of H...

no. 3 approach

[0187] Hereinafter, a third embodiment of the present invention will be described in detail.

[0188] In the present embodiment, the absorbance ratio (absorbance at 525 nm / absorbance at 630 nm) is used as an index of absorbance change and used as an independent variable to obtain a calibration curve for obtaining the HbA1c concentration.

[0189] Figure 14 For the determination of samples with a total Hb concentration of 0.5mg / mL, 0.75mg / mL, 1.0mg / mL, 1.5mg / mL, 2.0mg / mL, and 2.5mg / mL and a HbA1c ratio of 3.7 to 15.3% of the total Hb 5 minutes after the reaction started, the ratio of HbA1c to total Hb and the ratio of the absorbance at 525nm wavelength to the absorbance at 630nm wavelength, that is, the absorbance ratio (Ratio) was plotted.

[0190] first, Figure 14 The horizontal axis represents the proportion of HbA1c to total Hb, from Figure 14 It can be seen that the absorbance ratio (Ratio) corresponding to the ratio of HbA1c to the total Hb is roughly inversely prop...

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Abstract

A method for calculating the ratio of a measurement object substance to a comparison object substance using a measurement reagent of the measurement object substance, whereby the ratio of the measurement object substance to the comparison object substance can easily be calculated from a measured value of light absorbance using a single calibration curve (multiplex calibration curve) that is not dependent upon concentration of the comparison object substance.

Description

technical field [0001] The invention relates to a technique for determining the proportion of analytes in contrast objects. Background technique [0002] In the sample test of clinical examination, the patient's blood, cerebrospinal fluid, urine, feces, tissue or cells, etc. are collected as the sample to be tested, and the proteins, enzymes, electrolytes, metal ions, lipids, carbohydrates, etc. , Vitamins and other chemical substances are measured to understand, for example, organ abnormalities and infections caused by bacteria or viruses. Therefore, sample testing can be used as a means to diagnose diseases, determine treatment options, and obtain indicators of treatment effects. [0003] In order to make the measured value of each chemical substance in the sample as the analyte relative to the reference value reflect the abnormality of the corresponding organ to be tested, the measured value can be expressed in terms of concentration, such as mg / dL or mol / L . In additi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/543G01N33/5002G01N33/66G01N33/72G01N21/314G01N33/536G01N33/553G01N33/723G01N2021/3155G01N2333/765G01N33/53G06F17/18
Inventor 安藤沙智子吉野学
Owner EIKEN KAGAKU
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