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Preserving fluid as well as preparation method and application thereof

A preservation method and technology of preservation solution, applied in the field of cell preservation solution, can solve the problems of easily broken and degraded, unable to be preserved for a long time, unable to effectively protect CTC form and antigen expression, etc., to improve repeatability and reliability, protect form and The effect of antigenic expression

Inactive Publication Date: 2018-12-28
广州维帝医疗技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are still some problems in the existing cell preservation solution, such as the need for low temperature preservation, but low temperature preservation puts forward higher requirements on the stability and transportation conditions of the sample, or it cannot be preserved for a long time at room temperature, making the collected CTC cells It is easily broken and degraded within a few hours, cannot effectively protect the morphology and antigen expression of CTC, and cannot guarantee the repeatability and reliability of CTC analysis and detection, which is not conducive to subsequent analysis and detection

Method used

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  • Preserving fluid as well as preparation method and application thereof
  • Preserving fluid as well as preparation method and application thereof
  • Preserving fluid as well as preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0088] 1. A cell preservation solution, comprising a cell protection agent, a nuclease inhibitor, a chelation stabilizer and a metabolism inhibitor.

[0089] Specifically, the parts by weight of each added component per 100 parts by volume of the cell preservation solution are shown in Table 1, wherein the unit ratio of parts by weight to parts by volume is g / mL.

[0090] Table 1 Example 1 The parts by weight of each added component per 100 parts by volume of the cell preservation solution

[0091]

[0092] Finally, the volume of the above mixture was adjusted to 100 parts by volume to obtain a cell preservation solution.

[0093] 2. A cell preservation method

[0094] Add the above cell preservation solution into the blood, wherein the volume ratio of the cell preservation solution to blood is 3:100.

[0095] After the cell preservation solution is added to the blood, the content of the cell protection agent in the blood is 5mg / mL, the content of the nuclease inhibitor i...

Embodiment 2-5

[0097] The difference between Examples 2-5 and Example 1 lies in the content of the chelation stabilizer EDTA-K3 in the blood, as shown in Table 2 specifically.

[0098] The content of chelating stabilizer EDTA-K3 in the blood of table 2 embodiment 2-5

[0099]

Embodiment 6-9

[0101] The difference between Examples 6-9 and Example 1 lies in the content of the metabolism inhibitor glyceraldehyde in the blood, as shown in Table 3 specifically.

[0102] The content of metabolism inhibitor glyceraldehyde in the blood of table 3 embodiment 6-9

[0103]

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Abstract

The invention provides a cell preserving fluid, a preparation method and an application thereof and a cell preserving method and belongs to the technical field of cell preserving fluids. The cell preserving fluid comprises a cell protective agent, a nuclease inhibitor, a chelation stabilizer and a metabolism inhibitor. Under the synergistic effect of the four components including the cell protective agent, the nuclease inhibitor, the chelation stabilizer and the metabolism inhibitor, the obtained cell preserving fluid can effectively preserve CTC cells at the normal temperature for a long time, effectively protect the form and antigen state of the CTC cells, allow long-distance transportation and improve repeatability and reliability of CTC analysis and detection.

Description

technical field [0001] The invention belongs to the technical field of cell preservation solution, and in particular relates to a cell preservation solution, a preparation method and application thereof, and a cell preservation method. Background technique [0002] Circulating tumor cells (circulating tumor cells) are referred to as CTCs. CTCs are tumor cells free in the blood of patients, carrying all the genes of the tumor, and can be used as samples for genetic testing. The detection of circulating tumor cells can be effectively applied to early diagnosis in vitro, rapid evaluation of chemotherapy drugs, can reflect the genetic status of the tumor in real time, clearly guide the patient's next drug selection, and improve the treatment effect. Individualized treatment includes clinical drug screening, Detection of drug resistance, monitoring of tumor recurrence and development of new tumor drugs, etc. [0003] However, there are still some problems in the existing cell pr...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 赖晓冬邓昕
Owner 广州维帝医疗技术有限公司
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