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Preparation method and application of an electrochemical immunosensor for detecting Alzheimer's disease markers

An Alzheimer's disease, immunosensor technology, applied in the field of immunoassay and biosensing, can solve the problems of cumbersome detection methods, high detection limit, high cost, etc., to promote electron transfer, lower detection limit, improve electrical conductivity sexual effect

Inactive Publication Date: 2020-01-14
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many detection methods for Alzheimer's disease markers, such as radioimmunoassay, immunoradiometry, electrochemiluminescence analysis, etc., but most of the detection methods are cumbersome, complicated to operate, expensive, and have high detection limits. Therefore, it is of great significance to establish a fast, simple and sensitive detection method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 A preparation method of an electrochemical immunosensor for detecting Alzheimer's disease markers, the steps are as follows:

[0040] 1) Preparation of reduced graphene oxide functionalized with gold nanoparticles

[0041] Dissolve 5 mg of graphene oxide in 10 mL of ultrapure water, and after ultrasonication for 60 min, add 200 µL of 1% HAuCl 4 and 4 mL, 50 wt% thiogen solution, stirred at room temperature for 12 h, centrifuged and washed three times with ultrapure water, and the obtained solid was dried in a vacuum freeze dryer at 60 °C for 10 h to prepare gold nanoparticles functionalized redox Graphene;

[0042] 2) Ab 2 -AuCu x O@CeO 2 Preparation of hatching solution

[0043] Take 0.4 g of Ce(NO 3 ) 3 ·6H 2 Dissolve O in 1 mL of mixed solvent, add 15 mL of ethylene glycol, stir for 60 min, transfer the mixture to a 30 mL polytetrafluoroethylene reactor, heat to 150 °C, and react for 200 min; cool to room temperature, After centrifugation, the r...

Embodiment 2

[0055] Example 2 A preparation method of an electrochemical immunosensor for detecting Alzheimer's disease markers, the steps are as follows:

[0056] 1) Preparation of reduced graphene oxide functionalized with gold nanoparticles

[0057] Dissolve 10 mg of graphene oxide in 10 mL of ultrapure water, and after ultrasonication for 60 min, add 200 µL of 1% HAuCl 4 and 4 mL, 50 wt% Thiogen solution, stirred at room temperature for 12 h, centrifuged and washed three times with ultrapure water, and the obtained solid was dried in a vacuum freeze dryer at 60 °C for 12 h to prepare gold nanoparticles functionalized redox Graphene;

[0058] 2) Ab 2 -AuCu x O@CeO 2 Preparation of hatching solution

[0059] Take 0.5 g of Ce(NO 3 ) 3 ·6H 2 Dissolve O in 1 mL of mixed solvent, add 15 mL of ethylene glycol, stir for 60 min, transfer the mixture to a 30 mL polytetrafluoroethylene reactor, heat to 180 °C, and react for 200 min; cool to room temperature, After centrifugation, the res...

Embodiment 3

[0071] Example 3 A preparation method of an electrochemical immunosensor for detecting Alzheimer's disease markers, the steps are as follows:

[0072] 1) Preparation of reduced graphene oxide functionalized with gold nanoparticles

[0073] Dissolve 20 mg of graphene oxide in 10 mL of ultrapure water, and after ultrasonication for 60 min, add 200 µL of 1% HAuCl 4 and 4 mL, 50 wt% Thiogen solution, stirred at room temperature for 12 h, centrifuged and washed three times with ultrapure water, and dried in a vacuum freeze dryer at 60 °C for 14 h to prepare gold nanoparticles functionalized redox Graphene;

[0074] 2) Ab 2 -AuCu x O@CeO 2 Preparation of hatching solution

[0075] Take 0.6 g of Ce(NO 3 ) 3 ·6H 2 O was dissolved in 1 mL of mixed solvent, 15 mL of ethylene glycol was added, stirred for 60 min, the mixture was transferred to a 30 mL polytetrafluoroethylene reactor, heated to 200 °C, and reacted for 200 min; cooled to room temperature, After centrifugation, the...

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Abstract

The invention relates to a preparation method and application of an electrochemical immunosensor for detecting Alzheimer's disease markers, and belongs to the fields of novel nano functional materials, immune analysis and biosensors. The prepared gold nanoparticles functionalized reduced graphene oxide was used as the substrate material to increase the immobilization capacity of the Alzheimer's disease marker capture antibody and effectively accelerate the electron transfer rate on the electrode surface; the preparation of AuCu x O@CeO 2 Composite nanomaterials as signal amplification tags to immobilize detection antibodies; through Au NPs, CeO 2 with Cu x With the synergistic effect of O, the composite material further improves the catalytic efficiency; the prepared electrochemical immunosensor can be used for the detection of Alzheimer's disease-related markers. It has strong specificity, high sensitivity and low detection limit, and has important scientific significance and application value.

Description

technical field [0001] The invention belongs to the technical field of immune analysis and biosensing, and provides a preparation method and application of an electrochemical immunosensor for detecting Alzheimer's disease markers. Background technique [0002] Alzheimer's disease (AD) is a degenerative disease of the central nervous system characterized by progressive cognitive dysfunction and behavioral impairment, and is the most common type of senile dementia, accounting for about 50% of senile dementia %above. Clinical manifestations include memory impairment, aphasia, apraxia, agnosia, impairment of visual abilities, impairment of abstract thinking and calculation abilities, and changes in personality and behavior. The clinical symptoms of AD can be roughly divided into two aspects, one is cognitive decline and its accompanying life ability decline symptoms, the other is non-cognitive neuropsychiatric symptoms. The molecular weight of β-amyloid (Aβ) is about 4kDa, whi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/26
CPCG01N27/26G01N27/308G01N27/3271G01N27/3272G01N27/3275
Inventor 李月云高增强张春燕张栓李新进徐振刘青王平陈志伟董云会
Owner SHANDONG UNIV OF TECH
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