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A preparation method of iron oxide nanoparticles and its application in tumor targeted diagnosis and treatment

A technology of iron oxide nanoparticles and particles, which is applied in the field of medical anti-tumor drugs, can solve problems such as short half-life, radiotoxicity, and inapplicability to clinical high-throughput testing requirements, and achieve strong tissue penetration, improved sensitivity and specificity, Effects of efficient treatment and simultaneous imaging

Active Publication Date: 2021-08-24
ZHENJIANG NO 1 PEOPLES HOSPITAL
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Problems solved by technology

Although this method is sensitive and quantitative, 18F-FDG is not suitable for clinical high-throughput testing requirements due to its short half-life of the isotope.
In addition, radiotracers often have radiotoxicity problems for body organs

Method used

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  • A preparation method of iron oxide nanoparticles and its application in tumor targeted diagnosis and treatment
  • A preparation method of iron oxide nanoparticles and its application in tumor targeted diagnosis and treatment
  • A preparation method of iron oxide nanoparticles and its application in tumor targeted diagnosis and treatment

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Embodiment

[0031] A kind of preparation method of novel iron oxide nanoparticle provided by the invention, carry out concrete experiment according to the following steps:

[0032] Step 1: Take 25.3mg of polylactic acid-polyethylene glycol (PLA-PEG), put it in 2mL of dimethylformamide (DMF), and then place the above mixture in 6mL of water for 3h;

[0033] Step 2: Add 550 mg of 1-3-dimethylaminopropyl-3-ethylcarbodiimide hydrochloride (EDC HCl) and 370 mg of N-hydroxysuccinimide (NHS) to the above step After the reaction system of 1, stir for 20min;

[0034] Step 3: After adding 350mg of glucosamine hydrochloride (DGH) to the reaction system in step 2, stir for 12h;

[0035] Step 4: Dialyze and purify the reaction system obtained in step 3 for 24 hours, then filter bacteria through a 0.22 μm filter membrane to obtain PLA-PEG-CO-NHC 6 h 11 o 5 ;

[0036] Step 5: After oleic acid (OA) was conjugated with paclitaxel (PTX), with PLA-PEG-CO-NHC 6 h 11 o 5 OA-PTX-OA-PLA-PEG-CO-NHC ​​mix...

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Abstract

The invention provides a method for preparing iron oxide nanoparticles: placing polylactic acid-polyethylene glycol and dimethylformamide in water; placing 1-3-dimethylaminopropyl-3-ethylcarbodiimide Hydrochloride and N-hydroxysuccinimide were added to the above reaction system; then glucosamine hydrochloride was added to it; then dialysis purification and filtration were performed; after oleic acid was conjugated with paclitaxel, it was mixed with PLA-PEG-CO-NHC 6 h 11 o 5 Mix in equimolar ratio; take Fe 3 o 4 Add ethanol to @OA NPs, use a magnet to adsorb and separate the system, then add tetrahydrofuran to it for ultrasonic oscillation; then add OA‑PTX‑OA‑OA‑PLA‑PEG‑CO‑NHC to it 6 h 11 o 5 Perform ultrasonic oscillation; then drop the obtained reaction system into water, perform ultrasonic oscillation, and then dialysis, purify and filter. The present invention also includes applying the nanoparticles prepared by any one of the above preparation methods to the targeted diagnosis and treatment of tumors. The iron oxide nanoparticles provided by the invention have dual functions of tumor treatment and tracer, high sensitivity, strong specificity, and strong penetrability.

Description

technical field [0001] The invention belongs to the field of medical antitumor drugs, and in particular relates to a preparation method of novel iron oxide nanoparticles and its application in targeted diagnosis and treatment of tumors. Background technique [0002] In the past few decades, various anticancer drugs have been widely used in the treatment of tumors. However, antitumor drugs cannot precisely target tumor cells in tumor pathological sites, thus weakening their anticancer effects and causing severe toxicity and side effects. Covalently linking anticancer drugs to ligands that can specifically target and bind tumor cells will increase the therapeutic effect of tumors. At the same time, the real-time in vivo evaluation of tumor treatment effect is of great significance for adjusting treatment plan and tumor prognosis. Existing technologies, such as fluorescence imaging, evaluate chemotherapy drugs, but the safety of the fluorescent reagent itself has problems, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/08A61K49/18A61K47/54A61K47/69A61K31/337A61K31/7008A61P35/00B82Y5/00B82Y15/00
CPCA61K31/337A61K31/7008A61K49/08A61K49/183A61K47/542A61K47/6935A61K47/6937A61P35/00B82Y5/00B82Y15/00A61K2300/00
Inventor 熊非单秀红顾宁钱炜柳迎昭
Owner ZHENJIANG NO 1 PEOPLES HOSPITAL
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