Injection pig uterus transfer factor as well as preparation method and application thereof

A technology of transfer factor and pig placenta, which is applied in the field of biomedicine, can solve the problem that pig placenta cannot obtain high-purity, high-yield transfer factor, etc., and achieve the effects of shortening the growth cycle, reducing production costs, and increasing the amount of extraction

Inactive Publication Date: 2019-01-08
派生特(福州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of this, in order to solve the problem that high-purity and high-yield transfer factor cannot be obtained from pig placenta as raw material, the present invention provides a preparation method of pig placenta transfer factor for injection, which has short time, controllable quality and high yield , suitable for large-scale industrial production

Method used

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  • Injection pig uterus transfer factor as well as preparation method and application thereof
  • Injection pig uterus transfer factor as well as preparation method and application thereof
  • Injection pig uterus transfer factor as well as preparation method and application thereof

Examples

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preparation example Construction

[0027] The invention provides a preparation method of pig placenta transfer factor for injection, comprising the steps of:

[0028] (1) pig placenta is homogenized to obtain pig placenta homogenate;

[0029] (2) Freezing and thawing the pig placenta homogenate repeatedly 5 to 7 times to obtain a freeze-thaw solution;

[0030] (3) mixing the freeze-thaw solution with a hydrochloric acid solution with a pH value of 2 to 4, stirring and extracting at 3 to 5° C. to obtain an extract;

[0031] (4) Carry out double-layer filtration to extract liquid, obtain separation liquid and precipitation;

[0032] (5) After the separation liquid is mixed with the chitosan, microfiltration and ultrafiltration are carried out successively to obtain the ultrafiltrate; the quality of the chitosan and the volume ratio of the separation liquid are 0.5 to 2:100;

[0033] (6) Adjust the pH value of the ultrafiltrate to 6.8-7.2, adjust the osmotic pressure to 280-320 mosm / kg, and sterilize to obtain t...

Embodiment 1

[0060] The first step: remove blood and serosa from 5 kg of fresh and healthy pig placenta, wash it with pure water for 3 times, and then wash it with sterilized injection for 2 times to obtain 4 kg of placenta cotyledon and meat port.

[0061] The second step: treating 4 kg of pig placenta to be used in the first step in deionized hot water at 60° C. for 3 to 5 seconds.

[0062] The third step: mash the pig placenta obtained in the second step to a particle size of 7-9 μm at a speed of 5000 r / min, and then homogenize the mashed material in a colloid mill to obtain 3.8 kg of a homogenate.

[0063] Step 4: Use 500 ml non-pyrogenic plastic bottles to store the homogenate liquid, a total of 8 bottles, freeze in liquid nitrogen for 24 hours, and thaw the liquid nitrogen frozen homogenate liquid with a power of 500w for 30 minutes.

[0064] Step 5: Repeat the steps of step 4, and freeze and thaw repeatedly 5 times.

[0065] Step 6: Mix the repeated freezing and thawing solution wi...

Embodiment 2

[0075] by Figure 4 The shown pig placenta extraction device carries out the extraction of pig placenta transfer factor, Figure 4 Among them, 1 is the tank body, 2 is the pressure gauge, 3 is the valve, 4 is the high-pressure air inlet, 5 is the 200 mesh filter, 6 is the 300 mesh filter, 7 is the separation liquid collection tank, 8 is the speed regulating pump, 9 10 is a filter element of 0.22 μm specification, 11 is a drain valve, 12 is a microfiltrate collection tank, 13 is an ultrafilter element of 6KD specification, and 14 is an ultrafiltrate collection tank.

[0076] The first step: 7 kg of fresh and healthy pig placenta, blood and serosa were removed, washed 5 times with pure water, and then washed 2 times with sterilized injection to obtain 5.5 kg of placenta cotyledon and meat port.

[0077] The second step: treating 5.5 kg of pig placenta to be used in the first step in deionized hot water at 80° C. for 3 to 5 seconds.

[0078] The third step: mash the pig placent...

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Abstract

The invention provides an injection pig uterus transfer factor and belongs to the technical field of biomedicines. A preparation method comprises the following steps: cleaning fresh pig uterus, and homogenizing so as to obtain pig uterus homogenate; repeatedly freezing and thawing the pig uterus homogenate for 5-7 times, mixing with a hydrochloric acid solution of which the pH value is 2-4, and stirring and extracting at 3-5 DEG C so as to obtain an extract liquid; carrying out double-layer filtration on the extract liquid so as to obtain a separation liquid, putting chitosan as a flocculant into the separation liquid, carrying out micro filtration and ultrafiltration in sequence so as to obtain an ultrafiltration liquid; adjusting the pH value and the osmotic pressure of the ultrafiltration liquid, and sterilizing, thereby obtaining the injection pig uterus transfer factor. By adopting the preparation method of the injection pig uterus transfer factor provided by the invention, activity of the injection pig uterus transfer factor can be well and completely maintained, the production cycle can be also shortened, the extraction amount of the transfer factor can be increased, the production cost can be reduced, and the transfer factor is applicable to large-scale industrial production. The injection pig uterus transfer factor can be applied to preparation of immunopotentiators and vaccine adjuvants.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a porcine placenta transfer factor for injection and a preparation method and application thereof. Background technique [0002] Transfer factor is a kind of small molecule dialyzable substance released by T lymphocytes with immune knowledge in animal protein cells. It is soluble in water, can induce the activation of immune cells in the body, enhance the immunity of the body, and can transfer the Cellular immunity to various antigens such as fungi, parasites, histocompatibility antigens and tumor antigens. Because the transfer factor is safe to use, acts quickly, and has no antigenicity, it can be used as an immune stimulating agent and an auxiliary preparation at the same time, which can significantly improve the prevention and control effect on animal diseases. [0003] At present, there are many spleen transfer factors in the market, such as pigs and cattle, but there ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/50A61K9/08A61P37/04A61K39/39
CPCA61K9/0019A61K9/08A61K35/50A61K39/39A61P37/04
Inventor 王晓斌刘友霖徐磊卢永恒
Owner 派生特(福州)生物科技有限公司
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