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A kind of Bacillus velei zsy-1 wettable powder, its preparation method and application

A technology of Bacillus velesi and ZSY-1, which is applied in the field of tomato gray mold and early blight disease control, can solve problems such as weak research, gaps in the number of active strains or plant numbers in field application research, and difficulties in chemical control.

Active Publication Date: 2021-04-20
TIANJIN AGRICULTURE COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in recent years, with the emergence of problems such as chemical pesticide residues, drug resistance and phytotoxicity, biological pesticides have become a new development direction, and have been extensively studied by plant protection workers, and it has been found that various plant extracts and biocontrol microorganisms are effective against Botrytis cinerea and Botrytis cinerea and Early blight disease has a good control effect, but currently these studies are mainly focused on indoor research and research on the control effect of strains or their active metabolites, and studies have found that some strains or active ingredients have significant differences in indoor and field application effects
In addition, there is a large gap between the number of active strains or plants and the number of preparation development and field application research, especially the research on the control of tomato botrytis and early blight by endophytic bacteria is still relatively weak
In addition, in the research on the biological control of tomato early blight and gray mold, tomato gray mold has been studied more, and tomato early blight, as a disease that mainly infects leaves, also has an important impact on tomato growth and development. , and with the discovery of drug resistance problems, it is difficult to control it chemically. Therefore, in the later research, the plant endophytic bacteria control tomato gray mold and early blight and the field screening and preparation processing of active strains for the purpose of production practice application And research on field control effects needs to be further strengthened

Method used

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  • A kind of Bacillus velei zsy-1 wettable powder, its preparation method and application
  • A kind of Bacillus velei zsy-1 wettable powder, its preparation method and application
  • A kind of Bacillus velei zsy-1 wettable powder, its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0018] A wettable powder of Bacillus Velez ZSY-1, which is made of the following raw materials in percentage by weight: 70% of fermentation broth of Bacillus Velez ZSY-1, 6% of sodium carboxymethylcellulose, and BX 4 powder %, the stabilizer sodium carboxymethylcellulose 2%, and the balance is the carrier wheat bran; wherein, the Bacillus Velez ZSY-1 is preserved in the General Microorganism Center of the China Committee for the Collection of Microorganisms, and the preservation number is: CGMCC No.16230, the deposit date is August 8, 2018.

[0019] In addition, the present invention also provides a preparation method of Bacillus velei ZSY-1 wettable powder, comprising the following steps:

[0020] 1) Take 20 μL of Bacillus Velez ZSY-1 stored at -80°C and spread it on a fresh NA plate, and activate it at a constant temperature of 26°C for 24 hours to obtain activated Bacillus Velez ZSY-1;

[0021] 2) Use an inoculation loop to take 1 loop of activated Bacillus velei ZSY-1, in...

Embodiment 2

[0025] A wettable powder of Bacillus Velez ZSY-1, made of the following raw materials in percentage by weight: 70% of fermentation broth of Bacillus Velez ZSY-1, 8% of sodium lignosulfonate, 2% of penetrant T, The stabilizer sodium carboxymethylcellulose is 2%, and the balance is carrier wheat bran; wherein, the Bacillus Velez ZSY-1 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No. 16230, the deposit date is August 8, 2018.

[0026] In addition, the present invention also provides a preparation method of Bacillus velei ZSY-1 wettable powder, comprising the following steps:

[0027] 1) Take 20 μL of Bacillus Velez ZSY-1 stored at -80°C and spread it on a fresh NA plate, and activate it at a constant temperature of 26°C for 24 hours to obtain activated Bacillus Velez ZSY-1;

[0028] 2) Use an inoculation loop to take 1 loop of activated Bacillus velei ZSY-1, inoculate it i...

Embodiment 3

[0032] A wettable powder of Bacillus Velez ZSY-1, made of the following raw materials in weight percentage: 70% of fermentation broth of Bacillus Velez ZSY-1, 3% of diffusing agent MF, Silok-71102%, stabilizer carboxylate Methylcellulose sodium 2%, the balance is carrier wheat bran; wherein, the Bacillus Velez ZSY-1 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No.16230, preserved The date is August 8, 2018.

[0033] In addition, the present invention also provides a preparation method of Bacillus velei ZSY-1 wettable powder, comprising the following steps:

[0034] 1) Take 20 μL of Bacillus Velez ZSY-1 stored at -80°C and spread it on a fresh NA plate, and activate it at a constant temperature of 26°C for 24 hours to obtain activated Bacillus Velez ZSY-1;

[0035] 2) Use an inoculation loop to take 1 loop of activated Bacillus velei ZSY-1, inoculate it into fresh BPY as...

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Abstract

The invention discloses a wettable powder of Bacillus Velez ZSY-1 and its preparation method and application, which is prepared from the following raw materials in percentage by weight: 70% of fermentation liquid of Bacillus Velez ZSY-1, dispersant 3-10 %, wetting agent 2-4%, stabilizer 2%, and the balance is carrier wheat bran; the fermentation broth of Bacillus Velesi ZSY-1, dispersant, wetting agent, stabilizer, and the balance are carrier wheat bran Mix evenly, dry in a freeze dryer, pulverize in a pulverizer to make the fineness less than or equal to 44 μm, and seal and store to obtain Bacillus Velez ZSY‑1 wettable powder. Compared with the prior art, the Bacillus lacei ZSY‑1 wettable powder of the present invention can be used in the early stage or early stage of tomato gray mold disease, and can play a good role in prevention and treatment.

Description

technical field [0001] The invention relates to the field of tomato gray mold and early blight disease prevention and control, in particular to a wettable powder of Bacillus velei ZSY-1 and its preparation method and application. Background technique [0002] Tomato gray mold and early blight are two worldwide diseases in the tomato cultivation process, which occur every year in various cultivation areas, not only have an important impact on tomato yield and quality, but also have an important impact on tomato postharvest storage and preservation Therefore, many researchers have conducted a lot of research on its preharvest and postharvest diseases in recent years. The research directions mainly involve chemical control, physical control, breeding of resistant varieties, biological control, drug resistance and occurrence regularity, etc. Among them, chemical control and drug resistance are more studied, followed by biological control, and some progress has been made. Althou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
CPCA01N25/14A01N63/10
Inventor 刘慧芹高振峰刘慧平刘真真韩巨才王俊学沈高峰
Owner TIANJIN AGRICULTURE COLLEGE