A kind of Bacillus velei zsy-1 wettable powder, its preparation method and application
A technology of Bacillus velesi and ZSY-1, which is applied in the field of tomato gray mold and early blight disease control, can solve problems such as weak research, gaps in the number of active strains or plant numbers in field application research, and difficulties in chemical control.
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Embodiment 1
[0018] A wettable powder of Bacillus Velez ZSY-1, which is made of the following raw materials in percentage by weight: 70% of fermentation broth of Bacillus Velez ZSY-1, 6% of sodium carboxymethylcellulose, and BX 4 powder %, the stabilizer sodium carboxymethylcellulose 2%, and the balance is the carrier wheat bran; wherein, the Bacillus Velez ZSY-1 is preserved in the General Microorganism Center of the China Committee for the Collection of Microorganisms, and the preservation number is: CGMCC No.16230, the deposit date is August 8, 2018.
[0019] In addition, the present invention also provides a preparation method of Bacillus velei ZSY-1 wettable powder, comprising the following steps:
[0020] 1) Take 20 μL of Bacillus Velez ZSY-1 stored at -80°C and spread it on a fresh NA plate, and activate it at a constant temperature of 26°C for 24 hours to obtain activated Bacillus Velez ZSY-1;
[0021] 2) Use an inoculation loop to take 1 loop of activated Bacillus velei ZSY-1, in...
Embodiment 2
[0025] A wettable powder of Bacillus Velez ZSY-1, made of the following raw materials in percentage by weight: 70% of fermentation broth of Bacillus Velez ZSY-1, 8% of sodium lignosulfonate, 2% of penetrant T, The stabilizer sodium carboxymethylcellulose is 2%, and the balance is carrier wheat bran; wherein, the Bacillus Velez ZSY-1 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No. 16230, the deposit date is August 8, 2018.
[0026] In addition, the present invention also provides a preparation method of Bacillus velei ZSY-1 wettable powder, comprising the following steps:
[0027] 1) Take 20 μL of Bacillus Velez ZSY-1 stored at -80°C and spread it on a fresh NA plate, and activate it at a constant temperature of 26°C for 24 hours to obtain activated Bacillus Velez ZSY-1;
[0028] 2) Use an inoculation loop to take 1 loop of activated Bacillus velei ZSY-1, inoculate it i...
Embodiment 3
[0032] A wettable powder of Bacillus Velez ZSY-1, made of the following raw materials in weight percentage: 70% of fermentation broth of Bacillus Velez ZSY-1, 3% of diffusing agent MF, Silok-71102%, stabilizer carboxylate Methylcellulose sodium 2%, the balance is carrier wheat bran; wherein, the Bacillus Velez ZSY-1 is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No.16230, preserved The date is August 8, 2018.
[0033] In addition, the present invention also provides a preparation method of Bacillus velei ZSY-1 wettable powder, comprising the following steps:
[0034] 1) Take 20 μL of Bacillus Velez ZSY-1 stored at -80°C and spread it on a fresh NA plate, and activate it at a constant temperature of 26°C for 24 hours to obtain activated Bacillus Velez ZSY-1;
[0035] 2) Use an inoculation loop to take 1 loop of activated Bacillus velei ZSY-1, inoculate it into fresh BPY as...
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