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A method for effectively degrading drug resistance genes in citrus processing wastewater

A technology for drug-resistant genes and processing wastewater, which is applied in the food industry wastewater treatment, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of increasing drug-resistant genes and difficult to eliminate high-resistant drug-resistant bacteria, etc. Achieve the effect of promoting industrial application, reducing the possibility of drug resistance gene transmission, and optimizing wastewater treatment process

Active Publication Date: 2020-07-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional sewage treatment technologies can effectively kill non-resistant bacteria, it is difficult to eliminate highly resistant drug-resistant bacteria, and some studies have even shown that some technologies may increase the level of drug-resistant genes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A low-temperature plasma technology treatment

[0024] (1) drug resistance gene suspension

[0025] Escherichia coli strain DH5α was inoculated in common broth medium and cultured on a shaker at 37°C until logarithmic growth phase. The ampicillin-resistant and tetracycline-resistant gene plasmids in Escherichia coli were extracted using a plasmid mini-extraction kit, and the ampicillin-resistant and tetracycline-resistant genes were dissolved in the citrus processing wastewater at a concentration of 8-9 log copy number / mL.

[0026] (2) Low temperature plasma treatment

[0027] Experimental group: use double dielectric barrier discharge low-temperature plasma CTP-2000K device to treat citrus processing wastewater containing drug-resistant genes, specific steps: put 3mL of citrus processing wastewater containing drug-resistant genes into a quartz glass with a diameter of 50mm for cultivation In the dish, the air gap distance between the upper medium and the liquid surfa...

Embodiment 2

[0039] A low-temperature plasma technology treatment

[0040] (1) The drug-resistant gene suspension is the same as the method of step (1) in Example 1.

[0041] (2) Low temperature plasma treatment

[0042] The method of one step (2) in embodiment 1 is basically the same, only the processing time is changed to 6min.

[0043] Two detection

[0044] Same as the method of two in embodiment 1.

[0045]The results are as follows: the gene level of ampicillin resistance in the control group was 8.48log copy number / mL, and the gene level of tetracycline resistance was 8.68log copy number / mL. The gene level of ampicillin resistance in the experimental group was 5.22 log copy number / mL, and the gene level of tetracycline resistance was 5.55 log copy number / mL. Therefore, the ampicillin-resistant gene level was degraded to 3.26 log copy number / mL, and the degradation rate was 99.9%, and the tetracycline-resistant gene level was degraded to 3.14 log copy number / mL, and the degradati...

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Abstract

The invention discloses a method for effectively degrading drug resistance genes in citrus processing wastewater. The method comprises the following steps: treating citrus processing wastewater containing drug resistance genes by adopting low-temperature plasma, and then detecting the level of the drug resistance genes in the wastewater, wherein treatment conditions for the low-temperature plasmaare as follows: a treatment frequency is 10-14 kHz, a treatment power is 40-60 W, a treatment time is 4-6 min, and a treatment temperature is 20-30 DEG C. The method disclosed by the invention adoptsa low-temperature plasma technology for effectively reducing the content of the drug resistance genes in the citrus processing wastewater, so as to further control the propagation of the drug resistance genes in an ecological environment.

Description

technical field [0001] The invention relates to the field of wastewater treatment, in particular to a method for effectively degrading drug resistance genes in citrus processing wastewater. Background technique [0002] Antimicrobial resistance has posed a global threat to public health, food safety, and economic development. The misuse of antibiotics has accelerated the wider spread of bacterial resistance genes in the environment. Drug resistance genes are mobile genetic elements that can be transferred from one bacterium to another by the principle of horizontal gene transfer. Once large numbers of resistance genes are released into the environment, there is a good chance that other bacteria will acquire them, leading to resistance to antibiotics. Drug resistance genes have been considered as one of the common hazards in ecosystems (such as urban sewage, agricultural industrial wastewater, drinking water, etc.). Although traditional sewage treatment technologies can ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F1/46C12Q1/6851C02F103/32
CPCC02F1/4608C02F2103/32C12Q1/6851C12Q2531/113C12Q2563/107
Inventor 丁甜廖新浴刘东红叶兴乾陈健初胡亚芹陈士国
Owner ZHEJIANG UNIV
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