Kit for helping to detect cancer antigen of sub-health people

A technology of auxiliary detection and kit, which is applied in the field of kits for auxiliary detection of carcinoembryonic antigen in sub-healthy population, which can solve the problems of complex operation, quantitative analysis of carcinoembryonic antigen, and high cost

Inactive Publication Date: 2019-01-18
北京莱科百奥生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nowadays, the common detection methods for carcinoembryonic antigen include radioimmunoassay and indirect immunofluorescence. Radioimmunofluorescence is expensive and requires sophisticated and complex equipment to prevent radioactive contamination. Indirect immunofluorescence is complex and...

Method used

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  • Kit for helping to detect cancer antigen of sub-health people
  • Kit for helping to detect cancer antigen of sub-health people
  • Kit for helping to detect cancer antigen of sub-health people

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A kit for auxiliary detection of cancer embryonic antigen in sub-healthy population, which is composed of the following components: carcinoembryonic antigen calibrator, cancer embryonic antigen quality control product, reagent No. 1, reagent No. 2, magnetic separation reagent, chemical substrate and washing solution .

[0036] The preparation method of the kit for auxiliary detection of sub-healthy population cancer blank antigen is carried out according to the following steps:

[0037] (1) prepare carcinoembryonic antigen calibrator: Potassium dihydrogen phosphate powder 0.3g, disodium hydrogen phosphate powder 1.5g, sodium chloride powder 8g, potassium chloride powder 0.3g, concentration 98% Proclin300 solution 0.15g, remove Add 800g of ionized water into the container, stir until it is completely dissolved, add 0.5g of hydrochloric acid with a concentration of 36% to 38%, adjust the pH value to 7.3, add 32g of bovine serum albumin into the container, stir until it is...

Embodiment 2

[0045] A kit for auxiliary detection of cancer blank antigen in sub-healthy population, which consists of the following components: carcinoembryonic antigen calibrator, cancer blank antigen quality control product, reagent No. 1, reagent No. 2, magnetic separation reagent, chemical substrate and washing solution .

[0046] The preparation method of the kit for auxiliary detection of sub-healthy population cancer blank antigen is carried out according to the following steps:

[0047](1) Prepare carcinoembryonic antigen calibrator: Potassium dihydrogen phosphate powder 0.2g, disodium hydrogen phosphate powder 1g, sodium chloride powder 7g, potassium chloride powder 0.2g, concentration 98% Proclin300 solution 0.1g, deionized Add 700g of water into the container, stir until it is completely dissolved, add 0.1g of hydrochloric acid with a concentration of 36% to 38%, adjust the pH value to 7.4, add 30g of bovine serum albumin into the container, stir until it is completely dissolve...

Embodiment 3

[0055] A kit for auxiliary detection of cancer embryonic antigen in sub-healthy population, which is composed of the following components: carcinoembryonic antigen calibrator, cancer embryonic antigen quality control product, reagent No. 1, reagent No. 2, magnetic separation reagent, chemical substrate and washing solution .

[0056] The preparation method of the kit for auxiliary detection of sub-healthy population cancer blank antigen is carried out according to the following steps:

[0057] (1) Prepare carcinoembryonic antigen calibrator: Potassium dihydrogen phosphate powder 0.4g, disodium hydrogen phosphate powder 2g, sodium chloride powder 9g, potassium chloride powder 0.4g, concentration 98% Proclin300 solution 0.2g, deionized Add 900g of water into the container, stir until it is completely dissolved, add 1g of hydrochloric acid with a concentration of 36% to 38%, adjust the pH value to 7.2, add 34g of bovine serum albumin into the container, stir until it is completel...

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Abstract

The invention discloses a kit for helping to detect a cancer antigen of sub-health people, and belongs to the technical field of biology. The kit comprises a cancer antigen calibration product, a cancer antigen quality control product, a first Tris buffer liquid, a second buffer liquid, a third Tris buffer liquid, a chemical substrate and a washing liquid, wherein the first Tris buffer liquid includes a biotin labeled cancer antigen monoclonal antibody and bovine serum albumin, the second buffer liquid includes an alkaline phosphatase labeled cancer antigen monoclonal antibody and the bovine serum albumin, and the third Tris buffer liquid includes streptavidin labeled magnetic particle and the bovine serum albumin. The kit is high in sensitivity, low in cost and rapid and reliable in reaction process and is simple to operate, no radiation pollution is generated, the sensitivity is improved, the linear range is expanded, quantitative measurement is achieved, and an appropriate theoretical judgment is provided for illness of conventional disease of the sub-health people and screening of broad-spectrum tumor.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a kit for assisting detection of cancer blank antigens in sub-healthy populations. Background technique [0002] Sub-health refers to a state in which the human body is between health and disease. Those who are in a sub-health state cannot meet the health standards, and manifest as symptoms of reduced vitality, function and adaptability within a certain period of time, which do not meet the clinical or sub-clinical diagnostic criteria for diseases related to modern medicine, but the concept of sub-health has a certain significance. It has very important medical value and clinical significance, because it can advance the critical period of disease prevention and treatment. In recent years, due to environmental pollution, fierce competition, the pace of people's life and work has been accelerating, the pressure from society and all aspects has been increas...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/538G01N33/574G01N33/58G01N33/531G01N33/535
CPCG01N33/54326G01N33/531G01N33/535G01N33/538G01N33/57492G01N33/581G01N2333/47
Inventor 赵彦涛
Owner 北京莱科百奥生物技术有限公司
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