A composition for improving the oil yield and purity of Rhodotorula viscosus and its preparation method and use method

A technology for Rhododendron viscosus and composition, which is applied in the field of compositions and preparations for improving the yield and purity of oil of Rhododendron viscosus, can solve the problems of oil loss, high product impurity content, complicated operation and the like, and achieves improved yield of finished products , reduce the impurity content, improve the effect of high quality

Active Publication Date: 2022-05-06
海南煊烨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The n-hexane used in this method still has a certain low toxicity, and the product contains more impurities, and the loss of oil after repeated solid-liquid separation, in addition, the operation is more cumbersome, and there are certain limitations in the popularization and application scheme.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A composition for improving the oil yield of Rhodotorula viscosus, the composition includes the following ingredients in parts by weight: 2 parts of verbena, 2 parts of houttuynia, 3 parts of baby's breath and 2 parts of chitosan.

[0022] The preparation method of described composition comprises the following steps:

[0023] Take Verbena, Houttuynia cordata and Gypsophila gypsophila, dry, pulverize, and pass through a 200-mesh sieve to obtain powder. Add 80% ethanol aqueous solution to the powder, and the amount of ethanol water per gram of powder is 20mL, 45 Extract at ℃ for 1 hour, filter to obtain a filter cake, add warm water at 30℃ to extract for 45 minutes according to the ratio of material to liquid g / mL of 1:2, filter, take the filtrate, concentrate under reduced pressure to obtain an extract with a water content of less than 8%, add shell polysaccharide, mix well and serve.

Embodiment 2

[0025] A composition for increasing the oil yield of Rhodotorula viscosus, the composition includes the following ingredients in parts by weight: 3 parts of verbena, 3 parts of houttuynia, 5 parts of baby's breath and 2 parts of chitosan.

[0026] The preparation method of the composition is the same as in Example 1.

Embodiment 3

[0028] A composition for increasing the oil yield of Rhodotorula viscosus, the composition comprising the following components: 2 parts of verbena, 2 parts of houttuynia, 5 parts of baby's breath and 1 part of chitosan.

[0029] The preparation method of the composition is the same as in Example 1.

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PUM

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Abstract

The invention provides a composition for improving the oil yield and purity of Rhodotorula viscosus and its preparation method and application method. The composition of the present invention includes 2 to 3 parts of Verbena, 2 to 3 parts of Houttuynia cordata, 3 to 5 parts of Gypsophila gypsophila and 1 to 2 parts of chitosan. Adsorption, through adsorption, significantly reduces the impurity content in the microbial oil and improves the quality of the oil; the composition of the invention can promote the cell wall breaking of Rhodotorula viscosus, completely release the microbial oil from the cells, and increase the yield of finished products. By adopting the method of the invention, the highest yield of rhodotorula viscosus oil reaches 99.3%, the impurity content in the obtained microbial oil can be as low as 0, the unsaponifiable matter content is as low as 0.1%, and the oil quality is good.

Description

technical field [0001] The invention relates to the technical field of microbial oil extraction, in particular to a composition for improving the oil yield and purity of Rhodotorula viscosus and its preparation method and use method. Background technique [0002] Rhodotorula glutinis (Fres.) Harrison is an important industrial strain with a short growth cycle and rich oil content, which can generally reach 40% to 70% of the dry cell weight, and the oil production per unit area can be high It is an oil-producing microorganism with good application prospects. Rhodotorula viscosus has a large body and many metabolites. It is rich in protein and a lot of pigments. It is difficult to extract high-quality and high-yield microbial oil from Rhodotorula viscosus. [0003] CN104531342A discloses a method for extracting microbial oil, comprising the following steps: (1) passing the fermented oil-producing microorganisms through a separation system to obtain wet bacteria with a water c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/02
CPCC11B1/025
Inventor 张国栋
Owner 海南煊烨生物科技有限公司
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