A bioreactor that simulates intraocular pressure

A technology of bioreactor and intraocular pressure, which is applied in specific-purpose bioreactor/fermenter, combination of bioreactor/fermenter, biochemical instrument, etc. Problems such as limited thickness and disordered arrangement of matrix fibers can achieve the effect of ensuring micro-circulation flow, improving nutrient supply and metabolite transportation, and promoting the formation of extracellular matrix

Active Publication Date: 2021-07-09
XIAMEN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The current method of tissue engineering cornea construction is mainly to culture cells on the surface of flat materials to promote the secretion of extracellular matrix on the surface of the material, but the thickness of the secreted extracellular matrix is ​​limited, the arrangement of matrix fibers is chaotic, and the strength is low. Tissue-engineered corneas are less transparent
The reported tissue engineering cornea construction techniques have not considered the influence of intraocular pressure on the tissue engineering cornea construction process

Method used

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  • A bioreactor that simulates intraocular pressure
  • A bioreactor that simulates intraocular pressure
  • A bioreactor that simulates intraocular pressure

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Please check figure 2 , a bioreactor for simulating intraocular pressure in this embodiment, including a culture solution storage bottle 93, a pressure control component, and a tissue culture component;

[0034] The tissue culture assembly includes a main body and 3 tissue culture units 17; using high temperature resistant, high pressure, non-toxic, biocompatible materials (including but not limited to plastic, glass, stainless steel and polycarbonate, etc.), can Recycling, while ensuring a sterile environment.

[0035] The main body includes a loam cake 5 and a base 11, the base 11 is provided with a cavity, the loam cake 5 covers the upper opening of the base 11, and the loam cake 5 and the base 11 surround and form a tissue culture chamber 13; The tissue culture chamber 13 is provided with a culture solution inlet 61 connected to the pressure control assembly and a culture solution outlet 62 connected to the culture solution storage bottle 93 .

[0036] The tissue c...

Embodiment 2

[0041] The difference between Embodiment 2 and Embodiment 1 is that the bioreactor of this embodiment also includes an overflow assembly, which includes an overflow cup 7 and a cup cover 71, and the tissue culture upper button 1 is provided with an overflow hole 2, the overflow hole 2 is connected with the overflow cup 7 through an overflow pipe 8.

[0042] Using this embodiment to simulate the cell culture operation of intraocular pressure is as follows:

[0043] 1. Sterilize the bioreactor under high temperature and high pressure environment;

[0044] 2. In the ultra-clean bench, spread the cell culture carrier material-corneal stromal cell system on the surface of the tissue culture lower buckle 4, and the tooth-shaped buckle of the tissue culture upper buckle 1 and the tooth-shaped buckle of the tissue culture lower buckle 4 are staggered , rotate the tissue culture buckle 1 on the surface of the carrier until the upper and lower toothed buckles are engaged together;

[...

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Abstract

The invention discloses a bioreactor for simulating intraocular pressure, which is used for constructing tissue engineering cornea. The reactor provides corneal stromal cells and corneal endothelial cells with mechanical pressure similar to intraocular pressure through continuous circulation of culture fluid, better maintains the characteristics of corneal stromal cells and endothelial cells, promotes the production of extracellular matrix, and improves tissue engineering The transparency and mechanical toughness of the cornea make the tissue-engineered cornea more consistent with the biological characteristics of the cornea in vivo.

Description

technical field [0001] The invention relates to a bioreactor for simulating intraocular pressure. Background technique [0002] The structure and composition of human organs and tissues are mainly determined by genes, and are constantly modified and reshaped by environmental factors during development. Extensive research has now found that the structure and composition of connective tissue changes in response to the changing mechanical stresses it is exposed to in vivo. Studies in animals and in vitro models have found that mechanical stress can induce changes in the structure of organs and tissues including bones, muscles, fascia, blood vessels, and cornea, and mechanical stress is also an important factor for the normal development of connective tissue in vivo. [0003] Vascular tissue is one of the organs in the body that changes in response to changes in mechanical stress. In the process of angiogenesis and development, changes in vessel diameter are related to blood f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/36C12M1/34
CPCC12M21/08C12M23/38C12M23/48C12M29/14C12M41/40C12M41/48
Inventor 李炜车欣刘祖国赵艳媛陈延平
Owner XIAMEN UNIV
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