DNA probe and method for detecting dna single base mutation

A technology of DNA probe and single-base mutation, which is applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of high professional requirements for operators, complex detection method design, and cumbersome professional operation, etc., to achieve Low professional requirements, easy operation, good effect

Active Publication Date: 2022-05-06
XIANGTAN UNIV
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Problems solved by technology

[0004] Aiming at the above technical problems, the present invention provides DNA probes and methods for detecting DNA single base mutations, which solve the problems in the prior art such as complex design of detection methods, expensive instruments, cumbersome professional operations, and high professional requirements for operators.

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  • DNA probe and method for detecting dna single base mutation
  • DNA probe and method for detecting dna single base mutation
  • DNA probe and method for detecting dna single base mutation

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Embodiment Construction

[0035] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0036] The naming of the DNA sequence of the mutation target in the embodiment represents its different base types of mismatch sites, and does not aim at specific functions, emphasizing the versatility of the method; the mixed solution of DNA of different mutation targets is obtained by artificial mixing, The discriminative ability of the method to identify different proportions of mismatches. Complex samples are obtained by adding high concentrations of protist DNA to the buffer solution to simulate the complex sample environment in actual analysis and testing, emphasizing the anti-interference ability of the method. The chemically synthesized mutation target used in the present invention is provided by Shanghai Sangong Co., Ltd. The schematic diagram of the reaction process of the detection method of the present in...

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Abstract

The invention belongs to the technical field of molecular detection, and provides a DNA probe and a method for detecting DNA single-base mutations. The sequence of the DNA probe is shown in SEQ ID NO: 1-9. After diluting the probe solution, it is boiled, annealed, and cooled to room temperature. , add the mutation target substance for constant temperature reaction, then add the mixture of 7Ff-3A and E-Q, measure the fluorescence spectrum intensity of the mixture, perform factor analysis, and finally obtain the differentiation result. The invention has the advantages of accurate and convenient detection of environmental samples, good specificity and high sensitivity of the detection method.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a DNA probe and a method for detecting DNA single base mutations. Background technique [0002] DNA single-base mutations, including single-base substitutions, insertions, and deletions, are important biomarkers of human disease and drug resistance. For example, the molecular basis of thalassemia is a single base mutation in the gene sequence on the short arm of chromosome 11, resulting in the failure of normal synthesis of related proteins and inducing thalassemia. [0003] Existing single-base mismatch detection methods include polymerase chain amplification, next-generation sequencing, microarray, and in situ hybridization. These methods have been commercially applied, but they require expensive instruments, complicated operations and highly professional operators, making it difficult to be applied in ordinary laboratories and enterprises. At the same...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12Q1/6827
CPCC12Q1/6827C12Q1/6883C12Q2600/156C12Q2563/107
Inventor 杨斌李叶杜军
Owner XIANGTAN UNIV
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