DNA probe and method for detecting dna single base mutation

Abstract

The invention belongs to the technical field of molecular detection, and provides a DNA probe and a method for detecting DNA single-base mutations. The sequence of the DNA probe is shown in SEQ ID NO: 1-9. After diluting the probe solution, it is boiled, annealed, and cooled to room temperature. , add the mutation target substance for constant temperature reaction, then add the mixture of 7Ff-3A and E-Q, measure the fluorescence spectrum intensity of the mixture, perform factor analysis, and finally obtain the differentiation result. The invention has the advantages of accurate and convenient detection of environmental samples, good specificity and high sensitivity of the detection method.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and in particular relates to a DNA probe and a method for detecting DNA single base mutations. Background technique [0002] DNA single-base mutations, including single-base substitutions, insertions, and deletions, are important biomarkers of human disease and drug resistance. For example, the molecular basis of thalassemia is a single base mutation in the gene sequence on the short arm of chromosome 11, resulting in the failure of normal synthesis of related proteins and inducing thalassemia. [0003] Existing single-base mismatch detection methods include polymerase chain amplification, next-generation sequencing, microarray, and in situ hybridization. These methods have been commercially applied, but they require expensive instruments, complicated operations and highly professional operators, making it difficult to be applied in ordinary laboratories and enterprises. At the same...

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Problems solved by technology

[0004] Aiming at the above technical problems, the present invention provides DNA probes and methods for detecting DNA single base mutations, which solve the problems in the prior art such as complex design of detection methods, expensive instruments, cumbersome professional operations, and high professional requirements for operators.

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