Application of active ingredient perillaldehyde in prevention and treatment of oropharyngeal candidiasis

A technology of candidiasis and active ingredients, applied in the new use field of perillaldehyde, can solve problems such as unseen oropharyngeal candidiasis

Active Publication Date: 2019-02-15
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are only a few reports on the antifungal effect of PAE, and there are few reports on the anti-candida albicans of peri

Method used

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  • Application of active ingredient perillaldehyde in prevention and treatment of oropharyngeal candidiasis
  • Application of active ingredient perillaldehyde in prevention and treatment of oropharyngeal candidiasis
  • Application of active ingredient perillaldehyde in prevention and treatment of oropharyngeal candidiasis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Determination of the effect of perillaldehyde on the acidification of the external fluid of Candida albicans

[0027] Dilute the Candida albicans cell suspension to 10 with sterile PBS 7CFU / ml, add to SDB at 200rpm, shake at 30°C for 48h. Transfer the bacterial suspension to a 50ml centrifuge tube, centrifuge at 5000rpm for 10min, discard the supernatant. Add 20ml sterile water to resuspend and wash twice. And Candida albicans was resuspended in 50mM potassium chloride solution to make the final volume 40ml. The bacterial suspension was placed in a 4°C refrigerator for overnight starvation treatment. The next day, different concentrations of perillaldehyde were added so that the final concentrations were 0.4 μl / ml, 0.6 μl / ml, and 0.8 μl / ml. The control group was added with the same volume of Tween 80, and the final volume was adjusted to 50 mM potassium chloride solution. Adjust to 45ml and incubate at room temperature for 10min. Centrifuge again at 5000...

Embodiment 2

[0030] Example 2: The protective effect of perillaldehyde on oropharyngeal candidiasis

[0031] Perillaldehyde has a good protective effect on oropharyngeal candidiasis caused by Candida albicans.

[0032] 1. Experimental grouping

[0033] After the mice were caged, they were randomly divided into 6 groups. The Control group was the growth control group not infected with Candida albicans; the Ca group was the group infected with Candida albicans without administration; the 2mg / kg PAE group was the group infected with Candida albicans and The treatment group was administered with 2mg / kg PAE daily; the 3mg / kg PAE group was infected with Candida albicans and administered daily with 3mg / kg PAE; the 4mg / kg PAE group was infected with Candida albicans and administered daily with Body weight 4mg / kg PAE treatment group; 20mg / kg FCZ group is the control group infected with Candida albicans and daily body weight 20mg / kg FCZ.

[0034] 2. Model construction

[0035] In this experiment,...

Embodiment 3

[0051] Example 3: Inhibition of perillaldehyde on NLRP3 inflammasome

[0052] The effect of perillaldehyde on mouse NLRP3 inflammasome detected by Western Blot

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Abstract

The invention discloses application of a natural active ingredient perillaldehyde in the prevention and treatment of oropharyngeal candidiasis, and belongs to the technical field of medicine. The invention also discloses that the monoterpenoid compound perillaldehyde can be targeted to three major virulence factors of candida albicans: adhesion, hyphal phase transformation and secretion of hydrolase to inhibit the damage of candida albicans to the host and to achieve antibacterial and anti-inflammatory effects by regulating the NLRP3 inflammasome in vivo. By using real-time fluorescence quantification, immunofluorescence, ultraviolet spectrum scanning and other techniques, the perillaldehyde is found to have strong resistance to the candida albicans at a low dose of 0.4 mul/ml.

Description

technical field [0001] The invention relates to a new application of perillaldehyde, which belongs to the technical field of medicine. Background technique [0002] Nowadays, with the continuous improvement of human living standards and medical standards, ideological emancipation, and the serious phenomenon of population aging in our country; the incidence of immunodeficiency diseases such as diabetes, AIDS, cancer and tumors continues to rise; denture technology, organ transplantation technology The extensive use of immunosuppressants and antibiotics, chemotherapy technology, intravenous hypertrophic nutrition technology, makes the clinical fungal infection rate is on the rise, and poses a huge threat to people's life and health, and has become the main factor of death in patients with such infections ( 1). Candida albicans is the main conditional pathogenic fungal pathogen that threatens the lives of patients clinically. It can cause diseases, but when the micro-ecologic...

Claims

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Application Information

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IPC IPC(8): A61K31/11A61P31/10A61P29/00A61P1/02
CPCA61K31/11A61P1/02A61P29/00A61P31/10
Inventor 田俊陈磊王瑱潘沈元彭学贺晓娜李永新刘曼杨坤龙
Owner XUZHOU NORMAL UNIVERSITY
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