Method for detecting heavy metal content in tea

A detection method and technology for heavy metals, which are applied in measurement devices, preparation and sampling of samples for testing, etc., can solve problems such as unfriendly environment, inability to directly apply the liquid to be tested, and potential safety hazards in manual operation, and achieve the effect of safe operation.

Active Publication Date: 2019-02-15
利川市纪元茶业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the pretreatment is to convert the sample into a liquid solution for measurement, and the test solution obtained by the above pretreatment method is strongly acidic, which is not only unfriendly to the environment, but also has potential safety hazards in manual operation. In addition, the strong acid test solution cannot For all detection methods, such as enzyme-linked immunoassay cannot be carried out in a strong acid environment, so the test solution obtained by traditional pretreatment methods cannot be directly applied to enzyme-linked immunoassay. Therefore, the applicant based on the current detection process The pre-treatment method has the defects of strong acidity and cannot be used for ELISA, and the pre-treatment method is improved to overcome the above-mentioned defects

Method used

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  • Method for detecting heavy metal content in tea

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Effect test

Embodiment 1

[0020] The present embodiment a kind of detection method of tea heavy metal content, comprises the following steps:

[0021] (1) Sample pretreatment: pulverize the tea leaves to be tested to 60 mesh, add 8 times the amount of water, 300W ultrasonic treatment for 30 minutes to obtain a treatment solution, add 15% of the treatment solution weight heavy metal flocculant to the treatment solution and mix and stir Evenly, let it stand until the flocculent precipitate precipitates, centrifuge, collect the precipitate, wash with water until there is no powder on the surface of the precipitate;

[0022] (2) Extraction of heavy metal test solution: add 0.5% α-glucosidase of the precipitate weight to the above precipitate, enzymolyze the precipitate in a water bath at 40°C until the precipitate is completely dissolved, and inactivate the enzyme activity at 120°C under high temperature. Measuring liquid;

[0023] (3) Detection of heavy metal content: the content of lead, chromium, cadmi...

Embodiment 2

[0026] The present embodiment a kind of detection method of tea heavy metal content, comprises the following steps:

[0027] (1) Sample pretreatment: crush the tea leaves to be tested to 80 mesh, add 10 times the amount of water, 600W ultrasonic treatment for 50min, to obtain a treatment solution, add 30% of the treatment solution weight heavy metal flocculant to the treatment solution and mix and stir Evenly, let it stand until the flocculent precipitate precipitates, centrifuge, collect the precipitate, wash with water until there is no powder on the surface of the precipitate;

[0028] (2) Extraction of heavy metal test solution: add α-glucosidase of 1.5% of the weight of the precipitate to the above precipitate, enzymatically hydrolyze the precipitate in a water bath at 55°C until the precipitate is completely dissolved, and inactivate the enzyme activity at a high temperature at 150°C. Measuring liquid;

[0029] (3) Detection of heavy metal content: the content of lead, ...

Embodiment 3

[0032] The present embodiment a kind of detection method of tea heavy metal content, comprises the following steps:

[0033] (1) Sample pretreatment: pulverize the tea leaves to be tested to 70 mesh, add 9 times the amount of water, 400W ultrasonic treatment for 40min, to obtain a treatment solution, add a heavy metal flocculant with 26% of the treatment solution weight to the treatment solution, mix and stir Evenly, let it stand until the flocculent precipitate precipitates, centrifuge, collect the precipitate, wash with water until there is no powder on the surface of the precipitate;

[0034] (2) Extraction of heavy metal test solution: add α-glucosidase of 1% of the weight of the precipitate to the above precipitate, enzymatically hydrolyze the precipitate in a water bath at 50°C until the precipitate is completely dissolved, and inactivate the enzyme activity at a high temperature at 130°C. Measuring liquid;

[0035] (3) Detection of heavy metal content: the content of l...

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Abstract

The invention discloses a method for detecting heavy metal content in tea, and relates to the technical field of detection of heavy metal in food. The method comprises the following steps of: crushingthe tea to be detected, adding into water, and performing ultrasonic treatment; adding a heavy metal flocculant to precipitate precipitation, adding glycosidase to the precipitation, and performing enzymatic hydrolysis of the precipitation in a water bath until precipitation is completely dissolved, so as to obtain a to-be-detected liquid; and detecting the content of lead, chromium, cadmium, andcopper of the to-be-detected liquid by enzyme linked immunosorbant assay. The method for detecting the heavy metal content in the tea obtains the to-be-detected liquid containing no acid by optimizedthe pretreatment method, is safety and environmental friendliness, and can be used for the enzyme linked immunosorbant assay.

Description

【Technical field】 [0001] The invention relates to the technical field of food heavy metal detection, in particular to a method for detecting heavy metal content in tea. 【Background technique】 [0002] Tea is one of the three major beverages in the world. In recent years, the rapid development of industrialization and urbanization has exposed tea production in many places to polluted environments. The content of heavy metals such as lead, chromium, cadmium, and copper in tea has an upward trend. Long-term Drinking tea with excessive pollutants will cause harm to human health, and heavy metal content testing is required. [0003] The detection of heavy metals in tea mainly includes two steps: pretreatment and detection. The commonly used detection methods at this stage are: atomic absorption spectrophotometry, inductively coupled plasma mass spectrometry, atomic fluorescence spectrometry, enzyme-linked immunoassay, etc., and the commonly used pretreatment methods are wet dige...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/40G01N33/535
CPCG01N1/28G01N1/286G01N1/4055G01N33/535
Inventor 谢宗宜
Owner 利川市纪元茶业有限责任公司
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