107results about How to "Facilitate follow-up testing" patented technology

The invention discloses a kit for methylation of colorectal cancer related gene multi-sites and application of the kit. A primer combination and a probe can specifically detect the methylation degrees of a plurality of colorectal cancer related gene sites. The invention further provides application and a method of the primer combination and the probe for detecting the methylation in colorectal cancer and precancerous polyps screening. The method comprises the steps that a cell lysis solution is added to cell sediment and mixed uniformly, then PVPP is added for crude extraction, a cell sediment sample after primary treatment contains a little protein and RNA, protease K and RNA enzyme are added for completely removing the protein and the RNA, cell lysate is added to a centrifugal column with an adsorption film, after impurities are washed by a DNA rising solution, a DNA eluent is added to elute the DNA in the absorption film, and finally a DNA solution is collected through centrifugation. Efficient pipe collection detection is performed aiming at the plurality of gene sites, the flexibility and the specificity are improved, and the tumor early screening requirement can be improved.

The invention relates to an auto part defect recognition method based on similarity match. The method has functions of detecting a leakage welding point, a leakage small part, a leakage nut and a leakage punching hole on the auto parts, and the detection objects are a welding point, a small part, a nut and a punching hole. The method comprises realization steps: firstly, an auto part image is acquired, and whether a template library needs to be built is judged; then, a detection area is captured on the acquired image, and image preprocessing is carried out on the area; then, template images ina detection object template library are traversed, the template images and the image after pre-processing are subjected to scanning and similarity contrast, a contrast result is saved to a cue map, and all information of the detection objects is included in the cue map; and finally, contour detection processing is carried out on the cue map, a contour perimeter, a roundness and an area are used to select a detection object, the contour number and position information is acquired, and whether defects of a leakage welding point, a leakage small part, a leakage nut or a leakage punching hole exist on the auto part in the acquired image can be detected.

The invention discloses an exosome nucleic acid detection technology based on magnetism-enriched electrochemical luminescence. The exosome nucleic acid detection technology comprises the steps of sample pretreatment and separation of an exosome standard substance, activation of streptavidin magnetic beads and a biotin antibody, binding of immunomagnetic magnetic beads and an exosome, preparation of exosome nucleic acid sample preparation and target nucleotide sequence detection through electrochemical luminescence. The exosome nucleic acid detection technology has the advantages that exosomesin biological samples such as urine, serum, saliva and a cell culture fluid can be separated and analyzed; antibodies are modified to the surfaces of the magnetic beads through specific binding of biotin and streptavidin, systems are stable, and nonspecific interference is avoided; the systems are controllable, different subtypes of exosomes are separated and purified by changing antibody types, and the technology can be used for nucleic acid expression profile studies and exosome somatotype studies; the final concentration is controllable, required concentration is obtained through re-dilution or re-releasing after magnetic enrichment, and follow-up detection is facilitated; the separation speed is high, the instrument requirement is low, and multiple samples can be treated simultaneously.

The invention discloses a battery silk-screen quality detection method combining block template matching with morphological processing. The method comprises the following steps: establishing a character part template based on a morphological template matching method; extracting a battery area by applying threshold segmentation and morphological corrosion; determining an inclination angle in a mode of solving a minimum circumscribed rectangle of a battery area, searching the minimum circumscribed rectangle as a final result by adopting an equal interval rotation search algorithm, and performing reverse rotation on a deflection angle by utilizing affine transformation to perform battery correction and cutting; identifying the defects of the illustration part by adopting a difference image method; sequentially shielding picture insertion, character segmentation, character extraction and correction, character recombination and to-be-detected character skeleton extraction, identifying character part defects by adopting a difference image method, and carrying out defect detection by adopting suspicious area translation difference detection and suspicious area micro-rotation difference detection. The method is high in accuracy and low in cost, and has a certain degree of promotion effect in the field of domestic battery screen printing quality automatic detection.

The invention provides a diafiltration device, which comprises a sampling component, a top cover and a sample tube body, wherein a through hole is formed in the center of the top cover; the sampling component comprises a connecting cylinder and a sampling part, the connecting cylinder is concentric with the through hole and is connected with the through hole in a rotating manner, the sampling component is concentrically connected with the connecting cylinder and can collect suspension; the sample tube body can be connected with the top cover so that the sample tube body is arranged at the outer side of the sampling component in a sheathed manner. The through hole, the connecting cylinder and the sampling part, which are coaxially arranged, replace a sampling component and an insertion hole which are simultaneously arranged on a top cover with an original structure, the dilution, mixing and sampling operations of the diafiltration device can be realized only by the through hole in the center of the top cover, the problem that the occupation area is large as the sampling component and the insertion hole are simultaneously arranged on the top cover can be solved, the design dimensions of the top cover and the sample tube body can be reduced, and the space resources can be saved. The invention further provides equipment for making a defecate detection solution by matching with the diafiltration device, and a method for making the defecate detection solution.

The invention relates to a Bar detection mechanism and a working method thereof. The mechanism is characterized by comprising a frame, and a Bar carrying mechanism and a Bar moving mechanism which arearranged on the frame, wherein the Bar carrying mechanism includes a base arranged on the frame and a loading disc arranged on the base, the loading disc is driven and displaced by transverse and vertical driving mechanisms arranged on the base and a swinging mechanism, the Bar moving mechanism includes a transverse guide rail arranged above the frame, a transverse sliding base arranged on the transverse guide rail and a lifting sliding base arranged on the transverse sliding base, and the lifting sliding base is provided with a Bar suction head. The mechanism is advantaged in that design isreasonable, convenient and rapid Bar swinging and movement are facilitated, and later detection is facilitated.

The invention provides a liquid drop digital PCR chip, which comprises a chip body. One or more liquid drop generation units are arranged on the chip body and each comprise a liquid injection pond, aliquid drop generation cavity, a liquid drop flat-spreading cavity and an oil injection pond which communicate sequentially, after the liquid drop generation units are fully filled with oil phase reagents, PCR reagents flow out from the liquid drop generation cavities through the liquid injection ponds, then a plurality of liquid drops are formed, and the multiple liquid drops are flat spread in the liquid drop flat-spreading cavities in a single-layer mode. The liquid drop digital PCR chip integrates liquid drop generation and single-layer liquid drop flat-spreading functions, the chip is directly placed in a PCR instrument, thus the liquid drops in the chip can be subjected to PCR reaction, the generated liquid drops do not need to be transferred again, the state of the liquid drops is better maintained, and PCR is facilitated; and the liquid drops are flat spread in thea single-layer mode, the liquid drops are not overlapped, subsequent detection is convenient, the detection efficiency is improved, and the detection accuracy rate is increased.

The invention discloses a sampling apparatus for water quality detection. The sampling apparatus comprises a holding bar, wherein the bottom of the holding bar is fixedly connected with a vertical bar; and the right side of the vertical bar is provided with sampling apparatuses which are equidistantly distributed in the vertical direction. The sampling apparatus is provided with multiple groups ofsampling apparatuses and the vertical bar with a scale line to simultaneously sample water in different depths, so that the sampling accuracy is improved, and the sampling efficiency is increased; the sampling apparatus is provided with a filtering net which is used for screening removing suspension garbage and aquatic plants in the water, so that the purity of the water sample is improved, and the subsequent detection is facilitated; and the sampling apparatus is provided with a pulling bar and a moving bar as well as a sealing cover which is fixedly connected with the moving bar, so that the sampling apparatus is convenient in operation, simple and efficient.

The invention discloses a workpiece surface detection device and method for casting machining. The device comprises a shell, a fixing box is fixedly connected to the top end face of the shell, a fourth motor is arranged at the top of the fixing box, the output end of the fourth motor penetrates through the fixing box, a rotating mechanism is arranged in the fixing box, and two first supporting boxes are symmetrically arranged in the shell; the two first supporting boxes are fixedly connected to the two side walls of the shell correspondingly, two connecting blocks are arranged between the twofirst supporting boxes, a first motor is arranged in one connecting block, a connecting plate is fixedly connected between the two first supporting boxes, and a movable groove is dug in the bottom endface of the connecting plate. The detection device has the beneficial effects that the rotating mechanism can adjust the height of the detector according to the sizes of different workpieces, subsequent detection is facilitated, the moving mechanism can adjust the detector to move left and right, and then the surfaces of the workpieces can be completely detected.

The invention relates to a preparation method of a high-flux bulk material based on a friction stir technology. The preparation method comprises following steps: (1), determining a research target, selecting multiple boards with different components and the same thickness serving as base materials for pretreatment, and clamping and fixing the treated base materials on a processing platform; and (2), setting friction stir processing parameters, completing processing in a certain processing direction, and realizing continuous change of researched components in an obtained bulk material in the processing direction. Compared with the prior art, the components in the prepared material are in gradient change, and samples of the corresponding components can be cut off according to calculation; the preparation process has the advantages of being low in energy consumption, green and environmentally friendly; and experimental equipment does not need to control conditions such as vacuum degree, magnetic field and temperature, the method is simple and easy, and size and performance of the prepared bulk material can be adjusted by the subsequent process.

The invention discloses an overturning platform, which comprises an overturning platform base, a rotating device, a clamping device and an auxiliary positioning device. The rotating device includes a rotating cylinder fixing plate, a rotating shaft support seat, a rotating cylinder and a rotating shaft. The clamping The device includes a clamping frame, an overturning table slide plate, a spring guide shaft, a two-way cylinder and a connecting rod. The clamping frame includes a left connecting plate, a right connecting plate, a support plate and an overturning table fixing plate. Support blocks and several groups of profiling positioning mechanisms, each group of profiling positioning mechanisms includes two profiling positioning blocks arranged opposite to each other on the left and right, and a positioning sensor. The overturn table of the present invention can replace manual overturning workpieces during detection by the automobile sensor, so as to facilitate subsequent detection, and can overturn multiple workpieces at one time to improve work efficiency.

The invention discloses a helium detector for an automobile storage battery box. The detector comprises a table frame, a working part is arranged on the table frame, the working part comprises a mounting plate, the mounting plate is fixedly mounted on the table frame, and a front-back transplanting device, a vacuum box and a leakage detection device are arranged at the top of the mounting plate. The front-back transplanting device is used for installing and moving a storage battery box, the vacuum box is used for fixing and blocking the storage battery box, and the leakage detection device isused for detecting whether the storage battery box leaks air or not. Through arrangement of the front-back transplanting devices, the vacuum box and the leakage detection device, movement, sealing, vacuumizing, helium filling, helium leakage detection and the like of the storage battery box can be realized, so that the airtightness detection of the battery box is realized; meanwhile, helium is used as detection gas, so that the detection is more sensitive and accurate.

The invention discloses a microbiological detection device and a microbiological detection method. The device comprises a cover plate, a substrate I, a substrate II and a bottom plate which are fixedly connected in sequence from top to bottom, and a sample inlet is formed in one end of the cover plate; the substrate I is provided with a microfluid channel I and a microorganism chamber which are communicated with each other, and the microfluid channel I is communicated with the sample inlet; the substrate II is provided with a separation chamber, a microfluid channel II and a reaction chamber which are communicated with one another, a microorganism separation membrane is fixedly connected between the microorganism chamber and the separation chamber, and a microelectrode I is fixedly connected in the reaction chamber; a buffer cavity is formed in the bottom plate, a sensitive element is fixedly connected between the buffer cavity and the reaction cavity, a microelectrode II is fixedly connected in the buffer cavity, a buffer solution inlet and a buffer solution outlet are formed in the two sides of the buffer cavity respectively, and the buffer solution inlet is formed in the lower portion of the reaction cavity. The device can effectively solve the problems that an existing device is low in sensitivity and complex and time-consuming in operation.

The invention discloses a sampling and digestion device for detecting heavy metal in soil. The device comprises a first shell body, a second shell body and a third shell body; a connecting plate is arranged at the bottom of the first shell body and arranged leftward and obliquely, and two round holes are formed in the connecting plate; the second shell body is located on the left side of the lowerportion of the first shell body; the third shell body is located on the right side of the lower portion of the first shell body; the sampling and digestion device for detecting heavy metal in soil issimple in structure and convenient to use, and can conduct pre-treatment on soil under the non-laboratory condition and timely digest sampled soil, a liquid sample is acquired, later detection is convenient, the detection efficiency is improved, and the device is suitable for detection of large-batch soil.

The invention discloses equipment for detecting terminal electrical performance, and belongs to the technical field of transportation detection. The equipment specifically comprises a feeding device which is used for transferring materials, a dislocating device which is arranged at the tail end of the feeding device and is used for distributing materials to each downlink conveying channel, a detecting device which is arranged at the side edge of a detecting sliding rail for abutting joint the tail ends of the downlink conveying channels and is used for pressing down to the materials for detecting electrically, and a material leakage device which is arranged on the detecting sliding rail and is used for pushing out waste materials on the side edge. The equipment for detecting the terminal electrical performance is used for realizing reasonable optimization of pipeline distribution so as to achieve efficient transportation of detecting products and reasonable waste discharge.

The invention discloses an automatic detection device for a buzzer of a single-phase electric energy meter. The device comprises a rack. The rack is provided with one or more tools for the meter positions. The at least one or more tools for the meter positions is provided with a plurality of meter positions. A buzzer detection unit is arranged on the rack above the meter position tool wherein the buzzer detection unit comprises an upper-and-lower straight line driving mechanism on the rack. The lower end of the upper-and-lower straight line driving mechanism is provided with a sound silencing cover. The bottom of the sound silencing cover is provided with one or more cavities to hold the electric energy meters on the various meter position tools. Each cavity is provided therein with a decibel meter and comprises a host computer, a serial server and a monitoring module. The automatic detection device for the buzzer of single-phase electric energy meter can accurately determine whether the sound quality of the buzzer meets the requirement or not.

The invention provides a sample storage device and a sample system. The technical problems that a sample storage device in the prior art needs to be unfrozen repeatedly in the using process, and thus effective ingredients of samples are degraded and cannot be used repeatedly can be solved. The sample storage device comprises a box body, a cover body and at least two independent storage units. The storage units comprise containing cavities used for storing the samples. The box body is provided with a containing space and an opening communicating with the containing space. The cover body is used for opening or closing the opening. The storage units can enter or exit out of the containing space through the opening. When the storage units are located in the storage space, the at least two storage units are sequentially arranged, and every two adjacent storage units are detachably connected. The sample storage device is used for storing detection samples.

The invention provides a detection reagent for a wading pipe as well as a preparation method and a detection method of the detection reagent, and relates to the technical field of pipe detection. Thedetection reagent comprises the following components: a sodium bicarbonate solution, a calcium chloride solution, a sodium hypochlorite solution and pure water. A tap water environment is simulated through the reagent, so that toxic substances contained in the pipe are extracted and analyzed, and the accuracy of a detection result is improved. In addition, the invention also provides a method forpreparing the detection reagent for the wading pipe. The method comprises the following steps: preparing a sodium hypochlorite solution, preparing a sodium bicarbonate solution, preparing a calcium chloride solution and preparing the detection reagent. The method is simple in process, and the prepared reagent is good in using effect. The invention further provides a detection method of the wadingpipe. The detection method comprises the following steps of pipe treatment, pipe soaking, blank test, soaking liquid collection and detection. The method is simple in process and accurate and reliablein analysis result.

The invention relates to a target species specific sequence acquisition method and a target species detection method; the target species specific sequence acquisition method comprises the following steps: selecting all whole genome sequences of a target species in a genome database, and screening out a target species core sequence by comparing all whole genome sequences of the target species; filtering out all nucleic acid sequences of the target species in a nucleic acid database to obtain a non-target species nucleic acid sequence database; and comparing the core sequence of the target species with a non-target species nucleic acid sequence database, and filtering out the nucleic acid sequence obtained by comparison in the core sequence of the target species to finally obtain a specificsequence of the target species. By means of the method, the specific sequence of the target species can be obtained, the method is simple and high in reliability, and the target species and the classification status of the organism can be determined by identifying whether the specific sequence of the target species exists in the sample or not. Therefore, the existence of the target species or theclassification status of the target species can be identified only by amplifying the specific sequence of the target species obtained by the method for obtaining the specific sequence of the target species.

The invention discloses a particulate matter collection device and detection method for incense burning products. The particulate matter collection device comprises a detection cylinder, an upper cover, a sampling head and a supporting net, a first door body is arranged on a sample adding opening, and a first sealing strip is arranged on the first door body; an ash removal opening and an air inlet are formed in the side wall of the detection cylinder below the supporting net, a second door body is arranged on the ash removal opening, and a second sealing strip is arranged on the second door body; the upper cover covers the upper end of the detection cylinder in a sealing manner, and the sampling head is arranged in the middle of the upper cover and is positioned at the upper end of an inner cavity defined by the detection cylinder and the upper cover. Compared with the prior art, the incense burning products are placed in the detection cylinder, particulate matter in air is filtered through filter paper, the gap between the first door body and the second door body is blocked through the first sealing strip and the second sealing strip, and generated smoke is prevented from being diffused outwards through the gap as much as possible; and therefore, the detection effect is improved.