Liquid drop digital PCR chip and liquid drop digital PCR device

Abstract

The invention provides a liquid drop digital PCR chip, which comprises a chip body. One or more liquid drop generation units are arranged on the chip body and each comprise a liquid injection pond, aliquid drop generation cavity, a liquid drop flat-spreading cavity and an oil injection pond which communicate sequentially, after the liquid drop generation units are fully filled with oil phase reagents, PCR reagents flow out from the liquid drop generation cavities through the liquid injection ponds, then a plurality of liquid drops are formed, and the multiple liquid drops are flat spread in the liquid drop flat-spreading cavities in a single-layer mode. The liquid drop digital PCR chip integrates liquid drop generation and single-layer liquid drop flat-spreading functions, the chip is directly placed in a PCR instrument, thus the liquid drops in the chip can be subjected to PCR reaction, the generated liquid drops do not need to be transferred again, the state of the liquid drops is better maintained, and PCR is facilitated; and the liquid drops are flat spread in thea single-layer mode, the liquid drops are not overlapped, subsequent detection is convenient, the detection efficiency is improved, and the detection accuracy rate is increased.

Description

technical field [0001] The invention relates to the technical field of biological chips, in particular to a droplet digital PCR chip and a droplet digital PCR device. Background technique [0002] Droplet digital PCR uses a microfluidic chip to process samples into droplets before traditional PCR amplification, that is, the reaction system containing nucleic acid molecules is divided into tens of thousands of micro-droplets of nanoliters, and each micro-droplet or Does not contain the nucleic acid target molecule to be detected, or contains the nucleic acid target molecule to be detected. After PCR amplification, each droplet is detected one by one. The droplet with fluorescent signal is interpreted as 1, and the droplet without fluorescent signal is interpreted as 0. According to the principle of Poisson distribution and the number and proportion of positive droplets, The starting copy number or concentration of the target molecule can be derived. Each chip can generate m...

AI Technical Summary

Problems solved by technology

In this process, the generated micro-droplets need to be transferred, which is likely to damage the size and internal environment of the micro-droplets. At the same time, the operation requirements of the operator are high, and it takes time and effort; secondly, the droplets are stacked in the 96-well plate In the follow-up detection, it is necessary to separately separate the droplets for fluorescence detection; and, in the PCR process, long-term heating is required, and the oil phase is easy to generate bubbles during the heating process, which will destroy the droplet structure and cause detection failure.

Images