Target species specific sequence acquisition method and target species detection method

Abstract

The invention relates to a target species specific sequence acquisition method and a target species detection method; the target species specific sequence acquisition method comprises the following steps: selecting all whole genome sequences of a target species in a genome database, and screening out a target species core sequence by comparing all whole genome sequences of the target species; filtering out all nucleic acid sequences of the target species in a nucleic acid database to obtain a non-target species nucleic acid sequence database; and comparing the core sequence of the target species with a non-target species nucleic acid sequence database, and filtering out the nucleic acid sequence obtained by comparison in the core sequence of the target species to finally obtain a specificsequence of the target species. By means of the method, the specific sequence of the target species can be obtained, the method is simple and high in reliability, and the target species and the classification status of the organism can be determined by identifying whether the specific sequence of the target species exists in the sample or not. Therefore, the existence of the target species or theclassification status of the target species can be identified only by amplifying the specific sequence of the target species obtained by the method for obtaining the specific sequence of the target species.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for obtaining a specific sequence of a target species and a method for detecting the target species. Background technique [0002] Traditional species identification methods include (1) identification of species based on 16s (bacteria), 18S (higher eukaryotes) or ITS (fungi) and key genes (viruses): by obtaining the DNA of the target species, performing PCR amplification, and performing Sanger For sequencing, submit the sequence to online blastn for sequence comparison. Based on sequence similarity, the taxonomic status of the species is determined. The detection efficiency of this detection method is low, and the DNA of a single species needs to be purified, and the taxonomic status of the species needs to be sequenced and compared; based on this method, the resolution is low, and many species cannot be determined, but can only be identified to the genus or family. (2) I...

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: First, in order to ensure the comprehensiveness of the species as much as possible, the sequencing depth is high, the sequencing cost is high, and the subsequent assembly and analysis have high requirements on the computing platform, a large amount of calculation, and a long time. Unable to meet follow-up analysis requirements

Second, there may be homologous fragments between different species, and a metagenomic assembly fragment may have multiple different taxonomic statuses, which may cause uncertainty and affect the accuracy of species identification

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