Rabdosia amethystoides tissue culture and rapid propagation method

Abstract

The invention discloses a rabdosia amethystoides tissue culture and rapid propagation method. Rabdosia amethystoides belongs to a perennial herb, is distributed in most provinces in China and mostly grown on hillsides, sandy land and ravines, tastes bitter, is slightly cold in nature, and has the effects of clearing heat, detoxicating, invigorating stomach, invigorating the blood circulation, eliminating phlegm and the like. At present, seedling growing of the rabdosia amethystoides is carried out mainly through traditional modes such as cuttage, grafting and sowing, and the shortcomings thatthe seedling growing cost is high, seedling growth is poor, seedlings are uneven, and improved variety potential cannot be fully exerted exist. Excellent clonal twigs serve as explants, in-vitro replanted plants of the rabdosia amethystoides are obtained through the processes such as explant disinfection, induction culture, cluster bud propagation, adventitious rooting, seedling exercising and transplanting, a tissue culture and rapid propagation technique system of the rabdosia amethystoides is established, the excellent characteristics of the clonal female parent of the rabdosia amethystoides can be maintained, and large-scale production of the excellent clonal seedlings of the rabdosia amethystoides is facilitated.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of fragrant tea vegetables. Background technique [0002] Fragrant tea is a perennial herb, distributed in most provinces of our country, and mostly grows on hillsides, sandy ground, and ravines. Bitter in the mouth, slightly cold in nature. It has the functions of clearing away heat and detoxification, invigorating the stomach, promoting blood circulation and eliminating phlegm. Fragrant tea is a unique plant species in southern my country. It grows fast and is one of the most important commercial medicinal materials in southern my country. In recent years, with the advancement of the reform of the collective forestry and animal husbandry system and the increase in the price of commercial materials, forest farmers have become more active in managing fragrant tea and vegetables, and their manage...

AI Technical Summary

Problems solved by technology

At present, traditional methods such as cuttings, grafting and sowing are mainly used to raise seedlings. There are disadvantages such as high seedling cost, poor growth of seedlings, uneven growth, and failure to fully develop the potential of improved seeds, making the current supply of high-quality strong seedlings unable to meet the market. needs