Rabdosia amethystoides tissue culture and rapid propagation method

A technology of tissue culture rapid propagation and fragrant tea vegetables, applied in the field of plant tissue culture, can solve the problems of high cost of seedling breeding, failure to fully realize the potential of improved seeds, unevenness, etc.

Inactive Publication Date: 2019-02-22
钟天路
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, traditional methods such as cuttings, grafting and sowing are mainly used to raise seedlings. There are disadvantages such as high seedling cost, poor growth of see

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Collection and disinfection of explants: take as explants plump young shoots in the sprouting state that have the same morphological characteristics at the base of the fragrant tea, wash them under running water for 3 hours, soak them in 3% washing powder solution for 6 minutes, and wash them with soft hair. Brush the material gently with 3% washing powder solution, then rinse with tap water in the form of dripping water for 61 minutes, rinse with distilled water for 4 times, soak in 75% ethanol solution for 21 seconds in an ultra-clean workbench, rinse with sterile water for 6 times, and then rinse with Disinfect with 0.01% Tween-20 and 0.1% mercuric chloride solution for 6 minutes, rinse with sterile water for 4 times, and dry the surface with sterile filter paper for later use.

[0015] (2) Induction culture: Cut the shoots treated in step (1) into stems of about 1.6 cm in length and inoculate them into induction medium for cluster bud induction culture. After in...

Embodiment 2

[0020] (1) Collection and disinfection of explants: take as explants plump twigs in the budding state that are consistent with the morphology of the base of the fragrant tea and vegetable stumps, wash them under running water for 5 hours, soak them in 3% washing powder solution for 10 minutes, and wash them with soft water. Gently scrub the material with a 4% washing powder solution with a brush, then rinse with tap water in the form of dripping water for 72 minutes, rinse with distilled water for 6 times, soak in 78% ethanol solution for 32 seconds in an ultra-clean workbench, rinse with sterile water for 9 times, and then use Disinfect with 0.1% mercuric chloride solution containing 0.01% Tween-20 for 7 minutes, rinse with sterile water for 7 times, and dry the surface with sterile filter paper for later use.

[0021] (2) Induction culture: The shoots treated in step (1) were cut into stems of about 1.7 cm and inoculated into induction medium for cluster bud induction culture...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a rabdosia amethystoides tissue culture and rapid propagation method. Rabdosia amethystoides belongs to a perennial herb, is distributed in most provinces in China and mostly grown on hillsides, sandy land and ravines, tastes bitter, is slightly cold in nature, and has the effects of clearing heat, detoxicating, invigorating stomach, invigorating the blood circulation, eliminating phlegm and the like. At present, seedling growing of the rabdosia amethystoides is carried out mainly through traditional modes such as cuttage, grafting and sowing, and the shortcomings thatthe seedling growing cost is high, seedling growth is poor, seedlings are uneven, and improved variety potential cannot be fully exerted exist. Excellent clonal twigs serve as explants, in-vitro replanted plants of the rabdosia amethystoides are obtained through the processes such as explant disinfection, induction culture, cluster bud propagation, adventitious rooting, seedling exercising and transplanting, a tissue culture and rapid propagation technique system of the rabdosia amethystoides is established, the excellent characteristics of the clonal female parent of the rabdosia amethystoides can be maintained, and large-scale production of the excellent clonal seedlings of the rabdosia amethystoides is facilitated.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of fragrant tea vegetables. Background technique [0002] Fragrant tea is a perennial herb, distributed in most provinces of our country, and mostly grows on hillsides, sandy ground, and ravines. Bitter in the mouth, slightly cold in nature. It has the functions of clearing away heat and detoxification, invigorating the stomach, promoting blood circulation and eliminating phlegm. Fragrant tea is a unique plant species in southern my country. It grows fast and is one of the most important commercial medicinal materials in southern my country. In recent years, with the advancement of the reform of the collective forestry and animal husbandry system and the increase in the price of commercial materials, forest farmers have become more active in managing fragrant tea and vegetables, and their manage...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00A01G31/00
CPCA01G31/00A01H4/001A01H4/005A01H4/008
Inventor 钟天路
Owner 钟天路
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap