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Aggregation-induced luminescent ultrathin lysozyme fluorescent nanofilm and its anti-counterfeiting application

An aggregation-induced luminescence and fluorescent nanotechnology, applied in the field of aggregation-induced luminescence ultra-thin lysozyme fluorescent nano-films, can solve the problems of coating stability, complex manufacturing process, biological safety, etc., and achieve easy operation, simple operation, low cost effect

Active Publication Date: 2020-07-28
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these strategies still face many challenges, such as biosafety issues, difficulty in forming stable coatings on the surface of various commodity materials and coating stability issues, high cost, complex manufacturing processes, and concerns for end consumers. complex laboratory analysis

Method used

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  • Aggregation-induced luminescent ultrathin lysozyme fluorescent nanofilm and its anti-counterfeiting application
  • Aggregation-induced luminescent ultrathin lysozyme fluorescent nanofilm and its anti-counterfeiting application
  • Aggregation-induced luminescent ultrathin lysozyme fluorescent nanofilm and its anti-counterfeiting application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Mix 100 μL 2 mg / mL lysozyme aqueous solution and 20 μL 0.1 mg / mL 9,10-distyryl anthracene aqueous solution, and record it as liquid A; adjust 50 mmol / L tris(2-carboxyethyl)phosphine aqueous solution with NaOH to The pH value is 7.0, which is recorded as solution B; mix 100μLA solution and 100μL B solution evenly, float a transparent glass piece on the surface of the resulting mixture, and incubate at room temperature for 30 minutes, forming a layer of aggregation-induced luminescent ultra-thin lysate on the transparent glass surface Enzyme fluorescent nanofilms.

[0027] The light transmittance of the fluorescent nanofilm was characterized ( figure 1 ), fluorescence and optical characterization ( figure 2 ), plan and cross-sectional characterization of scanning electron microscopy ( image 3 ), morphology characterization by atomic force microscopy ( Figure 4 ), and use 340W xenon lamp irradiation for 30 minutes to detect its photobleaching resistance ( Figure 5 ...

Embodiment 2

[0031] Application of Aggregation-Induced Luminescent Ultrathin Lysozyme Fluorescent Nanofilm as Anti-counterfeiting Coating

[0032] Mix 100 μL of 2 mg / mL lysozyme aqueous solution and 20 μL of 0.1 mg / mL 9,10-distyryl anthracene (DSAI) aqueous solution, and record it as liquid A; Adjust the pH value to 7.0 with NaOH, and record it as solution B; mix 100μLA solution and 100μL B solution evenly, and immerse substrates such as silicon wafers, glass wafers, gold wafers, conductive glass, polyester, polycarbonate, and plexiglass in the obtained The mixed solution is incubated at room temperature for 30 minutes to form a layer of aggregation-induced luminescent ultrathin lysozyme fluorescent nano film on the surface of the substrate. The contact angle test was carried out on the surface of the substrate before and after treatment, and the results are shown in Figure 8 . The results show that the fluorescent nano film can be adhered to various substrate surfaces.

[0033] Furthe...

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Abstract

The invention discloses an aggregation-induced emission ultrathin lysozyme fluorescent nano film and an antifake application thereof. Water soluble aggregation-induced emission molecules take part ina phase transformation self-assembling process of lysozyme to obtain the aggregation-induced emission ultrathin lysozyme fluorescent nano film through a simple one-step self assembly. The fluorescentnano film has good stability, adhesion, biocompatibility and excellent optical transparency, also has a fluorescent property, can be stably adhered to the surfaces of various matrixes, is suitable forantifake demands of various commodities, has wide universality, is simple and efficient in preparation process, low in cost, green and environment-friendly and easy to achieve industrial applicationson a large scale.

Description

technical field [0001] The invention relates to an environmentally friendly, simple, efficient and widely applicable aggregation-induced luminescent ultra-thin lysozyme fluorescent nano film and its application as an anti-counterfeiting coating. Background technique [0002] The counterfeiting of valuable documents, currencies and branded products is a growing global challenge with serious economic, safety and health implications for governments, businesses, corporations and consumers around the world, necessitating a high volume of anti-counterfeiting labels . In particular, counterfeit and shoddy behaviors in the fields of medicine and food seriously endanger human health. So far, anti-counterfeiting technologies such as security inks, luminescent nanomaterials, quantum dots, metal organic frameworks, electrospun fibers, photonic crystals, and nanoprinting have been widely used in various commercial fields such as currency, pharmaceuticals, microelectronics, automobiles, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D189/00C09D7/63C09D5/22
CPCC09D5/22C09D7/63C09D189/00C08K5/01C08K5/50
Inventor 杨鹏刘瑞瑞
Owner SHAANXI NORMAL UNIV
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