Health-care food functional ingredient detecting method

A technology of health food and detection method, applied in the field of food analysis, can solve problems such as inability to treat diseases, and achieve the effects of no complicated steps, easy popularization and application, and simple method.

Inactive Publication Date: 2019-02-22
遵义市精科信检测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Health food is a type of food, which has the commonality of general food, can regulate the functions of the human body, and is suitable for specific groups of people, but it cannot treat diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] 1. Drawing of standard curve

[0012] To prepare dextran standard stock solution, accurately weigh 0.5007 g of dextran reference substance dried to constant weight, add water to dissolve, and dilute to 50 ml, mix well, and store in the refrigerator.

[0013] Dextran standard use solution preparation, draw 1.00ml of dextran standard stock solution, put it in a 100ml measuring bottle, add water to the mark, mix well, and store in the refrigerator. This solution contains 0.10mg of dextran per 1ml.

[0014] Precisely draw 0, 0.10, 0.20, 0.40, 0.60, 0.80, 1.00ml of dextran standard solution (equivalent to 0, 0.010, 0.020, 0.040, 0.060, 0.080, 0.10mg of dextran) into 25ml colorimetric tubes Add water to 2.0ml accurately, add 1.0ml of 50g / L phenol solution, mix on a rotary mixer, carefully add 10.0ml of concentrated sulfuric acid, mix carefully on a rotary mixer, and boil in a boiling water bath for 2min , After cooling to room temperature, use a spectrophotometer to measure...

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PUM

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Abstract

The invention relates to a health-care food functional ingredient detecting method. Polysaccharide is a non-specific immunological enhancement compound, the content of polysaccharide is determined bya phenol-sulfuric acid colorimetric method, the method is stable, and appropriately, the glucose concentration is controlled to be 510-2510 mg/L. A phenol reagent and concentrated sulfuric acid are added precisely, shaking is performed after standing, heating is performed in a water bath for 15 minutes, then instant cooling is performed, and it can be seen through linearity, precision, stability and other testing results that a spectrophotometric method for determining the content of crude polysaccharide in a health-care food is high in accuracy, good in repeatability, simple and easy to operate.

Description

technical field [0001] The invention belongs to the field of food analysis, in particular to a method for detecting functional components of health food. Background technique [0002] Health food is a category of food, which has the commonality of general food, can regulate the functions of the human body, and is suitable for specific groups of people, but cannot treat diseases. The rise of my country's health (functional) food was in the late 1980s and early 1990s. After the development of the first and second generations, it will also enter the third generation, that is, health food not only requires human and animal experiments to prove that the product has certain Physiological regulation function, it is necessary to find out the structure, content, mechanism of action and stable form in food of the factor with this health function. [0003] The functional components of health food mainly include polysaccharides, saponins, flavonoids, enzyme hormones, endogenous substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 陶远波
Owner 遵义市精科信检测有限公司
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