A micro-cell culture device and method for controlling the internal environment of the cavity

Abstract

The invention discloses a micro-cell culture device that can be placed on an inverted microscope and a control method for the internal environment of the cavity thereof. The micro-cell culture device includes a heating constant temperature unit, a circulating gas humidification unit, a CO 2 Circulation control unit and display and control unit; the control method of the internal environment of the cavity is through temperature, humidity and CO 2 The concentration control step precisely controls the internal environment of the chamber; the micro-cell culture device can be used in conjunction with experimental platforms such as microscopes, breaking the limitations of conventional cell culture incubators on subsequent experimental conditions, and has a wide range of applications; the device utilizes sensing and PID Control technology to precisely control the temperature, humidity and CO in the microcavity 2 The concentration can provide cells with an incubation environment suitable for growth, and can efficiently and stably maintain the required external environment to ensure the proliferation and vitality of cells.

Description

technical field [0001] The invention relates to the field of cell culture devices, in particular to a miniature cell culture device and a method for controlling the internal environment of a cavity thereof. Background technique [0002] In vitro cell culture technology refers to the use of artificial methods to provide nutrients for cell growth after the cells are isolated from the body, simulating the external environment required for cell growth, so as to achieve continuous and healthy growth, reproduction, and maintenance of the functions and morphological properties of the isolated cells. The goal. In the process of cell culture, maintaining cell viability must first maintain a stable extracellular environment. Currently in the laboratory, CO is usually used 2 The cell incubator provides a stable and efficient culture environment for cell culture. [0003] However, conventional CO 2 The cell incubator has a large volume and cannot be combined with other experimental ...

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Problems solved by technology

[0003] However, conventional CO 2 The cell incubator has a large volume and cannot be combined with other experimental instruments; therefore, in the existing experimental design, although the CO 2 The cell incubator can ensure the viability of cells in cell culture, but in other processes of cell research, it often cannot provide a stable and efficient extracellular environment for cell incubation and growth

Therefore, the existing cell culture device needs to separate cell culture from other follow-up experiments, which brings a series of problems, such as: separation of cell culture and cell operation, inability to guarantee cell viability, high risk of bacterial infection, repeated samples, observation Narrow windows, inconsistent cell controls, etc.

These disadvantages are unavoidable under the existing experimental conditions

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