Minisize cell culture device, and cavity internal environment control method thereof

Abstract

The invention discloses a minisize cell culture device which can be placed on an inverted microscope, and a cavity internal temperature control method thereof. The minisize cell culture device comprises a heating constant temperature unit, a circulation gas humidifying unit, a CO2 circulation control unit, and a display and control unit. According to the cavity internal temperature control method,temperature, humidity, and CO2 concentration controlling steps are adopted for accurate control of the cavity internal environment. The minisize cell culture device can be combined with experiment platforms such as microscopes, the limits of routine cell culture boxes on subsequent experiment conditions are broken, and the application range is wide. According to the minisize cell culture device sensing and PID control technology are adopted, so that accurate controlling on micro-cavity internal temperature, humidity, and CO2 concentration is realized, a culture environment suitable for cell growth is provided for cells, the needed external environment can be maintained steadily with high efficiency, and cell proliferation vigour is ensured.

Description

technical field [0001] The invention relates to the field of cell culture devices, in particular to a miniature cell culture device and a method for controlling the internal environment of a cavity thereof. Background technique [0002] In vitro cell culture technology refers to the use of artificial methods to provide nutrients for cell growth after the cells are isolated from the body, simulating the external environment required for cell growth, so as to achieve continuous and healthy growth, reproduction, and maintenance of the functions and morphological properties of the isolated cells. The goal. In the process of cell culture, maintaining cell viability must first maintain a stable extracellular environment. Currently in the laboratory, CO is usually used 2 The cell incubator provides a stable and efficient culture environment for cell culture. [0003] However, conventional CO 2 The cell incubator has a large volume and cannot be combined with other experimental ...

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Problems solved by technology

[0003] However, conventional CO 2 The cell incubator has a large volume and cannot be combined with other experimental instruments; therefore, in the existing experimental design, although the CO 2 The cell incubator can ensure the viability of cells in cell culture, but in other processes of cell research, it often cannot provide a stable and efficient extracellular environment for cell incubation and growth

Therefore, the existing cell culture device needs to separate cell culture from other follow-up experiments, which brings a series of problems, such as: separation of cell culture and cell operation, inability to guarantee cell viability, high risk of bacterial infection, repeated samples, observation Narrow windows, inconsistent cell controls, etc.

These disadvantages are unavoidable under the existing experimental conditions

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