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Preparation method for chlorella immunologic competent peptide

A technology of immunoactive peptides and chlorella, applied in the field of preparation of chlorella immunoactive peptides, can solve problems such as restricting the medical value of chlorella, achieve good cell proliferation and phagocytosis, improve extraction rate, and promote delayed onset Allergic Effects

Active Publication Date: 2019-03-01
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no relevant research to prove what kind of components are the main substances that exert high biological activity in Chlorella, and what are the biological activities and functions of different components
Obviously, these research deficiencies have seriously restricted the further development of the medical value of chlorella, especially the development of high-value chlorella health products

Method used

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  • Preparation method for chlorella immunologic competent peptide
  • Preparation method for chlorella immunologic competent peptide
  • Preparation method for chlorella immunologic competent peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Preparation of Chlorella cell wall enzymatic hydrolyzate and specific protein enzymatic hydrolyzate

[0030] 1) The composition ratio of the chlorella cell wall enzymatic hydrolyzate is:

[0031]

[0032] The pH was adjusted to 4.0 with concentrated hydrochloric acid with a mass fraction of 36%.

[0033] 2) The proportioning of the specific proteolytic solution components is:

[0034]

[0035] Adjust the pH to 2.5 with a concentrated hydrochloric acid solution with a mass fraction of 37%;

[0036] 2. Chlorella immunoactive peptides are prepared by the following steps:

[0037] 1) Weigh 500g of chlorella powder and add it to 4.5L of 0.3mol / L NaOH dilute alkali solution, and soak for 60 min at 60°C to obtain the pretreated chlorella liquid;

[0038] 2) The pretreated chlorella liquid obtained in step 1) was homogenized under high pressure for 3 times under 45MPa pressure, centrifuged at 5000rpm for 20min, and the obtained supernatant A was temporarily stored at ...

Embodiment 2

[0067] 1. Preparation of Chlorella cell wall enzymatic hydrolyzate and specific protein enzymatic hydrolyzate

[0068] The cellulase, pectinase and various proteases used were purchased from Beijing Suolaibao Technology Co., Ltd.

[0069] 1) The weight ratio of the chlorella cell wall enzymatic hydrolyzate is:

[0070]

[0071] Adjust the pH to 4.0 with concentrated hydrochloric acid with a mass fraction of 36%;

[0072] 2) The weight ratio of the specific proteolytic solution is:

[0073]

[0074] Adjust the pH to 2.0 with a concentrated hydrochloric acid solution with a mass fraction of 37%;

[0075] 2. Prepared by the following steps:

[0076] 1) Weigh 750g of chlorella powder and add it to 4.25L of 0.3mol / L NaOH dilute alkali solution, and soak for 60min at 60°C to obtain the pretreated chlorella liquid;

[0077] 2) The pretreated chlorella liquid obtained in step 1) was homogenized under high pressure twice under a pressure of 55MPa, centrifuged at 5000rpm for 20m...

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Abstract

The invention provides a preparation method for a chlorella immunologic competent peptide. The preparation method is characterized by following steps: adopting a high pressure homogenization method and cellulase / pectinase enzymolysis for extracting chlorella protein, adopting specific proteolysis liquor for enzymolysis, and then separating with an ultrafiltration film, thereby acquiring chlorellaenzymolysis peptides with different molecular weights. According to the invention, compared with other protease enzymatic hydrolysate and polypeptide products with other molecular weight after adopting the same specific proteolysis liquor for enzymolysis, the chlorella peptide with molecular weight of 0-1kDa after adopting specific proteolysis liquor for enzymolysis has better in vitro macrophagemultiplication and phagocytic ability and better capacity for boosting mice delayed hypersensitivity and mice in vivo lymphocyte multiplication and transformation. According to the invention, the preparation for chlorella bioactive peptide with better function of boosting immunity depends on the specific proteolysis liquor, and the development and utilization of immunity-boosting functional healthfood and medicine products are benefited.

Description

technical field [0001] The invention provides a preparation method of chlorella immunoactive peptide and belongs to the field of biotechnology. Background technique [0002] Chlorella (Chlorella) is a universal single-celled green algae, which is widely distributed in nature. It can grow and reproduce by using organic carbon sources under heterotrophic conditions. Creatures that grow 4x per hour. Chlorella is extremely nutritious, in which the crude protein content is as high as 50%, the composition of essential amino acids is basically balanced, and it also contains unsaturated fatty acids, biologically active polysaccharides, nucleic acids, vitamins, trace elements, minerals, chlorophyll, etc., with extremely high nutritional value , has various biopharmacological activities such as preventing and treating peptic ulcer, anti-tumor, enhancing immunity, anti-radiation, anti-pathogenic microorganisms, preventing and treating anemia, lowering blood lipid and anti-atherosclero...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06Y02P20/10
Inventor 林陈胜张彦定
Owner FUJIAN NORMAL UNIV
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