Ammonia nitrogen removal bacterium agent

A technology for removing bacteria and ammonia nitrogen, which is applied in the fields of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc. It can solve the problems of long film hanging time of biological filter, low ammonia nitrogen removal rate, and large temperature limitation. , to achieve the effect of strong adaptability, firmer bacteria, and low accumulation.

Inactive Publication Date: 2019-03-08
广州小众环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological nitrification denitrification technology has many defects such as large temperature limitation, long time for biofilter to hang membrane, high energy consumption for oxygenation, and low ammonia nitrogen removal rate. These shortcomings have seriously restricted the promotion and development of recirculating aquaculture systems. effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A kind of ammonia nitrogen bacteria removal agent provided by the present invention is prepared by the following steps: take 5g composite bacteria agent, 80g filling, mix and stir 50g polyvinyl alcohol and 800ml water, and mix and stir in a mixer whose stirring speed is 100r / min Mix for 30 minutes, add 20g sodium alginate and 320ml 5% calcium chloride solution to granulate and coat.

[0032] The mass ratio of Halomonas sativa, Bacillus subtilis and Rhodococcus erythropolis is 4:3:3.

[0033] The preparation method of the compound bacterial agent is to first inoculate 4% Halomonas sativa and 3% Rhodococcus erythropolis to the mixed culture medium, place it on a shaker at 30°C and 200r / min for 40 hours, and then inoculate 3% Subtilis subtilis Bacillus, continue the mixed culture until 57h; take the mixed fermentation broth, centrifuge at 6000r / min for 5min, add 0.8% sterile physiological saline which is 1 / 5 of the volume of the mixed fermentation broth before centrifugati...

Embodiment 2

[0041] A kind of ammonia nitrogen bacteria removal agent provided by the present invention is prepared by the following steps: take 8g composite bacteria agent, 60g filling, mix and stir 80g polyvinyl alcohol and 500ml water, and mix and stir in a mixer whose stirring speed is 200r / min Mix for 5 minutes, add 32g sodium alginate and 200ml 5% calcium chloride solution to granulate and coat.

[0042] The mass ratio of the Halomonas salina, Bacillus licheniformis and Pseudomonas stutzeri is 4:3:3.

[0043] The preparation method of the composite microbial agent is to first inoculate 4% Halomonas sativa and 3% Pseudomonas stutzeri to the mixed culture medium, place it at 30°C and cultivate it on a shaker at 200r / min for 48h, and then inoculate it for 3 % Bacillus licheniformis, continue the mixed culture until 57-62h; take the mixed fermentation broth, centrifuge at 6000r / min for 5min, add 0.8%-0.9% of the volume of the mixed fermentation broth before centrifugation to resuspend in...

Embodiment 3

[0051] A kind of ammonia nitrogen bacteria removal agent provided by the present invention is prepared by the following steps: take 6g composite bacteria agent, 70g filling, mix and stir 70g polyvinyl alcohol and 600ml water, and mix and stir in a mixer whose stirring speed is 120r / min Mix for 20 minutes, add 25g sodium alginate and 280ml 5% calcium chloride solution to granulate, then add saturated boric acid solution to harden for 24 hours to obtain solidified granules, rinse with normal saline for 3 times, collect, and store in a refrigerator at 4°C.

[0052] The mass ratio of Halomonas sativa, Bacillus licheniformis and Rhodococcus erythropolis is 4:3:3.

[0053] The preparation method of the compound bacterial agent is to first inoculate 4% Halomonas yantiana and 3% Rhodococcus erythropolis or Pseudomonas stutzeri to inoculate the mixed culture medium, and culture it on a shaker at 30°C and 200r / min. 40-48 hours, then inoculate with 3% Bacillus subtilis or Bacillus lichen...

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Abstract

The invention belongs to the technical field of sewage treatment, discloses an ammonia nitrogen removal bacterium agent, and aims to provide an ammonia nitrogen removal bacterium agent which is high in ammonia nitrogen removal efficiency and can recycle bacterium removal agents and fillers. The technical scheme includes that compound bacterium agents are cured on the fillers and comprise heterotrophic nitrification bacteria, bacillus and denitrifying bacteria.

Description

technical field [0001] The invention discloses an ammonia nitrogen bacteria removal agent, and also discloses a preparation method of the ammonia nitrogen bacteria removal agent, which belongs to the technical field of sewage treatment. Background technique [0002] Ammonia nitrogen exists in many industrial wastewaters. Ammonia nitrogen discharged into water bodies, especially lakes and bays with slow flow, is likely to cause a large number of algae and other microorganisms in the water to form eutrophic pollution. In addition to making the operation of tap water treatment plants difficult, causing drinking In addition to the peculiar smell of the water, in severe cases, the dissolved oxygen in the water will drop, a large number of fish will die, and even the lake will dry up and perish. The traditional biological denitrification method mainly relies on nitrifying bacteria and denitrifying bacteria to gradually convert nitrogenous substances in sewage into nitrate nitrogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/08C12N11/10C02F3/34C12R1/01C12R1/125C12R1/10C12R1/38C02F101/16
CPCC02F3/34C02F3/348C02F2101/16C12N1/20C12N11/08C12N11/10
Inventor 王文杰饶丹叶涛
Owner 广州小众环保科技有限公司
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