A targeting peptide based on subunit b of human calmodulin phosphatase, preparation method and application thereof
A targeting peptide and targeting technology, applied in the field of biomedicine, can solve problems such as increasing the risk of squamous cell carcinoma
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Embodiment 1
[0065] Example 1 Expression of Targeted Peptide Fusion Protein
[0066] 1. Structure design of targeted peptide fusion protein
[0067] The structural schematic diagram of the targeting peptide fusion protein is as follows figure 1 As shown, GFP is located at the N-terminus of the fusion protein, and the full-length CNB or CNB truncations of different lengths are located at the C-terminus of the fusion protein, and the middle is connected with the GGGSGGGS linker. The number of amino acids contained in each CNB truncated body and the distribution of amino acids in the full-length CNB are shown in the figure. The full-length CNB contained by GFP-CNB has the amino acid sequence of positions 1-170 (as shown in SEQ ID NO. 3); the CNB truncated body Trun1 contained by GFP-Trun1 has the amino acid sequence of positions 1-85 of the full-length CNB; The CNB truncated body Trun2 contained by GFP-Trun2 has the amino acid sequence of the 86th-170th position of the full-length CNB; the ...
Embodiment 2
[0075] Example 2 Targeted transport of targeting peptides in animals
[0076] 1. Establishment of tumor animal model
[0077] The purchased Balb / c 5-6-week-old female nude mice were randomly divided into groups and inoculated subcutaneously with 2*10 6 HepG2 tumor cells were placed in the armpits of mice, and when the tumors grew to the size of soybeans, about 200-500mm 3 , the experiment can be performed.
[0078] 2. Fluorescent labeling of targeting peptides
[0079] The cyanine dye Cy7 was selected as the fluorescent substance, and the Trun3 truncated body (which could be chemically synthesized) and the full-length CNB (which could be chemically synthesized) were labeled according to conventional techniques, and the labeled targeting peptide was used for targeting research.
[0080] 3. Real-time fluorescent quantitative detection of targeting peptides
[0081] The mice were divided into 3 groups, the blank control group (1), the positive control CNB-Cy7 (4), and the exp...
Embodiment 3
[0082] Example 3 Targeted delivery of anti-tumor targeted drugs
[0083] According to the experimental results of the previous examples, it can be concluded that Trun3 and Trun6 can be used as targeted transport peptides to bring other substances into tumor cells or tumor tissues. Therefore, antitumor drugs can be linked to Trun3 or Trun6, and they can be injected intravenously, Orally or in other ways, it is introduced into the body of tumor patients, so that anti-tumor drugs can target tumor tissues, specifically kill tumor cells, and reduce the side effects of tumor treatment drugs.
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