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Thiourea degrading strain and method for treating thiourea-containing wastewater by applying same

A technology of thiourea and bacteria strains, applied in the field of environmental microorganisms, can solve the problems of secondary pollution and difficult discharge of wastewater, and achieve the effects of stable effluent quality, avoiding secondary pollution, and low cost

Active Publication Date: 2019-03-15
CHAMBROAD CHEM IND RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the deficiencies in the prior art, the inventor carried out research on microbial degradation of thiourea and release of nitrification inhibition, and finally screened out a thiourea-degrading bacterial strain YJY18-03, which belongs to Bacillus cereus. Applied in the aerobic biochemical system of thiourea-containing wastewater, it can completely degrade thiourea and discharge the ammonia nitrogen up to the standard; avoid the secondary pollution caused by chemical treatment, and the waste water is difficult to discharge up to the standard

Method used

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  • Thiourea degrading strain and method for treating thiourea-containing wastewater by applying same
  • Thiourea degrading strain and method for treating thiourea-containing wastewater by applying same
  • Thiourea degrading strain and method for treating thiourea-containing wastewater by applying same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The composition of the seed medium is by weight (w / w): The composition of the seed medium is by weight (w / w): glucose 1.3%, ammonium sulfate 0.5%, yeast extract powder 1%, magnesium sulfate 0.05%, Potassium dihydrogen phosphate 0.05%, add water to 1000mL, pH7.3~7.4; sterilize at 121℃, 0.15Mpa for 20min;

[0047] The amount of inoculum is to inoculate one test tube slant strain after activation (transfer the test tube slant strain stored on nutrient agar medium at 4°C to room temperature for activation for 4h-8h) per Erlenmeyer flask (100mL culture medium). The culture temperature is 33°C, the shaker speed is 200r / min, the culture time is 12h, and the seed solution OD 600 is 0.7;

[0048] Submerged fermentation medium composition, by weight (w / w): corn flour 1.4%, soybean meal 2%, ammonium nitrate 0.4%, manganese sulfate 0.04%, magnesium sulfate 0.03%, sodium chloride 0.03%, ferrous sulfate 0.001 %, calcium chloride 0.02%, add water to 1000mL, pH7.3~7.4; sterilize at 1...

Embodiment 2

[0055] The composition of the seed medium is by weight (w / w): The composition of the seed medium is by weight (w / w): glucose 2%, ammonium sulfate 0.7%, yeast extract powder 0.4%, magnesium sulfate 0.1%, Potassium dihydrogen phosphate 0.03%, add water to 1000mL, pH7.3~7.4; sterilize at 121℃, 0.15Mpa for 20min;

[0056] The amount of inoculum is to inoculate one test tube slant strain after activation (transfer the test tube slant strain stored on nutrient agar medium at 4°C to room temperature for activation for 4h-8h) per Erlenmeyer flask (100mL culture medium). The culture temperature is 35°C, the shaker speed is 160r / min, the culture time is 14h, and the seed solution OD 600 is 0.8;

[0057] Submerged fermentation medium composition, by weight (w / w): corn flour 1%, soybean meal 1.4%, ammonium nitrate 0.5%, manganese sulfate 0.01%, magnesium sulfate 0.08%, sodium chloride 0.01%, ferrous sulfate 0.004 %, calcium chloride 0.05%, add water to 1000mL, pH7.3~7.4; sterilize at 12...

Embodiment 3

[0064] The composition of the seed medium, by weight (w / w): glucose 1%, ammonium sulfate 0.8%, yeast extract powder 0.2%, magnesium sulfate 0.09%, potassium dihydrogen phosphate 0.01%, add water to 1000mL, pH7.3~ 7.4; 121℃, 0.15Mpa sterilization for 20min;

[0065] The amount of inoculum is to inoculate one test tube slant strain after activation (transfer the test tube slant strain stored on nutrient agar medium at 4°C to room temperature for activation for 4h-8h) per Erlenmeyer flask (100mL culture medium). The culture temperature is 30°C, the shaker speed is 180r / min, the culture time is 18h, and the seed solution OD 600 is 0.5;

[0066] Submerged fermentation medium composition, by weight (w / w): corn flour 2%, soybean meal 1.6%, ammonium nitrate 0.2%, manganese sulfate 0.05%, magnesium sulfate 0.05%, sodium chloride 0.05%, ferrous sulfate 0.003 %, calcium chloride 0.01%, add water to 1000mL, pH7.3~7.4; sterilize at 121℃, 0.15Mpa for 20min;

[0067] Submerged fermentatio...

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Abstract

The invention belongs to the field of environmental microorganisms and particularly relates to a thiourea degrading strain and a method for treating thiourea-containing wastewater by applying the same. The strain belongs to bacillus cereus and is preserved in the China Microbial Culture Collection Management Center, with a preservation number of CGMCC No.16152. The strain is applied to an aerobicbiochemical system of the thiourea-containing wastewater and can completely degrade thiourea and discharge ammonia nitrogen reaching standard; the situations that secondary pollution is caused by chemical treatment, and the wastewater discharge is unlikely to reach the standard are avoided. When being utilized for treating the thiourea-containing wastewater, the strain has the advantages of beinghigh in tolerance load, low in cost, good in treatment effect, stable in effluent quality and simple and convenient to operate and manage.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a thiourea-degrading bacterial strain and a method for using the bacterial strain to treat thiourea-containing wastewater. Background technique [0002] Thiourea, also known as thiourea, has the molecular formula CH 4 N 2 S, its application range is very wide, and its main uses are divided into three aspects: industry, medicine, and agriculture. It mainly exists as a nitrification inhibitor in agriculture, and the discharge in the production process is large. Due to the high toxicity of thiourea to microorganisms, when some thiourea enters the wastewater treatment plant, it is easy to inhibit the nitrification process, increase the ammonia nitrogen content in the effluent, make it difficult to meet the discharge requirements, and seriously affect the operation of the wastewater treatment plant. [0003] Zacherl et al. studied the inhibitory effect of thio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/30C12R1/085
CPCC02F3/34C02F2101/40C12N1/20C12N1/205C12R2001/085
Inventor 潘冬梅杨传伦栾波张心青倪建龙马娜娜王建平杨丹丹
Owner CHAMBROAD CHEM IND RES INST CO LTD
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