Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

magnetic particle buffer

A buffer solution and magnetic particle technology, applied in the field of immunodetection, can solve the problems of unfavorable detection results analysis, distortion of detection results, deviation of detection results, etc., to avoid abnormal detection results, improve detection sensitivity, and facilitate instrument sampling.

Active Publication Date: 2022-02-08
SICHUAN MACCURA BIOTECH CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the magnetic particles used in the magnetic particle chemiluminescence immunoassay are slightly soluble in water, easy to aggregate, and have poor dispersion, which causes the instrument to mistake multiple magnetic particles for one magnetic particle during detection, resulting in serious distortion of the detection results, which is very inaccurate. Facilitate the analysis of subsequent test results
Therefore, during the storage process, if the magnetic particles aggregate, they usually need to be re-dispersed before use. If there is no suitable tool or method, it is difficult to re-disperse uniformly. Although common methods such as high-speed stirring and ultrasonic dispersion can solve the dispersion problem of magnetic particles, However, high-speed stirring will generate bubbles, which will affect the test results. Ultrasonic dispersion will sometimes change the properties of magnetic particles, resulting in deviations in test results.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • magnetic particle buffer
  • magnetic particle buffer
  • magnetic particle buffer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] The research experiment of the pH value regulator in the embodiment 1 base buffer solution

[0076] Table 1 Study on the dosage of pH regulator

[0077]

Embodiment 2

[0078] The research experiment of osmotic pressure regulator in embodiment 2 base buffer solution

[0079] The consumption research of table 2 osmotic pressure regulator

[0080]

[0081]

Embodiment 3

[0082] The research experiment (1) of stabilizer in the embodiment 3 base buffer solution

[0083] The consumption research of table 3 mannitol

[0084]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a magnetic particle buffer, which contains a basic buffer, 0.005-0.08% (v / v) defoamer and 0.05-0.6 mol / L citrate. The magnetic particle buffer solution of the present invention has good dispersibility and is not easy to foam, and at the same time can significantly improve the detection sensitivity of the analyte in the sample.

Description

technical field [0001] The invention relates to the field of immunoassay, in particular to a magnetic particle buffer and application thereof. Background technique [0002] Growth hormone (Growth Hormone, GH) is a protein hormone secreted by the anterior pituitary gland with a growth-promoting function. Human Growth Hormone (hGH) consists of 191 amino acids and has a molecular weight of 22KD. It can promote substance metabolism It can affect the growth and development of various organs and tissues of the body, especially the bones, muscles and internal organs. [0003] At present, the laboratory detection methods of hGH include radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), time-resolved fluorescence analysis (TRFIA), chemiluminescence immunoassay (CLIA) and so on. RIA and ELISA appeared earlier, but due to the disadvantages of pollution, long time, and low sensitivity, they no longer occupy the dominant position. TRFIA has high requirements for detection equip...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/74G01N33/543G01N33/68
CPCG01N33/54326G01N33/68G01N33/74
Inventor 黄春田君喜张凌燕席强
Owner SICHUAN MACCURA BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com